| IED ID |
IndEnz0002007846 |
| Enzyme Type ID |
protease007846 |
| Protein Name |
pH-response transcription factor pacC/RIM101
|
| Gene Name |
RIM101 RPH2 YALI0B13640g |
| Organism |
Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica) |
| Taxonomic Lineage |
cellular organisms
Eukaryota
Opisthokonta
Fungi
Dikarya
Ascomycota
saccharomyceta
Saccharomycotina (true yeasts)
Saccharomycetes
Saccharomycetales
Dipodascaceae
Yarrowia
Yarrowia lipolytica (Candida lipolytica)
Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
|
| Enzyme Sequence |
MASYPYLAQSQPPQQQQQQQQQPQQQSQQLPTTAPSAAPQVNNTTANKPLYPASPNSPISPSDYSANMNVGGDSVDMLLSSVSAHHRSSDAGQSDMGSISPSTAHTTPDATTYKTSDEEDATGKITTPRSEGSPNTNGSGSDGENLVCKWGPCGKTFGSAEKLYAHLCDAHVGRKCTHNLSLVCNWDNCGIVTVKRDHITSHIRVHVPLKPYKCDFCTKSFKRPQDLKKHVKTHADDNEQAHNAYAKPHMQHTHQQQQQQQRYMQYPTYASGYEYPYYRYSQPQVQVPMVPSYAAVGHMPTPPMHPHAPIDRKRQWDTTSDFFDDIKRARVTPNYSSDIASRLSTIEQYIGIQGQQQQASPTPQTATTTSATPAPAAPHQATPPQQQLPSFKQGDYQETDQFLNQLGSNIYGNIKSVDPQYEAPAEFHLPHPMGYRYAFSHAPAPHGAAPVAPQVAPPAHPGVHGVSAPHYPDLSYSRSTVPQLSSRFEDVRQMSVGVTQRAARTTNVEESDDDDELVEGFGKMAIADSKAMQVAQMKKHLEVVSYLRRVLQEARETESGEAEDTAANKDTSASKSSLYPTIKAC |
| Enzyme Length |
585 |
| Uniprot Accession Number |
P78978 |
| Absorption |
|
| Active Site |
|
| Activity Regulation |
|
| Binding Site |
|
| Calcium Binding |
|
| catalytic Activity |
|
| DNA Binding |
|
| EC Number |
|
| Enzyme Function |
FUNCTION: Transcription factor that mediates regulation of both acid- and alkaline-expressed genes in response to ambient pH. At alkaline ambient pH, activates transcription of alkaline-expressed genes (including RIM101 itself) and represses transcription of acid-expressed genes, thereby regulating the synthesis and secretion of alkaline protease XPR2 and acidic protease AXP1 depending on ambient pH. Specifically recognizes and binds the consensus sequence 5'-GCCARG-3'. Required for mating and sporulation. {ECO:0000269|PubMed:11861549, ECO:0000269|PubMed:9199331}. |
| Temperature Dependency |
|
| PH Dependency |
|
| Pathway |
|
| nucleotide Binding |
|
| Features |
Chain (1); Compositional bias (3); Motif (2); Mutagenesis (3); Region (4); Zinc finger (3) |
| Keywords |
Activator;Cytoplasm;DNA-binding;Metal-binding;Nucleus;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;Zinc;Zinc-finger |
| Interact With |
|
| Induction |
|
| Subcellular Location |
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}. |
| Modified Residue |
|
| Post Translational Modification |
PTM: Activated by C-terminal proteolytic cleavage by signaling protease (probably palB/RIM13) at neutral to alkaline ambient pH. {ECO:0000250}. |
| Signal Peptide |
|
| Structure 3D |
|
| Cross Reference PDB |
- |
| Mapped Pubmed ID |
- |
| Motif |
MOTIF 471..474; /note=YPX[LI] motif 1; MOTIF 579..582; /note=YPX[LI] motif 2 |
| Gene Encoded By |
|
| Mass |
64,326 |
| Kinetics |
|
| Metal Binding |
|
| Rhea ID |
|
| Cross Reference Brenda |
|