| IED ID | IndEnz0002007632 |
| Enzyme Type ID | protease007632 |
| Protein Name |
Rhomboid-like protease 5 EC 3.4.21.105 Microneme protein protease 1 MPP-1 |
| Gene Name | ROM5 MPP1 |
| Organism | Toxoplasma gondii |
| Taxonomic Lineage | cellular organisms Eukaryota Sar Alveolata Apicomplexa Conoidasida Coccidia Eucoccidiorida Eimeriorina Sarcocystidae Toxoplasma Toxoplasma gondii |
| Enzyme Sequence | MSSKGGSSRLGSKDLKKMTSRTERELRDSGRVRGEVERVEKRLRATAKVKEQPPTGDYKRRALASPGETAAPTFLVDSRGIPRKTSSTAPRKATLRPASSSPRLASSSRPTESTLPSSSSRALQGASSSSSSRPRRLHESASGRGGSGGSAGELRQEKKRLPELEAAEAAPASCVVELRDVTARKGRTSPATPPETAGSSVCGQGSHARTAEKLEEGTASHRDGSRRGSVDAETWATPGDGSSSHEFESSPQREERMQPQETGRRELSSEPRSGDLTKNGGDGGPRRHSCAWRKWREHMIQSFDITTHPFPPRGDGSPRRGKFLMIFLTSSVLFFVFLQELVLNVTTFNGRCMSPVLYPSHDAPESERTPRVISFGYGACEHNLGVSLFRREETKKDPRGRWTPGPLTERCASGRCASDDGWPSDLVQRGRAQRSPAAFDSPNPRVFSSLGALDTNKVRNYGEMFRVVWGMFLHGGWMHLLLNVSCQAQTLWILEPAWGFLRTLSLWIVGGVSGSLLSAVANPCTVTVGSSGAFYGLLGALVPFSIEYWDHIASPAWFLFCVSVLVMVAQFGNMVGVQGVDNNAHLGGLIGGLLFGFATIRSVHAFRWQGVAERMASSTLFWWMFPAEKRRSLREDNLQRVAREREERSSGRIPPPKFVWKFRGHEREWCVRFAAAVGLVTFWSVLWLYLLVPSYYESLSSPPGNFSFLGSTGCHCCRVQPFPGEEDKLPAFHPVRVNRGLFWCFVSEGVANLFCGRSSALNRGADVYGQTRQFEEALGDLPSARAGEAPLRIAKEEGESASVWQRLVKSAKKTYNAVLGNTTTPAAPSAAELAQQTRAGQ |
| Enzyme Length | 841 |
| Uniprot Accession Number | Q6GV23 |
| Absorption | |
| Active Site | ACT_SITE 531; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 585; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.; EC=3.4.21.105; |
| DNA Binding | |
| EC Number | 3.4.21.105 |
| Enzyme Function | FUNCTION: Serine protease involved in intramembrane proteolysis. Cleaves microneme adhesins, such as MIC2. This step is essential for efficient invasion of host cells. Catalyzes intramembrane proteolysis of AMA1. {ECO:0000269|PubMed:11927542, ECO:0000269|PubMed:15753289}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (5); Erroneous initiation (1); Mutagenesis (1); Region (1); Transmembrane (6) |
| Keywords | Hydrolase;Membrane;Protease;Serine protease;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Membrane {ECO:0000269|PubMed:15753289}; Multi-pass membrane protein {ECO:0000269|PubMed:15753289}. Note=Detected primarily at the posterior surface of intracellular tachyzoites. Detected in patches on the cell surface of extracellular tachyzoites. Concentrated at the posterior end of tachyzoites after induction of microneme secretion prior to invasion of host cells. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 92,102 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.4.21.105; |