IED Industry Enzyme Database

Detail Information for IndEnz0002008014
IED ID IndEnz0002008014
Enzyme Type ID protease008014
Protein Name Major prion protein
PrP
PrP27-30
PrP33-35C
CD antigen CD230
Gene Name Prnp Prn-p Prp
Organism Mus musculus (Mouse)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse)
Enzyme Sequence MANLGYWLLALFVTMWTDVGLCKKRPKPGGWNTGGSRYPGQGSPGGNRYPPQGGTWGQPHGGGWGQPHGGSWGQPHGGSWGQPHGGGWGQGGGTHNQWNKPSKPKTNLKHVAGAAAAGAVVGGLGGYMLGSAMSRPMIHFGNDWEDRYYRENMYRYPNQVYYRPVDQYSNQNNFVHDCVNITIKQHTVTTTTKGENFTETDVKMMERVVEQMCVTQYQKESQAYYDGRRSSSTVLFSSPPVILLISFLIFLIVG
Enzyme Length 254
Uniprot Accession Number P04925
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number
Enzyme Function FUNCTION: Its primary physiological function is unclear. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May promote myelin homeostasis through acting as an agonist for ADGRG6 receptor. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro) (By similarity). Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. Also provides Cu(2+) or Zn(2+) for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains (PubMed:12732622, PubMed:16492732, PubMed:19242475, PubMed:19568430). {ECO:0000250|UniProtKB:P04156, ECO:0000269|PubMed:12732622, ECO:0000269|PubMed:16492732, ECO:0000269|PubMed:19242475, ECO:0000269|PubMed:19568430}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Beta strand (4); Chain (1); Disulfide bond (1); Glycosylation (2); Helix (6); Lipidation (1); Metal binding (12); Modified residue (1); Mutagenesis (2); Natural variant (2); Propeptide (1); Region (4); Repeat (5); Sequence conflict (2); Signal peptide (1); Turn (1)
Keywords 3D-structure;Amyloid;Cell membrane;Copper;Direct protein sequencing;Disulfide bond;GPI-anchor;Glycoprotein;Golgi apparatus;Hydroxylation;Lipoprotein;Membrane;Metal-binding;Prion;Reference proteome;Repeat;Signal;Zinc
Interact With P12023; O08532; Q60631; Itself; P04156
Induction
Subcellular Location SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:11571277, ECO:0000269|PubMed:9837873}; Lipid-anchor, GPI-anchor. Golgi apparatus {ECO:0000269|PubMed:11756421}. Note=Targeted to lipid rafts via association with the heparan sulfate chains of GPC1. Colocates, in the presence of Cu(2+), to. vesicles in para- and perinuclear regions, where both proteins undergo internalization. Heparin displaces PRNP from lipid rafts and promotes endocytosis. {ECO:0000269|PubMed:11571277, ECO:0000269|PubMed:12732622, ECO:0000269|PubMed:16923158, ECO:0000269|PubMed:9837873}.
Modified Residue MOD_RES 44; /note=Hydroxyproline; /evidence=ECO:0000269|PubMed:11032800
Post Translational Modification PTM: N-glycosylated. {ECO:0000269|PubMed:16492732, ECO:0000269|PubMed:19349973, ECO:0000269|PubMed:19568430}.
Signal Peptide SIGNAL 1..22; /evidence=ECO:0000269|PubMed:2894984
Structure 3D NMR spectroscopy (15); X-ray crystallography (9)
Cross Reference PDB 1AG2; 1XYX; 1Y15; 1Y16; 2K5O; 2KFM; 2KFO; 2KU5; 2KU6; 2L1D; 2L1E; 2L1H; 2L1K; 2L39; 2L40; 3NVG; 3NVH; 4H88; 4J8R; 4MA7; 4MA8; 6AQ7; 6HER; 6HHD;
Mapped Pubmed ID 10037495; 10086355; 10102274; 10352232; 10378511; 10421360; 10461901; 10477271; 10521590; 10525406; 10529232; 10550328; 10581259; 10656806; 10708751; 10805813; 10842180; 10900000; 10930132; 10954563; 10985358; 10987828; 11038396; 11073804; 11130978; 11152514; 11152682; 11179214; 11208919; 11239719; 11259483; 11259607; 11263978; 11274428; 11278539; 11300723; 11300727; 11312611; 11333913; 11353827; 11359769; 11369504; 11546838; 11566872; 11697729; 11697909; 11716303; 11742063; 11743735; 11775003; 11782965; 11792852; 11823413; 11842265; 11846415; 11860274; 11867531; 11904434; 11912192; 11924867; 11950500; 12034448; 12051707; 12070160; 12084159; 12091459; 12093733; 12130636; 12163492; 12206674; 12215421; 12271119; 12379130; 12383347; 12386336; 12386337; 12387871; 12411613; 12425947; 12431994; 12435392; 12446843; 12456643; 12459456; 12481985; 12500977; 12519913; 12529324; 12556465; 12663673; 12664293; 12679564; 12684540; 12758067; 12759361; 12794132; 12805223; 12853456; 12871949; 12876477; 12878182; 12904479; 12911750; 12914944; 12927782; 12933795; 1353438; 1373228; 14511115; 14515989; 14525996; 14570892; 14573822; 14593181; 14597699; 14622086; 14625012; 14633630; 14645231; 14657385; 14668351; 14697265; 14706620; 14747574; 14752167; 14753456; 14999066; 15026410; 15050367; 15055597; 15082172; 15084583; 15090539; 15126640; 15133046; 15133048; 15146195; 15163699; 15170227; 15173173; 15176439; 15194501; 15208260; 15215178; 15229245; 15262264; 15262998; 15286374; 15301538; 15351724; 15354287; 15448157; 15451062; 15459186; 15467011; 15494440; 15509552; 15514463; 15528202; 15530652; 15539455; 15539564; 15542685; 15582152; 15596141; 15607951; 15615717; 15632154; 15647363; 15661974; 15670611; 15670743; 15671025; 15694261; 15706401; 15709746; 15753097; 15767405; 15800202; 15824101; 15827140; 15837581; 15846375; 15851519; 15862314; 15888663; 15890277; 15893468; 15911347; 15917101; 15917252; 15933194; 15936714; 15950943; 15951190; 15962001; 15970215; 16029203; 16084105; 16120605; 16135751; 16141072; 16157591; 16160162; 16177294; 16198494; 16219759; 16239348; 16253245; 16254249; 16258172; 16272297; 16278084; 16282497; 16294306; 16314415; 16339028; 16343423; 16386707; 16441239; 16467153; 16480953; 16517717; 16519879; 16537534; 16547418; 16554307; 16569635; 16574930; 16602821; 16621165; 16632309; 16635246; 1670551; 16707568; 1672162; 16730679; 16731968; 16738485; 16751368; 16766127; 1676894; 16787422; 1681406; 16825571; 16843609; 16844683; 1685143; 16866364; 1687379; 1687380; 1687381; 1687385; 16908519; 16912302; 16935263; 16940522; 16942751; 16945105; 16952355; 17056541; 17062642; 17084974; 17098996; 17106625; 17121821; 17131403; 17135402; 17144900; 17157195; 17165097; 17223707; 17241158; 17244617; 17245436; 17245437; 17251426; 17270731; 17292334; 17304577; 17320038; 17334982; 17337056; 17360589; 17379700; 17388948; 17405937; 17420473; 17433806; 17443816; 17446686; 17462540; 17468101; 17485546; 17494993; 17498663; 17504807; 17510630; 17531449; 17569776; 17570349; 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19283723; 19285121; 19286300; 19385058; 19393664; 19400950; 19457070; 19477226; 19479969; 19523487; 19524041; 19587281; 19597535; 19618915; 19655812; 19664595; 19666958; 19675240; 1968507; 19685161; 19700753; 1970329; 1970330; 19703931; 19709627; 19710140; 19720066; 19738901; 19747526; 19748890; 19760599; 19766283; 19766638; 19786843; 19805070; 19806224; 19823766; 19834742; 19843586; 19844576; 19846876; 19850936; 19854187; 19855845; 19888917; 19910576; 19940127; 20007469; 20040362; 20048341; 20075202; 20085368; 20086009; 20106973; 20110469; 20133875; 20138835; 20145049; 20161712; 20206606; 20219645; 20221436; 20238136; 20345906; 20356832; 20394048; 20395435; 20445063; 20460128; 20472884; 20541558; 20599662; 20610764; 20615969; 20626348; 20649983; 20660771; 20670217; 20679345; 20679477; 20703260; 20717837; 20808809; 20818492; 20876210; 20878768; 20930564; 20970434; 21041683; 21083616; 21123371; 21144827; 21156803; 21193014; 21195746; 21209925; 21212268; 21212283; 21214263; 21267068; 21277044; 21277354; 21323366; 21334441; 21335237; 21350487; 21357423; 21385869; 21406608; 21432853; 21439331; 21441896; 21441913; 21453198; 21466851; 21483752; 21555356; 21559407; 21586567; 21619885; 21641403; 21727894; 21743439; 21763679; 21766339; 21775587; 21777560; 21779169; 21795680; 21800884; 21835918; 21843375; 21847793; 21848803; 21858045; 21871438; 21900234; 21900252; 21909267; 21909425; 21912705; 21924230; 21931781; 21957246; 21957261; 21980292; 21980526; 21987789; 22025612; 22031932; 22038049; 22039779; 22043908; 22072688; 22072968; 22093825; 22100763; 22114562; 22144895; 22163178; 22184125; 22216002; 22222374; 22252492; 22285492; 22307640; 22331873; 22363722; 22398286; 22428057; 22447932; 22449963; 22493452; 22511770; 22529900; 22536327; 22537845; 22542184; 22586108; 22619325; 22648512; 22654104; 22679554; 22687393; 22705206; 22711839; 22743555; 22745483; 22762267; 22770220; 22772447; 22789566; 22820466; 22854022; 22860039; 22866057; 22889724; 22910907; 22912582; 22915801; 22922482; 22927985; 22930754; 22948149; 22956830; 22987112; 23002439; 23022479; 23061439; 23072804; 23121447; 23132868; 23133383; 23139828; 23145988; 23238082; 23256626; 23283973; 23290724; 23335053; 23345215; 23349890; 23386614; 23395905; 23407955; 23415788; 23481568; 23487467; 23580200; 23593266; 23629842; 23637596; 23650394; 23680490; 23729591; 23761404; 23764837; 23770331; 23787699; 23792955; 23805846; 23808898; 23811055; 23825952; 23831375; 23843953; 23851597; 23892077; 23903654; 23936187; 23940658; 23949728; 23959875; 23966386; 24000807; 24012003; 24012669; 24027305; 24038599; 24040215; 24086135; 24117858; 24133259; 24143866; 24145514; 24204258; 24225951; 24244171; 24247244; 24280226; 24307586; 24312586; 24329154; 24338008; 24352451; 24352465; 24373770; 24430187; 24431459; 24493590; 24497115; 24554723; 24574409; 24596090; 24604355; 24608126; 24631389; 24667414; 24685176; 24714645; 24752288; 24766164; 24810941; 24952961; 24965601; 25005024; 25034251; 25058617; 25139744; 25202268; 25242137; 25416551; 25426519; 25447819; 25490046; 25502554; 25510416; 25517431; 25539913; 25572394; 25584902; 25654651; 25658480; 25671600; 25673720; 25692227; 25695140; 25710374; 2575583; 25810062; 25810548; 25862412; 25972546; 26024859; 26152722; 26155834; 26159734; 26165121; 26175152; 26224882; 26250617; 26256540; 26287631; 26295309; 26298321; 26299705; 26423957; 26490404; 26500295; 26500296; 26553874; 26608316; 26627256; 26710111; 26713717; 26721882; 26857744; 26864450; 26873446; 26926995; 26949218; 27128441; 27208175; 27226369; 27227882; 27317661; 27325697; 27325698; 27327609; 27365298; 27440899; 27447728; 27463540; 27535221; 27550517; 27563063; 27591261; 27629560; 27629715; 27641601; 27689697; 27818203; 27849581; 27880767; 27891071; 27936017; 27973593; 27982059; 28178353; 28207746; 28527237; 28545141; 2859120; 28630311; 28669732; 28701513; 28736314; 28739602; 28760925; 28821618; 28827331; 2884041; 28842420; 28842494; 28851903; 28855681; 28874781; 28924012; 28945221; 29070671; 29220360; 29265373; 29337365; 29409004; 29569342; 29574582; 29577883; 29700113; 29700330; 29705776; 29748616; 29846863; 30054538; 30128651; 3015416; 30174115; 30275016; 30513342; 30729399; 30914754; 3094007; 30956132; 31041657; 31147460; 31161647; 31189938; 31291644; 3149717; 31511544; 31624071; 31666632; 31726043; 31815959; 31950499; 31985492; 31987501; 32012886; 32107004; 32165692; 32317725; 32394432; 32473880; 32561641; 32738196; 32829029; 32845931; 32861834; 32875355; 32895288; 32898162; 32959170; 32985542; 33137126; 33481951; 33547547; 33713980; 34502545; 34710372; 34810220; 34847154; 4970191; 6801762; 7494265; 7512619; 7553876; 7558020; 7622046; 7719349; 7842304; 7902565; 7904753; 7907357; 7911243; 7911437; 7912659; 7912827; 7913753; 7913762; 7962730; 7999308; 8030944; 8030958; 8095862; 8100741; 8118101; 8407244; 8462855; 8552188; 8602267; 8606772; 8617512; 8634020; 8635458; 8651915; 8698234; 8734907; 8757019; 8758005; 8790598; 8845956; 8917559; 8944151; 8982352; 9016962; 9030645; 9031631; 9163538; 9186056; 9207082; 9212101; 9242048; 9294164; 9294167; 9321385; 9333232; 9353594; 9363892; 9371744; 9414161; 9450236; 9462739; 9523587; 9568713; 9582258; 9590434; 9636277; 9751723; 9754876; 9770528; 9792790; 9867802; 9876879; 9883727; 9932423;
Motif
Gene Encoded By
Mass 27,977
Kinetics
Metal Binding METAL 60; /note=Cu(2+) 1; /evidence=ECO:0000250|UniProtKB:P04156; METAL 61; /note=Cu(2+) 1; via amide nitrogen; /evidence=ECO:0000250|UniProtKB:P04156; METAL 62; /note=Cu(2+) 1; via amide nitrogen and carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:P04156; METAL 68; /note=Cu(2+) 2; /evidence=ECO:0000250|UniProtKB:P04156; METAL 69; /note=Cu(2+) 2; via amide nitrogen; /evidence=ECO:0000250|UniProtKB:P04156; METAL 70; /note=Cu(2+) 2; via amide nitrogen and carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:P04156; METAL 76; /note=Cu(2+) 3; /evidence=ECO:0000250|UniProtKB:P04156; METAL 77; /note=Cu(2+) 3; via amide nitrogen; /evidence=ECO:0000250|UniProtKB:P04156; METAL 78; /note=Cu(2+) 3; via amide nitrogen and carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:P04156; METAL 84; /note=Cu(2+) 4; /evidence=ECO:0000250|UniProtKB:P04156; METAL 85; /note=Cu(2+) 4; via amide nitrogen; /evidence=ECO:0000250|UniProtKB:P04156; METAL 86; /note=Cu(2+) 4; via amide nitrogen and carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:P04156
Rhea ID
Cross Reference Brenda