| IED ID | IndEnz0002007583 |
| Enzyme Type ID | protease007583 |
| Protein Name |
HTH-type quorum-sensing regulator RhlR Elastase modulator Regulatory protein RhlR |
| Gene Name | rhlR lasM vsmR PA3477 |
| Organism | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Pseudomonadales Pseudomonadaceae Pseudomonas Pseudomonas aeruginosa group Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) |
| Enzyme Sequence | MRNDGGFLLWWDGLRSEMQPIHDSQGVFAVLEKEVRRLGFDYYAYGVRHTIPFTRPKTEVHGTYPKAWLERYQMQNYGAVDPAILNGLRSSEMVVWSDSLFDQSRMLWNEARDWGLCVGATLPIRAPNNLLSVLSVARDQQNISSFEREEIRLRLRCMIELLTQKLTDLEHPMLMSNPVCLSHREREILQWTADGKSSGEIAIILSISESTVNFHHKNIQKKFDAPNKTLAAAYAAALGLI |
| Enzyme Length | 241 |
| Uniprot Accession Number | P54292 |
| Absorption | |
| Active Site | |
| Activity Regulation | ACTIVITY REGULATION: Activated by interaction with the autoinducer signal molecule N-butanoyl-L-homoserine lactone (C4-HSL or BHL), the product of the RhlI synthase (PubMed:7604006, PubMed:14526008, PubMed:26732761). Is also activated by binding to rosmarinic acid (RA), a homoserine lactone mimic produced by plants, which induces a broad quorum sensing response, including the induction of all major quorum sensing controlled virulence factors. Rosmarinic acid secretion may be a plant defense mechanism to stimulate a premature quorum sensing response (PubMed:26732761, PubMed:30051572). {ECO:0000269|PubMed:14526008, ECO:0000269|PubMed:26732761, ECO:0000269|PubMed:30051572, ECO:0000269|PubMed:7604006}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | DNA_BIND 198..217; /note=H-T-H motif; /evidence=ECO:0000255|PROSITE-ProRule:PRU00411 |
| EC Number | |
| Enzyme Function | FUNCTION: Quorum-sensing regulator that controls the expression of multiple virulence factors in response to extracellular signaling molecules called autoinducers (PubMed:7494482, PubMed:8522523, PubMed:15576196). Involved, among others, in the transcriptional regulation of genes that are responsible for rhamnolipid surfactant biosynthesis (PubMed:8144472, PubMed:7604006, PubMed:14526008). Acts by binding to a specific sequence in the rhlAB regulatory region, both in the presence and in the absence of its autoinducer (PubMed:14526008). In the former case it activates transcription of the promoter, whereas in the latter it acts as a transcriptional repressor (PubMed:14526008). Also regulates the expression of the rmlBDAC operon, encoding dTDP-L-rhamnose biosynthetic enzymes, by binding to the rml box in the promoter region (PubMed:22262098). In addition, is involved in the regulation of the production of elastase (lasB) and pyocyanine (PubMed:8144472, PubMed:7604006, PubMed:8522523). {ECO:0000269|PubMed:14526008, ECO:0000269|PubMed:15576196, ECO:0000269|PubMed:22262098, ECO:0000269|PubMed:7494482, ECO:0000269|PubMed:7604006, ECO:0000269|PubMed:8144472, ECO:0000269|PubMed:8522523}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); DNA binding (1); Domain (1); Sequence conflict (3) |
| Keywords | Activator;Cytoplasm;DNA-binding;Quorum sensing;Reference proteome;Repressor;Transcription;Transcription regulation |
| Interact With | |
| Induction | INDUCTION: The rhlR promoter region contains four different transcription start sites, two of which are included in the upstream gene (rhlB) coding region. The rhlR gene is subject to a complex transcriptional regulation. Expression is dependent on LasR and on different regulatory proteins such as Vfr and RhlR itself, and also on the alternative sigma factor sigma(54). Expression is partially LasR-independent under certain culture conditions and is strongly influenced by environmental factors. {ECO:0000269|PubMed:14600219}. |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:12657054}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 27,578 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |