| IED ID | IndEnz0002008229 |
| Enzyme Type ID | protease008229 |
| Protein Name |
SAGA-associated factor 11 11 kDa SAGA-associated factor |
| Gene Name | SGF11 YPL047W |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Enzyme Sequence | MTEETITIDSISNGILNNLLTTLIQDIVARETTQQQLLKTRYPDLRSYYFDPNGSLDINGLQKQQESSQYIHCENCGRDVSANRLAAHLQRCLSRGARR |
| Enzyme Length | 99 |
| Uniprot Accession Number | Q03067 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Component of the transcription regulatory histone acetylation (HAT) complex SAGA. SAGA is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3, SPT8 and SPT20) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3 and TRA1), and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). SGF11 is involved in transcriptional regulation of a subset of SAGA-regulated genes. Within the SAGA complex, participates in a subcomplex with SUS1, SGF73 and UBP8 required for deubiquitination of H2B and for the maintenance of steady-state H3 methylation levels. It is required to recruit UBP8 and SUS1 into the SAGA complex. {ECO:0000269|PubMed:15657441, ECO:0000269|PubMed:15657442}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Beta strand (2); Chain (1); Helix (4); Mutagenesis (10); Turn (1); Zinc finger (1) |
| Keywords | 3D-structure;Activator;Chromatin regulator;Metal-binding;Nucleus;Reference proteome;Transcription;Transcription regulation;Zinc;Zinc-finger |
| Interact With | Q6WNK7 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Nucleus {ECO:0000255|HAMAP-Rule:MF_03047, ECO:0000269|PubMed:14562095}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | NMR spectroscopy (1); Electron microscopy (1); X-ray crystallography (11) |
| Cross Reference PDB | 2LO2; 3KIK; 3KJL; 3M99; 3MHH; 3MHS; 4FIP; 4FJC; 4FK5; 4WA6; 4ZUX; 6AQR; 6T9L; |
| Mapped Pubmed ID | 10629113; 11893486; 12151331; 12213658; 14660704; 16554755; 16568953; 17082210; 17118266; 17337012; 17566592; 17694076; 17786152; 18374642; 18719252; 19077548; 19226466; 19229139; 19239896; 19246994; 19330770; 19411170; 19536198; 19561118; 19756577; 19834536; 20363118; 20489023; 20621284; 20634802; 20699646; 20800707; 21074715; 21320604; 21490951; 21602906; 21685874; 21734642; 21954323; 21984211; 22084422; 22094418; 22178374; 22426530; 22567346; 22771944; 23057668; 23731471; 23762321; 23964287; 24021962; 24040048; 24040173; 24136112; 24509845; 25043177; 25171917; 25216679; 25228644; 25441028; 25509853; 25526805; 25802992; 26051071; 26100014; 26456335; 26912860; 27057639; 27112564; 30150323; 31969703; |
| Motif | |
| Gene Encoded By | |
| Mass | 11,285 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |