| IED ID | IndEnz0002008061 |
| Enzyme Type ID | protease008061 |
| Protein Name |
RNA replicase polyprotein EC 2.7.7.48 |
| Gene Name | |
| Organism | Ononis yellow mosaic virus |
| Taxonomic Lineage | Viruses Riboviria Orthornavirae Kitrinoviricota Alsuviricetes Tymovirales Tymoviridae Tymovirus Ononis yellow mosaic virus |
| Enzyme Sequence | MAFELALNALASSTHKDSSLNPVLNSAVQPLQTSLQNFPWIIGKEHLPFLLAAGIPTSGFGCNPHPHAVHKVIETFLLFNHWSFMATVQASVMFMKPSKFKKLASVNPNFSELVNYRLTAADSVRYPSTSTSLPKYEIVFMHDALMYFNPSQILDLFIQCPSLQRLHCSLVVPPESSFTDLSLHPNLYTYTISGNTLHYVPEGHHAGSYDQPLDAISWLKLNSIHSPHLNLSVSKLESWGPVHSLLITRGLPHLPSSEKQQVSFHIPNCLPLPEATFLHQPLRHRLVPTEVYDALFTYTRAVRTLRTSDPAGFVRTHSNKPQYSWVTSRAWDNLQTYALLNAPVRPVVLFDFFLSPLKKFQLFMSQHINSLVIKALPFLGLIPPLVKLTTLGFPIPSVSHFQILFFTMIGPSGQFILEAFPSMLHPAISYLESCGLVPRLPPPVAQQLQTMGILRKRSAFSLKTSLSTVKWPSWKITALISALPAALSVFLKTISPLSLQSLHDGYNAHLHPSPFNLSWSLETFHVQSPSPFLPLSLTPPSPSEEIALPPPIFRVPPPLPAQETPSPPAPALVPPTQPPQPQPWEEIISTFLSSLNASSHKPSPSSAPLESPSPIPESFEVLAEAPQHQRSINECGALNQLPLPVPSPLQNSTNPKPPFPSSDLLSDMSCTGPVVEFETIFPAEYHMSNGSFPTRLRGHPRSSAPFPQKHCLLTAVASQLSYTEHQLWEFLCDMLPDSLLTNSEVENFGLSTDHLTCLSYRLHFECIIHTSHSTIPYGIKKASTVIQISYIDGPPKHFKAFIKLAAAAPGSNPSKSNLVRAALRFKYNDAFLPFWDAHQHTISVPHAKNLISNMKNGFDGITSQLSGPSNKSPKMKLLELDATIDVSFPRKCDVIHIAGFPGCGKSHPIQKLLQTPAFRHFRLSVPTNELRSEWKRDLNLPESEVWRLCTWETALFKSSNILVVDEIYKLPRGYLDLILLADPSIQLVIMLGDPLQGEYHSSHPSSSNSRLESETTRLSKYIDCYCWWTYRCPKAVADLFGVKTFNSNEGFIRAVLSHPPNLPNLVNSIATANTMQSLGHHALTISSSQGMTYSDPVTVLLDRHSLLITPQTALVALTRSRSGIYFIGSMYTASGSAGTSYMFSCALTGLPVDMMSAFPLFHTLPLIHEPIRSRRHRLVAGHTPSLHVPPSNKWPHRLHLPPHIPTSHSKDVILAHGIVASNAPERRLTTLHLPPTRLPLHFDLESCNPSTVSTSSTSNSEVPFTHAFLGESFEELAAHFLPAHDPDLKEVTVGDQTSQQFPYLDQPYTLSCQPSSLLAASHKPASDPTLLIFSISKRLRFRASSSPYAFTPNDLILGHLLYTNWCKAFGRCPNSTIPFNPALFAECICLNEYAQLSSKTQATIVSNASRSDPDWRYTVVRIFAKSQHKVNDGSIFGSWKACQTLALMHDFVILTLGPVKKYQRIIDHYDRPNFIYTHCGKTPSELSAWSHSFLKGDAYICNDYTSFDQSQHGEAVIFESLKMHRVGIPRHLIDLHIYLKTNVSTQFGPLTCMRLTGEPGTYDDNTDYNLAVIFSQYVISDHPIMVSGDDSVICGHPPINPNWPAVEKLLHLRFKTEETSLPLFCGYYVGPTGCCRNPFALFAKLMISYDKGNLFETLPSYLYEFSIGHRLGDVVRLLFPDHLLKYYSACWDLFCRKCTASQKLILSFEPIPPSFFSKLASTSRWVSKVLFSDLPTKIRDMLISSSKLPSYHQDPRVQYLESELLTSFNHGRLSTN |
| Enzyme Length | 1776 |
| Uniprot Accession Number | P20127 |
| Absorption | |
| Active Site | ACT_SITE 711; /note=For protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01074; ACT_SITE 797; /note=For protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01074 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539}; |
| DNA Binding | |
| EC Number | 2.7.7.48 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 899..906; /note=ATP; /evidence=ECO:0000250 |
| Features | Active site (2); Chain (1); Compositional bias (1); Domain (5); Nucleotide binding (1); Region (2) |
| Keywords | ATP-binding;Hydrolase;Nucleotide-binding;Nucleotidyltransferase;Protease;RNA-directed RNA polymerase;Thiol protease;Transferase;Viral RNA replication |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 198,129 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:21248 |
| Cross Reference Brenda |