| IED ID | IndEnz0002008231 |
| Enzyme Type ID | protease008231 |
| Protein Name |
E3 SUMO-protein ligase SIZ1 EC 2.3.2.- E3 SUMO-protein transferase SIZ2 SAP and Miz-finger domain-containing protein 1 Ubiquitin-like protein ligase 1 |
| Gene Name | SIZ1 ULL1 YDR409W |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Enzyme Sequence | MINLEDYWEDETPGPDREPTNELRNEVEETITLMELLKVSELKDICRSVSFPVSGRKAVLQDLIRNFLQNALVVGKSDPYRVQAVKFLIERIRKNEPLPVYKDLWNALRKGTPLSAITVRSMEGPPTVQQQSPSVIRQSPTQRRKTSTTSSTSRAPPPTNPDASSSSSSFAVPTIHFKESPFYKIQRLIPELVMNVEVTGGRGMCSAKFKLSKADYNLLSNPNSKHRLYLFSGMINPLGSRGNEPIQFPFPNELRCNNVQIKDNIRGFKSKPGTAKPADLTPHLKPYTQQNNVELIYAFTTKEYKLFGYIVEMITPEQLLEKVLQHPKIIKQATLLYLKKTLREDEEMGLTTTSTIMSLQCPISYTRMKYPSKSINCKHLQCFDALWFLHSQLQIPTWQCPVCQIDIALENLAISEFVDDILQNCQKNVEQVELTSDGKWTAILEDDDDSDSDSNDGSRSPEKGTSVSDHHCSSSHPSEPIIINLDSDDDEPNGNNPHVTNNHDDSNRHSNDNNNNSIKNNDSHNKNNNNNNNNNNNNNDNNNSIENNDSNSNNKHDHGSRSNTPSHNHTKNLMNDNDDDDDDRLMAEITSNHLKSTNTDILTEKGSSAPSRTLDPKSYNIVASETTTPVTNRVIPEYLGNSSSYIGKQLPNILGKTPLNVTAVDNSSHLISPDVSVSSPTPRNTASNASSSALSTPPLIRMSSLDPRGSTVPDKTIRPPINSNSYTASISDSFVQPQESSVFPPREQNMDMSFPSTVNSRFNDPRLNTTRFPDSTLRGATILSNNGLDQRNNSLPTTEAITRNDVGRQNSTPVLPTLPQNVPIRTNSNKSGLPLINNENSVPNPPNTATIPLQKSRLIVNPFIPRRPYSNVLPQKRQLSNTSSTSPIMGTWKTQDYGKKYNSG |
| Enzyme Length | 904 |
| Uniprot Accession Number | Q04195 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 2.3.2.- |
| Enzyme Function | FUNCTION: Acts as an E3 ligase mediating SUMO/Smt3 attachment to septins and PCNA. May be involved in chromosome maintenance. {ECO:0000269|PubMed:11333221, ECO:0000269|PubMed:11572779, ECO:0000269|PubMed:11577116, ECO:0000269|PubMed:11587849, ECO:0000269|PubMed:12226657}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Protein modification; protein sumoylation. |
| nucleotide Binding | |
| Features | Beta strand (16); Chain (1); Compositional bias (4); Domain (2); Helix (15); Metal binding (4); Modified residue (2); Mutagenesis (4); Region (7); Turn (2); Zinc finger (1) |
| Keywords | 3D-structure;Cytoplasm;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Transferase;Ubl conjugation;Ubl conjugation pathway;Zinc;Zinc-finger |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16109721}. Nucleus {ECO:0000269|PubMed:11572779, ECO:0000269|PubMed:16109721}. Bud neck {ECO:0000269|PubMed:11572779, ECO:0000269|PubMed:11587849, ECO:0000269|PubMed:12761287, ECO:0000269|PubMed:16109721}. Note=Present at the bud neck in early M-phase. {ECO:0000269|PubMed:11587849}. |
| Modified Residue | MOD_RES 132; /note=Phosphoserine; /evidence=ECO:0007744|PubMed:18407956; MOD_RES 794; /note=Phosphoserine; /evidence=ECO:0007744|PubMed:18407956 |
| Post Translational Modification | PTM: Phosphorylated in early M-phase. {ECO:0000269|PubMed:11572779, ECO:0000269|PubMed:11587849}.; PTM: Autosumoylated upon ethanol stress. {ECO:0000269|PubMed:15166219}. |
| Signal Peptide | |
| Structure 3D | NMR spectroscopy (1); X-ray crystallography (2) |
| Cross Reference PDB | 2RNN; 3I2D; 5JNE; |
| Mapped Pubmed ID | 11595179; 11743162; 12429940; 14607092; 15781853; 15870299; 16095633; 16204216; 16449653; 16554755; 16882975; 17031663; 17077124; 17081974; 17403926; 17664284; 17689491; 17728242; 17888002; 18031227; 18155241; 18583943; 18585101; 18701921; 18831036; 18846224; 19107407; 19107412; 19139279; 19217833; 19230795; 19384408; 19482523; 19536198; 19635810; 19709431; 19748360; 19841731; 20351217; 20463880; 20620062; 20847899; 21151918; 21222284; 21362066; 21536034; 21666682; 21743457; 21892772; 22020070; 22419078; 22562154; 22705796; 22732495; 22771040; 23034805; 23122649; 23395907; 23443663; 23499004; 23547164; 23550137; 23571759; 23712011; 23812602; 23935535; 24196836; 24300101; 24395824; 24485833; 24498309; 24500206; 24765138; 24904688; 25434491; 25795125; 26083678; 26113493; 26253534; 26598615; 26783300; 26800527; 26861880; 26921322; 26953525; 26960795; 27056668; 27130983; 27134164; 27134171; 27215325; 27373152; 27384026; 27505077; 27509863; 27664980; 27683094; 27693354; 27839712; 33929514; |
| Motif | |
| Gene Encoded By | |
| Mass | 100,796 |
| Kinetics | |
| Metal Binding | METAL 377; /note=Zinc; /evidence=ECO:0000255|PROSITE-ProRule:PRU00452; METAL 379; /note=Zinc; /evidence=ECO:0000255|PROSITE-ProRule:PRU00452; METAL 400; /note=Zinc; /evidence=ECO:0000255|PROSITE-ProRule:PRU00452; METAL 403; /note=Zinc; /evidence=ECO:0000255|PROSITE-ProRule:PRU00452 |
| Rhea ID | |
| Cross Reference Brenda |