| IED ID | IndEnz0002007945 |
| Enzyme Type ID | protease007945 |
| Protein Name |
Nuclear pore complex protein Nup98-Nup96 EC 3.4.21.- Cleaved into: Nuclear pore complex protein Nup98 98 kDa nucleoporin Nucleoporin Nup98 Nup98 ; Nuclear pore complex protein Nup96 96 kDa nucleoporin Nucleoporin Nup96 Nup96 |
| Gene Name | Nup98 |
| Organism | Mus musculus (Mouse) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse) |
| Enzyme Sequence | MFNKSFGTPFGGSTGGFGTTSTFGQNTGFGTTSGGAFGTSAFGSSNNTGGLFGNSQTKPGGLFGTSSFSQPATSTSTGFGFGTSTGTSNSLFGTASTGTSLFSSQNNAFAQNKPTGFGNFGTSTSSGGLFGTTNTTSNPFGSTSGSLFGPSSFTAAPTGTTIKFNPPTGTDTMVKAGVSTNISTKHQCITAMKEYESKSLEELRLEDYQANRKGPQNQVGGGTTAGLFGSSPATSSATGLFSSSTTNSAFSYGQNKTAFGTSTTGFGTNPGGLFGQQNQQTTSLFSKPFGQATTTPNTGFSFGNTSTLGQPSTNTMGLFGVTQASQPGGLFGTATNTSTGTAFGTGTGLFGQPNTGFGAVGSTLFGNNKLTTFGTSTTSAPSFGTTSGGLFGNKPTLTLGTNTNTSNFGFGTNNSGSSIFGSKPAAGTLGTGLGTGFGTALGAGQASLFGNNQPKIGGPLGTGAFGAPGFNTSTAILGFGAPQAPVALTDPNASAAQQAVLQQHLNSLTYSPFGDSPLFRNPMSDPKKKEERLKPTNPAAQKALTTPTHYKLTPRPATRVRPKALQTTGTAKSHLFDGLDDDEPSLANGAFMPKKSIKKLVLKNLNNSNLFSPVNHDSEDLASPSEYPENGERFSFLSKPVDENNQQDGEDDSLVSRFYTNPIAKPIPQTPESVGNKNNSSSNVEDTIVALNMRAALRNGLEGSSEETSFHDESLQDDREEIENNAYHIHPAGIVLTKVGYYTIPSMDDLAKITNEKGECIVSDFTIGRKGYGSIYFEGDVNLTNLNLDDIVHIRRKEVIVYVDDNQKPPVGEGLNRKAEVTLDGVWPTDKTSRCLIKSPDRLADINYEGRLEAVSRKQGAQFKEYRPETGSWVFKVSHFSKYGLQDSDEEEEEHPPKTTSKKLKTAPLPPAGQATTFQMTLNGKPAPPPQSQSPEVEQLGRVVELDSDMVDITQEPVPDSVLEESVPEDQEPVSASTHIASSLGINPHVLQIMKASLLVDEEDVDAMDQRFGHIPSKGETVQEICSPRLPISASHSSKSRSIVGGLLQSKFASGTFLSPSASVQECRTPRTSSRMNIPSTSPWSVPLPLATVFTVPSPAPEVQLKTVGIRRQPGLVPLEKSITYGKGKLLMDMALFMGRSFRVGWGPNWTLANSGEQLHGSHELENHQVADSMEYGFLPNPVAVKSLSESPFKVHLEKLGLRQRKLDEDLQLYQTPLELKLKHSTVHVDELCPLIVPNPGVSVIHDYADWVKDSPGDFLELPIVKHWSLTWTLCEALWGHLKELDGQLDEPSEYIQTLERRRAFSRWLSHTAAPQIEEEVSLTRRDSPVEAVFSYLTGSRISGACCLAQQSGDHRLALLLSQLVGSQSVRELLTMQLADWHQLQADSFIHDERLRIFALLAGKPVWQLSEQKQINVCSQLDWKRTLAIHLWYLLPPTASISRALSMYEEAFQNTPEGDKYACSPLPSYLEGCGCMVEEEKDSRRPLQDVCFHLLKLYSDRHYELNQLLEPRSITADPLDYRLSWHLWEVLRALNYTHLSEQCEGVLQASYAGQLESEGLWEWAIFVFLHIDNSGMREKAVRELLTRHCQLSETPESWAKEAFLTQKLCVPAEWIHEAKAVRAHMESNKHLEALYLFKAGHWNRCHKLVIRHLASDAIINENYDYLKGFLEDLAPPERSSLIQDWETSGLVYLDYIRVIEMLHRIQQVDCSGYELEHLHTKVTSLCNRIEQIPCYNAKDRLAQSDMAKRVANLLRVVLSLQHAPDATSNSTPDPQRVPLRLLAPHIGRLPMPEDYALEELRGLTQSYLRELTVGSQ |
| Enzyme Length | 1816 |
| Uniprot Accession Number | Q6PFD9 |
| Absorption | |
| Active Site | ACT_SITE 881; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:P52948 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. NUP98 and NUP96 are involved in the bidirectional transport across the NPC. May anchor NUP153 and TPR to the NPC. In cooperation with DHX9, plays a role in transcription and alternative splicing activation of a subset of genes. Involved in the localization of DHX9 in discrete intranuclear foci (GLFG-body). {ECO:0000250|UniProtKB:P52948}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (9); Chain (2); Compositional bias (2); Cross-link (3); Domain (1); Helix (4); Modified residue (22); Mutagenesis (1); Region (6); Sequence caution (3); Site (1); Turn (4) |
| Keywords | 3D-structure;Acetylation;Autocatalytic cleavage;Hydrolase;Isopeptide bond;Membrane;Nuclear pore complex;Nucleus;Phosphoprotein;Protease;Protein transport;Reference proteome;Repeat;Serine protease;Translocation;Transport;Ubl conjugation;mRNA transport |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Nucleus membrane {ECO:0000250|UniProtKB:P52948}; Peripheral membrane protein {ECO:0000250|UniProtKB:P52948}; Nucleoplasmic side {ECO:0000250|UniProtKB:P52948}. Nucleus, nuclear pore complex {ECO:0000250|UniProtKB:P52948}. Nucleus, nucleoplasm {ECO:0000250|UniProtKB:P52948}. Note=Localized to the nucleoplasmic side of the nuclear pore complex (NPC), at or near the nucleoplasmic basket. Dissociates from the dissasembled NPC structure early during prophase of mitosis. Colocalized with NUP153 and TPR to the nuclear basket of NPC. Colocalized with DHX9 in diffuse and discrete intranuclear foci (GLFG-body). Remains localized to the nuclear membrane after poliovirus (PV) infection. {ECO:0000250|UniProtKB:P52948}. |
| Modified Residue | MOD_RES 524; /note="Phosphoserine"; /evidence="ECO:0000250|UniProtKB:P52948"; MOD_RES 603; /note="N6-acetyllysine; alternate"; /evidence="ECO:0000250|UniProtKB:P52948"; MOD_RES 608; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:21183079"; MOD_RES 612; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:21183079"; MOD_RES 618; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:21183079"; MOD_RES 623; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:21183079"; MOD_RES 625; /note="Phosphoserine"; /evidence="ECO:0000250|UniProtKB:P52948"; MOD_RES 653; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:19144319, ECO:0007744|PubMed:21183079"; MOD_RES 670; /note="Phosphothreonine"; /evidence="ECO:0007744|PubMed:19144319"; MOD_RES 673; /note="Phosphoserine"; /evidence="ECO:0000250|UniProtKB:P52948"; MOD_RES 680; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:21183079"; MOD_RES 681; /note="Phosphoserine"; /evidence="ECO:0000250|UniProtKB:P52948"; MOD_RES 839; /note="Phosphoserine"; /evidence="ECO:0000250|UniProtKB:P52948"; MOD_RES 888; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:17242355, ECO:0007744|PubMed:19144319, ECO:0007744|PubMed:21183079"; MOD_RES 934; /note="Phosphoserine"; /evidence="ECO:0000250|UniProtKB:P52948"; MOD_RES 1027; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:17242355, ECO:0007744|PubMed:21183079"; MOD_RES 1042; /note="Phosphoserine"; /evidence="ECO:0000250|UniProtKB:P52948"; MOD_RES 1059; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:21183079"; MOD_RES 1063; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:21183079"; MOD_RES 1069; /note="Phosphothreonine"; /evidence="ECO:0000250|UniProtKB:P52948"; MOD_RES 1328; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:21183079"; MOD_RES 1771; /note="Phosphothreonine"; /evidence="ECO:0007744|PubMed:21183079" |
| Post Translational Modification | PTM: Autoproteolytically cleaved to yield Nup98 and Nup96 or Nup98 only, respectively. Cleaved Nup98 is necessary for the targeting of Nup98 to the nuclear pore and the interaction with Nup96. {ECO:0000250|UniProtKB:P52948}. |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 3TKN; |
| Mapped Pubmed ID | 10321731; 11248054; 12032333; 12477932; 12520002; 12543865; 12904583; 14610273; 15102471; 15226823; 15615787; 16033648; 16141072; 16355229; 16546098; 17442773; 17545593; 17967808; 18539113; 18799693; 19000832; 19581287; 19841179; 20335358; 20360068; 20375145; 21130494; 21267068; 21311561; 21677750; 22323452; 22613470; 23131583; 24090997; 24449168; 24943837; 26202421; 26496610; 26510501; 26731471; 27889185; 28392395; 30683868; 30700838; 31427429; 32652111; 8995226; 9027506; |
| Motif | |
| Gene Encoded By | |
| Mass | 197,241 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |