| IED ID | IndEnz0002008149 |
| Enzyme Type ID | protease008149 |
| Protein Name |
Neutral protease 2 homolog AFUB_070680 EC 3.4.24.39 Deuterolysin AFUB_070680 |
| Gene Name | AFUA_4G02700 |
| Organism | Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Fumigati Neosartorya fumigata (Aspergillus fumigatus) Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus) |
| Enzyme Sequence | MKITALASAILAVAQGALALPARAPALDITLSQVNNTRIKAVVKNSGTEKITFVHLNFFNDPSPVKKVSLYRNATEVEFTGIKQRLRSDGLSNDALTTLAPGATYEDEFDIASTANLTQGGPVTVRTQGFVPIAMNNKIAGYIPYSSNELELEVDAEKAVAVPASIKPLDRRTKITSSCTGNRATVLNTALRNAASIASKAADAASSGSSALFTEYFKSTSGNIRSAVAARLKAVASEASMNGGGSTTYYCSDPYGYCDSNVLAYTLPSTNEVVNCELFYTLQEVTNDCHGQDQATTIIHEFTHAPGVYPPGTEDLGYGYSAATALSTSNALNNADSYALFANAVYLNCQGQTGGQTTWDGYSQPGQTEPGTQTMWDGYSQPGQTEPGTQTMWDGYSQPGQTEPGTQTTWDGYSQPGQIEPCTQTMWDGGSEPGQTEPDAQTMWDNFYQA |
| Enzyme Length | 450 |
| Uniprot Accession Number | Q4WA45 |
| Absorption | |
| Active Site | ACT_SITE 301; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Preferential cleavage of bonds with hydrophobic residues in P1'. Also 3-Asn-|-Gln-4 and 8-Gly-|-Ser-9 bonds in insulin B chain.; EC=3.4.24.39; |
| DNA Binding | |
| EC Number | 3.4.24.39 |
| Enzyme Function | FUNCTION: Secreted metalloproteinase that allows assimilation of proteinaceous substrates. Shows high activities on basic nuclear substrates such as histone and protamine. May be involved in virulence (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Disulfide bond (2); Erroneous initiation (1); Metal binding (3); Propeptide (1); Region (1); Signal peptide (1) |
| Keywords | Cleavage on pair of basic residues;Disulfide bond;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Secreted;Signal;Virulence;Zinc;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..19; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 47,911 |
| Kinetics | |
| Metal Binding | METAL 300; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 304; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 315; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
| Rhea ID | |
| Cross Reference Brenda |