| IED ID | IndEnz0002007863 |
| Enzyme Type ID | protease007863 |
| Protein Name |
Serine protease inhibitor Kazal-type 5 Lympho-epithelial Kazal-type-related inhibitor LEKTI Cleaved into: Hemofiltrate peptide HF6478; Hemofiltrate peptide HF7665 |
| Gene Name | SPINK5 |
| Organism | Homo sapiens (Human) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human) |
| Enzyme Sequence | MKIATVSVLLPLALCLIQDAASKNEDQEMCHEFQAFMKNGKLFCPQDKKFFQSLDGIMFINKCATCKMILEKEAKSQKRARHLARAPKATAPTELNCDDFKKGERDGDFICPDYYEAVCGTDGKTYDNRCALCAENAKTGSQIGVKSEGECKSSNPEQDVCSAFRPFVRDGRLGCTRENDPVLGPDGKTHGNKCAMCAELFLKEAENAKREGETRIRRNAEKDFCKEYEKQVRNGRLFCTRESDPVRGPDGRMHGNKCALCAEIFKQRFSEENSKTDQNLGKAEEKTKVKREIVKLCSQYQNQAKNGILFCTRENDPIRGPDGKMHGNLCSMCQAYFQAENEEKKKAEARARNKRESGKATSYAELCSEYRKLVRNGKLACTRENDPIQGPDGKVHGNTCSMCEVFFQAEEEEKKKKEGKSRNKRQSKSTASFEELCSEYRKSRKNGRLFCTRENDPIQGPDGKMHGNTCSMCEAFFQQEERARAKAKREAAKEICSEFRDQVRNGTLICTREHNPVRGPDGKMHGNKCAMCASVFKLEEEEKKNDKEEKGKVEAEKVKREAVQELCSEYRHYVRNGRLPCTRENDPIEGLDGKIHGNTCSMCEAFFQQEAKEKERAEPRAKVKREAEKETCDEFRRLLQNGKLFCTRENDPVRGPDGKTHGNKCAMCKAVFQKENEERKRKEEEDQRNAAGHGSSGGGGGNTQDECAEYREQMKNGRLSCTRESDPVRDADGKSYNNQCTMCKAKLEREAERKNEYSRSRSNGTGSESGKDTCDEFRSQMKNGKLICTRESDPVRGPDGKTHGNKCTMCKEKLEREAAEKKKKEDEDRSNTGERSNTGERSNDKEDLCREFRSMQRNGKLICTRENNPVRGPYGKMHINKCAMCQSIFDREANERKKKDEEKSSSKPSNNAKDECSEFRNYIRNNELICPRENDPVHGADGKFYTNKCYMCRAVFLTEALERAKLQEKPSHVRASQEEDSPDSFSSLDSEMCKDYRVLPRIGYLCPKDLKPVCGDDGQTYNNPCMLCHENLIRQTNTHIRSTGKCEESSTPGTTAASMPPSDE |
| Enzyme Length | 1064 |
| Uniprot Accession Number | Q9NQ38 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Serine protease inhibitor, probably important for the anti-inflammatory and/or antimicrobial protection of mucous epithelia. Contribute to the integrity and protective barrier function of the skin by regulating the activity of defense-activating and desquamation-involved proteases. Inhibits KLK5, it's major target, in a pH-dependent manner. Inhibits KLK7, KLK14 CASP14, and trypsin. {ECO:0000269|PubMed:10419450, ECO:0000269|PubMed:17596512, ECO:0000269|PubMed:20533828}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Alternative sequence (3); Beta strand (7); Chain (1); Compositional bias (1); Disulfide bond (32); Domain (15); Helix (12); Natural variant (13); Peptide (2); Region (6); Sequence conflict (1); Signal peptide (1); Site (1); Turn (5) |
| Keywords | 3D-structure;Alternative splicing;Cleavage on pair of basic residues;Direct protein sequencing;Disulfide bond;Hypotrichosis;Ichthyosis;Protease inhibitor;Reference proteome;Repeat;Secreted;Serine protease inhibitor;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | PTM: Proteolytically processed by furin in individual domains (D1, D5, D6, D8 through D11, and D9 through D15) exhibiting various inhibitory potentials for multiple proteases. {ECO:0000269|PubMed:17596512}. |
| Signal Peptide | SIGNAL 1..22; /evidence=ECO:0000255 |
| Structure 3D | NMR spectroscopy (6) |
| Cross Reference PDB | 1H0Z; 1HDL; 1UUC; 1UVF; 1UVG; 5YHN; |
| Mapped Pubmed ID | 11796258; 11841556; 11874482; 11943586; 12437098; 12752122; 12915442; 14551605; 15005725; 15304086; 15466487; 15675955; 15680911; 15820494; 15942217; 15986064; 16519819; 16601670; 17333166; 17415575; 17936012; 17989726; 17989887; 18617639; 18774391; 19118981; 19247692; 19438860; 19522716; 19534795; 19674346; 19913121; 19996348; 20085599; 20107740; 20395963; 20628086; 20701904; 20800603; 21087323; 21564178; 21585560; 22017185; 22089833; 22377713; 22570283; 22730493; 24015757; 24390132; 24577329; 24831437; 24894987; 25233048; 26031502; 26390218; 26865388; 27769847; 28213955; 28681574; 29926005; 30017565; 30270115; 30801672; 30868765; 31407378; 31450277; 31501002; 32564679; |
| Motif | |
| Gene Encoded By | |
| Mass | 120,714 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |