Detail Information for IndEnz0002007651
IED ID IndEnz0002007651
Enzyme Type ID protease007651
Protein Name Metalloprotease AprA
EC 3.4.24.-
Gene Name aprA PSEEN1550
Organism Pseudomonas entomophila (strain L48)
Taxonomic Lineage cellular organisms Bacteria Proteobacteria Gammaproteobacteria Pseudomonadales Pseudomonadaceae Pseudomonas Pseudomonas entomophila Pseudomonas entomophila (strain L48)
Enzyme Sequence MSKVKESAIVSATSALQPQGPSSSYGLINSFAHQYDRGGANVNGKPSYTVDQAANYLLRDGAAWKDLNKDGTISLSYTFLTKAPSDFYSRGLGTFSQFSDLQKGQAKLAMQSWADVAKVTFTEAASGGDGHMTFGNFSASNGGAAFAYLPFDMPGSHKGESWYLINSSYQVNTTPGTGNYGRQTLTHEIGHVLGLSHPGDYNAGEGNPTYRDATYAQDTRGYSVMSYWSESNTGQNFVKAGGQYYASAPLMDDIAAIQKLYGANYATRSGDTVYGFNSNADRDFYSATSSSSKLVFSVWDGGGNDTFDFSGFTQNQKINLNETSFSDVGGMIGNVSIAKGVTIENAFGGSGNDLLIGNALANVLKGGAGNDIIYGGGGADQLWGGTGADTFVFGAISDSTKAAPDRIMDFTSGQDKIDLSAISAFAVNKLPLQFVNAFTGHAGEAVLSYDQGTNLGSLSIDFTGNSSADFLVTTVGQAAVTDIVV
Enzyme Length 485
Uniprot Accession Number Q1ID47
Absorption
Active Site ACT_SITE 188; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.4.24.-
Enzyme Function FUNCTION: Secreted protease which is important for P.entomophila to counteract the local immune response of Drosophila (PubMed:16789834). Can degrade antimicrobial peptides (AMPs), e.g. Diptericin and Cecropin A. Thus, protects P.entomophila from the Drosophila antimicrobial peptides produced by the gut innate immune response, and promotes bacterial persistence in the Drosophila gut and killing of the host (PubMed:16789834). Is responsible for maturation of pro-Monalysin to the active toxin Monalysin, by cleaving its N-terminus (PubMed:21980286). {ECO:0000269|PubMed:16789834, ECO:0000269|PubMed:21980286}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (1); Chain (1); Metal binding (45); Repeat (3)
Keywords Calcium;Hydrolase;Metal-binding;Metalloprotease;Protease;Repeat;Secreted;Toxin;Virulence;Zinc
Interact With
Induction INDUCTION: Is regulated by the GacS/GacA two-component system that controls P.entomophila pathogenicity. Seems also regulated by PrtR. {ECO:0000269|PubMed:16789834}.
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:16789834, ECO:0000269|PubMed:21980286}. Note=Is probably secreted via the associated Type 1 transporter encoded by aprDEF. {ECO:0000305|PubMed:16789834}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 50,931
Kinetics
Metal Binding METAL 187; /note=Zinc; via tele nitrogen; catalytic; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 191; /note=Zinc; via tele nitrogen; catalytic; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 197; /note=Zinc; via tele nitrogen; catalytic; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 268; /note=Calcium 1; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 270; /note=Calcium 1; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 272; /note=Calcium 1; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 300; /note=Calcium 1; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 302; /note=Calcium 1; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 303; /note=Calcium 2; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 305; /note=Calcium 1; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 305; /note=Calcium 2; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 342; /note=Calcium 2; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 344; /note=Calcium 2; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 349; /note=Calcium 3; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 351; /note=Calcium 3; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 353; /note=Calcium 3; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 358; /note=Calcium 4; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 360; /note=Calcium 4; via carbonyl oxygen; METAL 362; /note=Calcium 4; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 366; /note=Calcium 3; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 367; /note=Calcium 5; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 368; /note=Calcium 3; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 369; /note=Calcium 5; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 371; /note=Calcium 3; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 371; /note=Calcium 5; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 375; /note=Calcium 4; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 376; /note=Calcium 6; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 377; /note=Calcium 4; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 378; /note=Calcium 6; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 380; /note=Calcium 4; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 380; /note=Calcium 6; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 384; /note=Calcium 5; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 385; /note=Calcium 7; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 386; /note=Calcium 5; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 387; /note=Calcium 7; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 389; /note=Calcium 5; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 389; /note=Calcium 7; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 398; /note=Calcium 6; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 405; /note=Calcium 6; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 415; /note=Calcium 7; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 461; /note=Calcium 8; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 463; /note=Calcium 8; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 465; /note=Calcium 8; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 467; /note=Calcium 8; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q03023; METAL 469; /note=Calcium 8; /evidence=ECO:0000250|UniProtKB:Q03023
Rhea ID
Cross Reference Brenda