| IED ID | IndEnz0002007926 |
| Enzyme Type ID | protease007926 |
| Protein Name |
Microtubule-associated proteins 1A/1B light chain 3C Autophagy-related protein LC3 C Autophagy-related ubiquitin-like modifier LC3 C MAP1 light chain 3-like protein 3 MAP1A/MAP1B light chain 3 C MAP1A/MAP1B LC3 C Microtubule-associated protein 1 light chain 3 gamma |
| Gene Name | MAP1LC3C |
| Organism | Homo sapiens (Human) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human) |
| Enzyme Sequence | MPPPQKIPSVRPFKQRKSLAIRQEEVAGIRAKFPNKIPVVVERYPRETFLPPLDKTKFLVPQELTMTQFLSIIRSRMVLRATEAFYLLVNNKSLVSMSATMAEIYRDYKDEDGFVYMTYASQETFGCLESAAPRDGSSLEDRPCNPL |
| Enzyme Length | 147 |
| Uniprot Accession Number | Q9BXW4 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Ubiquitin-like modifier that plays a crucial role in antibacterial autophagy (xenophagy) through the selective binding of CALCOCO2 (PubMed:23022382). Recruits all ATG8 family members to infecting bacteria such as S.typhimurium (PubMed:23022382). May also play a role in aggrephagy, the macroautophagic degradation of ubiquitinated and aggregated proteins (PubMed:28404643). {ECO:0000269|PubMed:23022382, ECO:0000269|PubMed:28404643}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Beta strand (8); Chain (1); Helix (4); Lipidation (2); Modified residue (2); Mutagenesis (3); Propeptide (1); Site (1); Turn (1) |
| Keywords | 3D-structure;Autophagy;Cytoplasm;Cytoplasmic vesicle;Cytoskeleton;Lipoprotein;Membrane;Microtubule;Phosphoprotein;Reference proteome;Ubl conjugation pathway |
| Interact With | Q9BSB4; O75143; Q676U5; Q9Y4P1; Q13137; P20807-4; Q8WXU2; Q9BQS8; Q9UKD1; O00410; Q86V97; Q8WVZ9; O43561-2; Q8IVT2; Q14596; Q8TD19; O75323; P35372-10; Q9NS23; Q8WWW0; Q8NHV9; Q6ZNE9; Q15459; Q13501; Q13188; Q86VP1; Q3MII6; Q9UPU7; O15040; Q15025; Q96A56; Q969E8; Q9GZZ9; O75385; Q8IZQ1; P17028 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasmic vesicle, autophagosome membrane {ECO:0000269|PubMed:12740394, ECO:0000269|PubMed:20529957, ECO:0000269|PubMed:23459205, ECO:0000269|PubMed:31709703}; Lipid-anchor {ECO:0000269|PubMed:12740394}. Endomembrane system {ECO:0000269|PubMed:12740394}; Lipid-anchor {ECO:0000269|PubMed:12740394}. Cytoplasm, cytoskeleton {ECO:0000269|PubMed:12740394}. Note=LC3-II binds to the autophagic membranes. {ECO:0000269|PubMed:12740394}. |
| Modified Residue | MOD_RES 93; /note=Phosphoserine; by TBK1; /evidence=ECO:0000269|PubMed:31709703; MOD_RES 96; /note=Phosphoserine; by TBK1; /evidence=ECO:0000269|PubMed:31709703 |
| Post Translational Modification | PTM: The precursor molecule is cleaved by ATG4 (ATG4A, ATG4B, ATG4C or ATG4D) to expose the glycine at the C-terminus and form the cytosolic form, LC3-I (PubMed:15187094, PubMed:20818167, PubMed:30661429, PubMed:31709703). The processed form is then activated by APG7L/ATG7, transferred to ATG3 and conjugated to phosphatidylethanolamine (PE) phospholipid to form the membrane-bound form, LC3-II (PubMed:15187094). During non-canonical autophagy, the processed form is conjugated to phosphatidylserine (PS) phospholipid (PubMed:33909989). ATG4 proteins also mediate the delipidation of PE-conjugated forms (PubMed:33909989, PubMed:31709703). In addition, ATG4B and ATG4D mediate delipidation of ATG8 proteins conjugated to PS during non-canonical autophagy (PubMed:33909989). {ECO:0000269|PubMed:15187094, ECO:0000269|PubMed:20818167, ECO:0000269|PubMed:30661429, ECO:0000269|PubMed:31709703, ECO:0000269|PubMed:33909989}.; PTM: (Microbial infection) The Legionella effector RavZ is a deconjugating enzyme that hydrolyzes the amide bond between the C-terminal glycine residue and an adjacent aromatic residue in ATG8 proteins conjugated to phosphatidylethanolamine (PE), producing an ATG8 protein that is resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine (PubMed:23112293, PubMed:31722778). RavZ is also able to mediate delipidation of ATG8 proteins conjugated to phosphatidylserine (PS) (PubMed:33909989). {ECO:0000269|PubMed:23112293, ECO:0000269|PubMed:31722778, ECO:0000269|PubMed:33909989}.; PTM: Phosphorylation at Ser-96 and Ser-98 by TBK1 prevents interaction with ATG4 (ATG4A, ATG4B, ATG4C or ATG4D) (PubMed:31709703). Phosphorylation by TBK1 on autophagosomes prevents their delipidation by ATG4 and premature removal from nascent autophagosomes (PubMed:31709703). {ECO:0000269|PubMed:31709703}. |
| Signal Peptide | |
| Structure 3D | NMR spectroscopy (1); X-ray crystallography (4) |
| Cross Reference PDB | 2NCN; 3VVW; 3WAM; 3WAP; 5DPW; |
| Mapped Pubmed ID | 11825910; 18321988; 19250911; 19322194; 21177865; 22170151; 24300435; 24668264; 24747438; 25568151; 26431026; 27880899; 28655748; 29149599; 31578424; 31851933; 32065021; 32532970; 33081014; 33230801; 33932238; 33988680; |
| Motif | |
| Gene Encoded By | |
| Mass | 16,852 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |