| IED ID | IndEnz0002007612 |
| Enzyme Type ID | protease007612 |
| Protein Name |
RHOMBOID-like protein 2 AtRBL2 EC 3.4.21.105 |
| Gene Name | RBL2 At1g63120 F16M19.4 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MANRDVERVGKKNRGANNNYFYEESSGETHWTSWLIPAIVVANLAVFIAVMFVNDCPKKITGPNKECVARFLGRFSFQPLKENPLFGPSSSTLEKMGALEWRKVVHEHQGWRLLSCMWLHAGIIHLLTNMLSLIFIGIRLEQQFGFIRVGLIYLISGLGGSILSSLFLQESISVGASGALFGLLGAMLSELLTNWTIYANKAAALITLLFIIAINLALGMLPRVDNFAHIGGFLTGFCLGFVLLVRPQYGWEASRTNTSRTKRKYSMYQYVLFVVSVVLLVVGLTVALVMLFKGENGNKHCKWCHYLSCFPTSKWTC |
| Enzyme Length | 317 |
| Uniprot Accession Number | Q9CAN1 |
| Absorption | |
| Active Site | ACT_SITE 177; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:P54493; ACT_SITE 229; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P54493 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.; EC=3.4.21.105; Evidence={ECO:0000269|PubMed:16223493}; |
| DNA Binding | |
| EC Number | 3.4.21.105 |
| Enzyme Function | FUNCTION: Rhomboid-type serine protease that catalyzes intramembrane proteolysis. Can cleave the Drosophila proteins Spitz and Keren (PubMed:16223493). May function in pollen elongation (PubMed:22007993). {ECO:0000269|PubMed:16223493, ECO:0000303|PubMed:22007993}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Transmembrane (7) |
| Keywords | Golgi apparatus;Hydrolase;Membrane;Protease;Reference proteome;Serine protease;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000269|PubMed:16223493}; Multi-pass membrane protein {ECO:0000255}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 17521445; 21798944; |
| Motif | |
| Gene Encoded By | |
| Mass | 35,413 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |