| IED ID | IndEnz0002007557 |
| Enzyme Type ID | protease007557 |
| Protein Name |
RNA replicase polyprotein 206 kDa polyprotein Cleaved into: Methyltransferase/Protease EC 2.1.1.- EC 3.4.22.- 98 kDa protein MET/PRO ; Putative helicase EC 3.6.4.- 42 kDa protein HEL ; RNA-directed RNA polymerase EC 2.7.7.48 66 kDa protein POL |
| Gene Name | |
| Organism | Turnip yellow mosaic virus |
| Taxonomic Lineage | Viruses Riboviria Orthornavirae Kitrinoviricota Alsuviricetes Tymovirales Tymoviridae Tymovirus Turnip yellow mosaic virus |
| Enzyme Sequence | MAFQLALDALAPTTHRDPSLHPILESTVDSIRSSIQTYPWSIPKELLPLLNSYGIPTSGLGTSHHPHAAHKTIETFLLCTHWSFQATTPSSVMFMKPSKFNKLAQVNSNFRELKNYRLHPNDSTRYPFTSPDLPVFPTIFMHDALMYYHPSQIMDLFLRKPNLERLYASLVVPPEAHLSDQSFYPKLYTYTTTRHTLHYVPEGHEAGSYNQPSDAHSWLRINSIRLGNHHLSVTILESWGPVHSLLIQRGTPPPDPSLQAPPTLMTSDLFRSYQEPRLDVVSFRIPDAIELPQATFLQQPLRDRLVPRAVYNALFTYTRAVRTLRTSDPAAFVRMHSSKPDHDWVTSNAWDNLQTFALLNVPLRPNVVYHVLQSPIASLSLYLRQHWRRLTATAVPILSFLTLLQRFLPLPIPLAEVKSITAFRRELYRKKEPHHPLDVFHLQHRVRNYHSAISAVRPASPPHQKLPHALQKAALLLLRPISPLLTATPFFRSEQKSMLPNAELSWTLKRFALPWQASLVLLALSESSILLHKLFSPPTLQAQHDTYHRHLHPGSYSLQWERTPLSIPRTTAFLPFTPTTSTAPPDRSEASLPPAFASTFVPRPPPAASSPGAQPPTTTAAPPTPIEPTQRTHQNSDLALESSTSTEPPPPPIRSPDMTPSAPVLFPEINSPRRFPPQLPATPDLEPAHTPPPLSIPHQDPTDSADPLMGSHLLHHSLPAPPTHPLPSSQLLPAPLTNDPTAIGPVLPFEELHPRRYPENTATFLTRLRSLPSNHLPQPTLNCLLSAVSDQTKVSEEHLWESLQTILPDSQLSNEETNTLGLSTEHLTALAHLYNFQATVYSDRGPILFGPSDTIKRIDITHTTGPPSHFSPGKRLLGSQPSAKGHPSDPLIRAMKSFKVSGNYLPFSEAHNHPTSISHAKNLISNMKNGFDGVLSLLDVSTGQRTGPTPKERIIQIDHYLDTNPGKTTPVVHFAGFAGCGKTYPIQQLLKTKLFKDFRVSCPTTELRTEWKTAMELHGSQSWRFNTWESSILKSSRILVIDEIYKMPRGYLDLSILADPALELVIILGDPLQGEYHSQSKDSSNHRLPSETLRLLPYIDMYCWWSYRIPQCIARLFQIHSFNAWQGVIGSVSTPHDQSPVLTNSHASSLTFNSLGYRSCTISSSQGLTFCDPAIIVLDNYTKWLSSANGLVALTRSRSGVQFMGPSSYVGGTNGSSAMFSDAFNNSLIIMDRYFPSLFPQLKLITSPLTTRGPKLNGATPSASPTHRSPNFHLPPHIPLSYDRDFVTVNPTLPDQGPETRLDTHFLPPSRLPLHFDLPPAITPPPVSTSVDPPQAKASPVYPGEFFDSLAAFFLPAHDPSTREILHKDQSSNQFPWFDRPFSLSCQPSSLISAKHAPNHDPTLLPASINKRLRFRPSDSPHQITADDVVLGLQLFHSLCRAYSRQPNSTVPFNPELFAECISLNEYAQLSSKTQSTIVANASRSDPDWRHTTVKIFAKAQHKVNDGSIFGSWKACQTLALMHDYVILVLGPVKKYQRIFDNADRPPNIYSHCGKTPNQLRDWCQEHLTHSTPKIANDYTAFDQSQHGESVVLEALKMKRLNIPSHLIQLHVHLKTNVSTQFGPLTCMRLTGEPGTYDDNTDYNLAVIYSQYDVGSCPIMVSGDDSLIDHPLPTRHDWPSVLKRLHLRFKLELTSHPLFCGYYVGPAGCIRNPLALFCKLMIAVDDDALDDRRLSYLTEFTTGHLLGESLWHLLPETHVQYQSACFDFFCRRCPRHEKMLLDDSTPALSLLERITSSPRWLTKNAMYLLPAKLRLAITSLSQTQSFPESIEVSHAESELLHYVQ |
| Enzyme Length | 1844 |
| Uniprot Accession Number | P10358 |
| Absorption | |
| Active Site | ACT_SITE 783; /note=For protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01074; ACT_SITE 869; /note=For protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01074 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539}; |
| DNA Binding | |
| EC Number | 2.1.1.-; 3.4.22.-; 3.6.4.-; 2.7.7.48 |
| Enzyme Function | FUNCTION: Acts as a cysteine protease, methyltransferase and deubiquitinase. The cysteine protease activity cleaves the polyprotein giving rise to mature proteins. The methyltransferase domain is probably involved in viral RNA capping. The deubiquitylating activity counteracts the degradation of the viral polymerase mediated by the host ubiquitin-proteasome system. The polymerase is thus stabilized and infectivity is increased. {ECO:0000269|PubMed:22117220}.; FUNCTION: RNA-directed RNA polymerase is responsible for the replication and transcription of the genome. {ECO:0000255|PROSITE-ProRule:PRU00539, ECO:0000269|PubMed:22117220}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 976..983; /note=ATP; /evidence=ECO:0000250 |
| Features | Active site (2); Beta strand (7); Chain (4); Compositional bias (4); Domain (5); Helix (7); Nucleotide binding (1); Region (2); Site (2) |
| Keywords | 3D-structure;ATP-binding;Host-virus interaction;Hydrolase;Methyltransferase;Modulation of host ubiquitin pathway by viral deubiquitinase;Modulation of host ubiquitin pathway by virus;Nucleotide-binding;Nucleotidyltransferase;Protease;RNA-directed RNA polymerase;Reference proteome;Thiol protease;Transferase;Viral RNA replication |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: [Methyltransferase/Protease]: Host chloroplast envelope.; SUBCELLULAR LOCATION: [Putative helicase]: Host chloroplast envelope.; SUBCELLULAR LOCATION: [RNA-directed RNA polymerase]: Host chloroplast envelope. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (4) |
| Cross Reference PDB | 4A5U; 5LW5; 5LWA; 6YPT; |
| Mapped Pubmed ID | 22505427; 23966860; 25849500; 29117247; |
| Motif | |
| Gene Encoded By | |
| Mass | 206,641 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:21248 |
| Cross Reference Brenda |