| IED ID | IndEnz0002007917 |
| Enzyme Type ID | protease007917 |
| Protein Name |
Serine protease inhibitor 2 Serpin 2 |
| Gene Name | SRPN2 1270169 AgaP_AGAP006911 |
| Organism | Anopheles gambiae (African malaria mosquito) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Nematocera Culicomorpha Culicoidea Culicidae (mosquitos) Anophelinae Anopheles Cellia Pyretophorus gambiae species complex Anopheles gambiae (African malaria mosquito) |
| Enzyme Sequence | MNKLNFVILCLAALLVFDATAQQDVHGPFQGQRQNEFDLMFVKEIFKNHNSNVVLSPFSVKILLTLIYEASDTSFGNAVSNTKRELSSVIQNDNIDHTRSYYKQLLESAQQDNKDYDLNIATNFFVDDFIEVINKYQQIANTHYHAMLEKVSYSNPTQTAATINNWVSEHTNGRLREIVTPDSLEGAVITLVNVIYFKGLWTYPFPEVANNVKPFYGTRGKPTNAQYMEQNGQFYYDNSADLGAQILRLPYRGNKLAMYFILPNPDNTVNQVLDRINSASLHQALWYMEENEVNVTLPKFKFDFSEQLNEPLQQVGIREIFSQNASLPLLARGRGARDEVRVSRIFQKAGITINELGSEAYAATEIQLVNKFGGDGVQIFNANRPFIFFIEDETLGTMLFAGKIENPVF |
| Enzyme Length | 409 |
| Uniprot Accession Number | Q7QIJ8 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Serine protease inhibitor that functions in the melanization-mediated immune response (PubMed:16113656, PubMed:20953892, PubMed:21465556, PubMed:25525260). By preventing the activation of phenoloxidases through the inhibiting of serine peptidase CLIPB9, negatively regulates melanization in the hemolymph (PubMed:16113656, PubMed:20953892, PubMed:21465556, PubMed:25525260). By preventing melanization, has a detrimental role during P.berghei parasite mediated-infection and invasion of the mosquito midgut (PubMed:16113656). {ECO:0000269|PubMed:16113656, ECO:0000269|PubMed:20953892, ECO:0000269|PubMed:21465556, ECO:0000269|PubMed:25525260}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Beta strand (17); Chain (1); Glycosylation (2); Helix (13); Motif (1); Mutagenesis (4); Sequence conflict (1); Signal peptide (1); Site (1); Turn (4) |
| Keywords | 3D-structure;Glycoprotein;Protease inhibitor;Reference proteome;Secreted;Serine protease inhibitor;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:16113656}. Note=Secreted into the hemolymph. {ECO:0000269|PubMed:16113656, ECO:0000269|PubMed:20953892}. |
| Modified Residue | |
| Post Translational Modification | PTM: PPeptidase CLIPB9 binds to SRPN2 via the hinge region resulting in the cleavage of the reactive bond (PubMed:25525260). This leads to a conformational change in SRPN2 which traps CLIPB9 and distorts its active site, resulting in CLIPB9 inactivation (PubMed:25525260). {ECO:0000269|PubMed:25525260}. |
| Signal Peptide | SIGNAL 1..21; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (5) |
| Cross Reference PDB | 3PZF; 4RO9; 4ROA; 4ROB; 4RSQ; |
| Mapped Pubmed ID | - |
| Motif | MOTIF 356..360; /note=Hinge region; required for binding to peptidase; /evidence=ECO:0000269|PubMed:25525260 |
| Gene Encoded By | |
| Mass | 46,512 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |