| IED ID | IndEnz0002008088 |
| Enzyme Type ID | protease008088 |
| Protein Name |
Macrodontain-1 EC 3.4.22.- Macrodontain I |
| Gene Name | |
| Organism | Ananas macrodontes (False pineapple) (Pseudananas macrodontes) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Bromeliaceae Bromelioideae Ananas Ananas macrodontes (False pineapple) (Pseudananas macrodontes) |
| Enzyme Sequence | AVPQSIDWRDYGAVNEVKNQGPCGGCWAFAAIATVEGIYKIRKGNLVYLSEQEVLDCAVSYGCKGGWVNRAYDFIISNNGVTTDENYPYRAYQGTCNANYFPNSAYITGYSYVRRNDESHMMYAVSNQPIAALIDASGDNFQYYKGGVYSGPCGFSLNHAITIIGYGRDSYWIVRNSWGSSWGQGGYVRIRRDVSHSGGVCGIAMSPLFPTLQ |
| Enzyme Length | 213 |
| Uniprot Accession Number | P83443 |
| Absorption | |
| Active Site | ACT_SITE 26; /evidence=ECO:0000255|PROSITE-ProRule:PRU10088; ACT_SITE 159; /evidence=ECO:0000255|PROSITE-ProRule:PRU10089; ACT_SITE 176; /evidence=ECO:0000255|PROSITE-ProRule:PRU10090 |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by the general cysteine protease inhibitor E-64 (L-trans-epoxysuccinyl-leucylamide-(4-guanido)-butane). {ECO:0000269|PubMed:10686143, ECO:0000269|PubMed:11517945}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.22.- |
| Enzyme Function | FUNCTION: Cysteine protease that catalyzes the preferential cleavage: Ala-|-Xaa > Gln-|-Xaa > Tyr-Xaa >> Leu-|-Xaa > Gly-|-Xaa (PubMed:10686143, PubMed:11517945). Hydrolyzes the synthetic peptide substrate Bz-Phe-Val-Arg-pNA (PubMed:11517945). {ECO:0000269|PubMed:10686143, ECO:0000269|PubMed:11517945}. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 6.1-8.5. {ECO:0000269|PubMed:10686143, ECO:0000269|PubMed:11517945}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (3) |
| Keywords | Direct protein sequencing;Disulfide bond;Hydrolase;Protease;Thiol protease |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 23,486 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=13.4 uM for Bz-Phe-Val-Arg-pNA {ECO:0000269|PubMed:11517945}; |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |