| IED ID | IndEnz0002007749 |
| Enzyme Type ID | protease007749 |
| Protein Name |
Arf-GAP with ANK repeat and PH domain-containing protein cnt-1 CED-3 protease suppressor Centaurin 1 Cleaved into: Truncated ctn-1 tCNT-1 |
| Gene Name | cnt-1 cps-2 Y17G7B.15 |
| Organism | Caenorhabditis elegans |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Nematoda (roundworms) Chromadorea Rhabditida Rhabditina Rhabditomorpha Rhabditoidea Rhabditidae Peloderinae Caenorhabditis Caenorhabditis elegans |
| Enzyme Sequence | MTVSNNGMAMPSVPPKIDCSEAIQDSPKFRATVAQHAMYFNRLENRLNEMLRHITAMIDFSKNYTNTFYKLTVSVNQLCDESFSGNPLASTTFQGLSEAYAHTVNLFRTYFDHSNVVTFTKLSNFIKIELTKVAESRAHFENMSQSMDDALVKNASISRQKPADATEGRNALTAVGTCFAHTTLDYVANINIAHAHKDHMILDALWTLVRESSAFFSKGHATFDEWTAADNGAIADTIQTFAAKSKLIERKMQDVHSLVPKEMFQHPSGMPIEPDVMMEGYLYKRSSNAFKTWNRRWFQIKDKQLLYSHRSTDLEPATIMEENLRICLVRPAPSNIDRIGCFELVTPTRIHLLQADSESLCQDWMRALQRTILALHEGDSVDVASTSPRNKTTSMSSGVTLTSANAISPLSNAMDVTKGRSVSDPASTYTSANTSSISTAAGFSSSTTAFEQVRRVPGNEVCADCGSPAPKWVSINLGVVLCIECSGAHRSLGVQTSKVRSLCMDSIDNELRDVLLALGNRQVNEIFLAHLPPADSIVPPQINEKSARPAREAWIKAKYVERRFAVAEDTRARSSATNRQEHLKHKTSIGGNSSSNGVNRSSSYADVQDAESGGLLDADPWSADLSVPVPTASKRLSACGSDTNLDAIGSSSIDTKTVEWDSVKEACECGDLLALMTAYAQGFDLNALHNGTTALHIATRNGQTAAVEFLLLNGAKINMLDEKLNTPLHLAAKEGHTLPVCQLLKRGADSNLANVDSKTPLDIAMECTHADIVTLFRVTIMRNDFNADFNNPMDETVEAVISDIARRAATEKEQKKTESLKSTSDI |
| Enzyme Length | 826 |
| Uniprot Accession Number | Q9XXH8 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: GTPase-activating protein for the ADP ribosylation factor family (Probable). Regulates endosome recycling downstream of rab-10 and upstream of arf-6 (PubMed:22869721). {ECO:0000269|PubMed:22869721, ECO:0000305|PubMed:22869721}.; FUNCTION: Truncated cnt-1: Promotes apoptosis during embryonic development. Produced by caspase ced-3-mediated cleavage, and translocates to the plasma membrane where it prevents the activation of the prosurvival Akt-1/2 and sgk-1 signaling pathway by competing with Akt-1/2 for the binding to PtdIns(3,4,5)P3. {ECO:0000269|PubMed:25383666}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Alternative sequence (1); Chain (2); Compositional bias (2); Domain (2); Mutagenesis (4); Region (1); Repeat (3); Sequence conflict (4); Site (2); Zinc finger (1) |
| Keywords | ANK repeat;Alternative splicing;Apoptosis;Cell membrane;Cytoplasm;Endocytosis;Endosome;Lipid-binding;Membrane;Metal-binding;Protein transport;Reference proteome;Repeat;Transport;Zinc;Zinc-finger |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:25383666}. Recycling endosome membrane {ECO:0000269|PubMed:22869721}; Peripheral membrane protein {ECO:0000269|PubMed:22869721}. Basolateral cell membrane {ECO:0000269|PubMed:22869721}; Peripheral membrane protein {ECO:0000269|PubMed:22869721}. Apical cell membrane {ECO:0000269|PubMed:22869721}; Peripheral membrane protein {ECO:0000269|PubMed:22869721}. Note=Colocalizes with rab-10, rab-8, rab-35 and clathrin chc-1 on recycling endosomes in the intestinal epithelium. Partially colocalizes with early endosome marker rab-5 and to a lesser extent with late endosome marker rme-1. Colocalizes with arf-6 on endosomes of the basolateral recycling pathway. {ECO:0000269|PubMed:22869721}.; SUBCELLULAR LOCATION: [Truncated ctn-1]: Cell membrane {ECO:0000269|PubMed:25383666}; Peripheral membrane protein {ECO:0000269|PubMed:25383666}. Note=Binds PtdIns(3,4,5)P3 at the plasma membrane. {ECO:0000269|PubMed:25383666}. |
| Modified Residue | |
| Post Translational Modification | PTM: Cleaved by caspase ced-3 after Asp-382 and Asp-609. Cleavage at Asp-382 is required for subsequent cleavage at Asp-609. {ECO:0000269|PubMed:25383666}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10778742; 11381264; 12529643; 14704431; 15122042; 15489339; 17164286; 19123269; 19343510; 20439776; 21085631; 21177967; 21367940; 22031882; 22267497; 22286215; 22347378; 22560298; 23144747; 23800452; 24884423; 25487147; 25491313; 25853217; 30220571; 6593563; |
| Motif | |
| Gene Encoded By | |
| Mass | 90,583 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |