| IED ID | IndEnz0002008002 |
| Enzyme Type ID | protease008002 |
| Protein Name |
Polyprotein pp220 220 kDa polyprotein Cleaved into: p34; p14; p37; p150; p5 |
| Gene Name | War-102 |
| Organism | African swine fever virus (isolate Warthog/Namibia/Wart80/1980) (ASFV) |
| Taxonomic Lineage | Viruses Varidnaviria Bamfordvirae Nucleocytoviricota Pokkesviricetes Asfuvirales Asfarviridae Asfivirus African swine fever virus (ASFV) African swine fever virus (isolate Warthog/Namibia/Wart80/1980) (ASFV) |
| Enzyme Sequence | MGNRGSSTSSRPPPSSEANIYAKLQDHIQRQTRPFSGGGYFNGGGDKNPVQHIKDYHIDSVSSKAKLRIIEGIIRAIAKIGFKVDTKQPIEDILKDIKKQLPDPRAGSTFVKNAEKQETVCKMIADAINQEFIDLGQDKLIDTTEGAASICRQIVLYINSLTHGLRAEYLDVHGSIENTLENIKLLNDAIKQLHERMVTEVTKAAPNEEVINAVTMIEAVYRRLLNEQNLQINILTNFIDNILTPTQKELDKLQTDEVDIIKLLNDTNSVLGTKNFGKVLSYTLCNLGIAASVANKINKALQKVGLKVEQYLQSKNWAEFDKELDLKRFSGLVSAENIAEFEKAVNLLRQTFNERHKILENSCAKKGGDEEKTPLDRRIEAQRLDRKHILMEFLNKSTQAYNDFLENVKKIGIKLVKEIALTPNITRLRDALSRINDMGTIALDLSLIGFYTNAAAREERETFLTQFMLVKNVLEEQSKIDPNFKNLYDSCSRLLQIIDFYTDIVQKKYGGGEDCECTRVGGAALTVEELGLSKAARSQVDLNQAINTFMYYYYVAQIYSNLTHNKQEFQSYEENYATILGDAIAGRLMQLDTEKNARINSPAVDLARGHVGPNPGGAQEEDWKATVSAIELEYDVKRRFYRALEGLDLYLKNITKTFVNNIDSIQTVQQMLDGVRIIGRWFTEATGDTLAQVFESFPTSAGNDSNVFTDNAPAGHYYEKVAAEIQQGRSVGTLRPVRASQAKNIRDLIGRSLSNFQALKNIINAFARIGDMLGGEELRQMVPMSPLQIYKTLLEYIQHSALSVGLKNLNQSEIGGQRVALARTPEEAAQRVYLSTVRVNDALSTRWETEDVFFTFMLKSMAAKIFIVLGIYDMFERPEPVYKLIPTRMILGGADELEPEVIPEAAELYFRLPRLAEFYQKLFSFRDENVQISMLPELEGIFSGLIRIIFMRPIELINIGDYSETEIRQLIKEINVIYQHFNLEYGEQEATKKALIHFVNEINRRFGVITRTEWEKFQRIVQEARTMNDFGMMNQTNYSILPDEDGYTQSSQLLPSDRFISPSSQPTPKWRPALYNIDSVDVQTGMLQPNSQWDLVQKFRKQLSEMFEDPSLQQELGKVSYQELIRQAINELKKDHTDKIQIVSKLIQGSESLADTDVNKIFLFHETVITGLNLLSAIYVLLNNFRNNIKGLDLDTIQKSIIEWLRETQAANVNHANLIDWLGRKHGAISEIRNPGLVVKENDARLSRVYPDPTTNATAPQDQNLVTETLFAWIVPYVGIPAGGGVRAEQELAARYLVDNQRIMQLLLTNIFEMTSSFNKMVQVRFPETSTAQVHLDFTGLISLIDSLMADTKYFLNLLRPHIDKNIIQYYENRSNPGSFYWLEEHLIDKLIKPPTDAGGRPLPGGELGLEGVNQIINKTYTLLTKPYNVLQLQGGAQRRDAANIQINNNPQPSERFEQYGRVFSRLVFYDALENNSGLRVEQVVLGDFRLSNLIRTNNAQEENALSYWDNIALRTYANVNDAANNLRRYRLYGSDHGIQNNRSMMMVFNQLVASYIARFYDAPSGKIYLNLINAFANGNFSQAVMEMGYAHPDLARNNNAFGHRGDPTEQSVLLLSLGLILQRLIKDTNRQGLSQHLISTLTEIPIYLKENYRANLPLFNKMFNILISQGELLKQFIQYTNVQLARPNLMGLLGANNDSIIYYNNNNVPTTGLSVGQAALRGIGGVFRPNVTLMPLGDAQNNTSDIVRKRLVAVIDGIIRGSHTLADSAMEVLHELTDHPIYLETEEHFIQNYMSRYNKEPLMPFSLSLYYLRDLRIENNEVYDPLLYPNLESGSPEFKLLYGTRKLLGNDPVQLSDMPGVQLIMKNYNETVVAREQITPTRFEHFYTHAIQALRFIINIRSFKTVMMYNENTFGGVNLISENRDDKPIITAGIGMNAVYSLRKTLQDVISFVESSYQEEQINHIHKIVSPKGQTRTLGSNRERERIFNLFDMNIIPINVNALMRSIPLANIYNYDYSFEEIACLMYGISAEKVRSLDTTAPQPDVAEVLNIPNRPPINTREFMLKLLINPYVSVSITQYGNELLSKGNAGYMSRIFRGDNALNMGRPKFLSDQIFNKVLFGSLYPTQFDYDEAGPGLAAGIQRGRERWGHPMSIYINQALHEIVRTIRLAETVRGLRNVIDRNQIIGELNAFRTQLEDTRREVNNLIQTPEIQNNPTPEIIAAIQNWVQQYRGQITNLIDLIGNAGQANSMINLIQNITPQTAGAQLTALFNIRGLPAPPPRQPLQNDIEAMQWFMTIVINHPPILIAPFMLLVNNLKEFLNTLERYVYKTPRWLGPGTARIAQPPVGMAPGINMRHHTSYTENSVLTYITEQNREEGPWSIVKQVGVGIQKPTLVHIGKDRFDTRLIRNLIFITNIQRLLRLRLNLELSQFRNVLVSPDHIINPSITEYGFSITGPSETFSDKQYDSDIRIL |
| Enzyme Length | 2475 |
| Uniprot Accession Number | P0CA04 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: [Polyprotein pp220]: Essential for the core assembly. Its myristoyl moiety may function as a membrane-anchoring signal to bind the developing core shell to the inner viral envelope. {ECO:0000250|UniProtKB:Q08358}.; FUNCTION: [p34]: The structural protein p34 is a component of the virus core shell. {ECO:0000250|UniProtKB:Q08358}.; FUNCTION: [p14]: The structural protein p14 is a component of the virus core shell. {ECO:0000250|UniProtKB:Q08358}.; FUNCTION: [p37]: The structural protein p37 is a component of the virus core shell. {ECO:0000250|UniProtKB:Q08358}.; FUNCTION: [p150]: The structural protein p150 is a component of the virus core shell. {ECO:0000250|UniProtKB:Q08358}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (6); Coiled coil (1); Initiator methionine (1); Lipidation (1); Site (4) |
| Keywords | Coiled coil;Host cytoplasm;Host nucleus;Late protein;Lipoprotein;Myristate;Reference proteome;Virion |
| Interact With | |
| Induction | INDUCTION: [Polyprotein pp220]: Expressed in the late phase of the viral replicative cycle. {ECO:0000250|UniProtKB:Q08358}. |
| Subcellular Location | SUBCELLULAR LOCATION: [Polyprotein pp220]: Host cytoplasm, host perinuclear region {ECO:0000250|UniProtKB:Q08358}. Note=Found in perinuclear cytoplasmic viral factories during assembly. {ECO:0000250|UniProtKB:Q08358}.; SUBCELLULAR LOCATION: [p34]: Virion {ECO:0000250|UniProtKB:Q08358}. Host cytoplasm, host perinuclear region {ECO:0000250|UniProtKB:Q08358}. Note=Localizes to the viral factory at 16 hpi (By similarity). In the virion, located in the core shell, which functions like a matrix between the DNA-containing nucleoid and the inner envelope (By similarity). {ECO:0000250|UniProtKB:Q08358}.; SUBCELLULAR LOCATION: [p14]: Virion {ECO:0000250|UniProtKB:Q08358}. Host cytoplasm, host perinuclear region. Note=Found in perinuclear cytoplasmic viral factories during assembly. In the virion, located in the core shell, which functions like a matrix between the DNA-containing nucleoid and the inner envelope (By similarity). {ECO:0000250|UniProtKB:Q08358}.; SUBCELLULAR LOCATION: [p37]: Virion {ECO:0000250|UniProtKB:Q08358}. Host cytoplasm, host perinuclear region {ECO:0000250|UniProtKB:Q08358}. Host nucleus {ECO:0000250|UniProtKB:Q08358}. Note=Found in perinuclear cytoplasmic viral factories during assembly. In the virion, located in the core shell, which functions like a matrix between the DNA-containing nucleoid and the inner envelope (By similarity). {ECO:0000250|UniProtKB:Q08358}.; SUBCELLULAR LOCATION: [p150]: Virion {ECO:0000250|UniProtKB:Q08358}. Host cytoplasm, host perinuclear region {ECO:0000250|UniProtKB:Q08358}. Note=Found in perinuclear cytoplasmic viral factories during assembly. In the virion, located in the core shell, which functions like a matrix between the DNA-containing nucleoid and the inner envelope (By similarity). {ECO:0000250|UniProtKB:Q08358}.; SUBCELLULAR LOCATION: [p5]: Virion {ECO:0000250|UniProtKB:Q08358}. |
| Modified Residue | |
| Post Translational Modification | PTM: [Polyprotein pp220]: The polyprotein is not glycosylated.; PTM: [Polyprotein pp220]: Specific enzymatic cleavages in vivo by the viral pS273R protease yield mature proteins. {ECO:0000250|UniProtKB:Q08358}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 280,995 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |