| IED ID |
IndEnz0002008244 |
| Enzyme Type ID |
protease008244 |
| Protein Name |
HTH-type transcriptional regulator SinR
|
| Gene Name |
sinR flaD sin BSU24610 |
| Organism |
Bacillus subtilis (strain 168) |
| Taxonomic Lineage |
cellular organisms
Bacteria
Terrabacteria group
Firmicutes
Bacilli
Bacillales
Bacillaceae
Bacillus
Bacillus subtilis group
Bacillus subtilis
Bacillus subtilis subsp. subtilis
Bacillus subtilis (strain 168)
|
| Enzyme Sequence |
MIGQRIKQYRKEKGYSLSELAEKAGVAKSYLSSIERNLQTNPSIQFLEKVSAVLDVSVHTLLDEKHETEYDGQLDSEWEKLVRDAMTSGVSKKQFREFLDYQKWRKSQKEE |
| Enzyme Length |
111 |
| Uniprot Accession Number |
P06533 |
| Absorption |
|
| Active Site |
|
| Activity Regulation |
|
| Binding Site |
|
| Calcium Binding |
|
| catalytic Activity |
|
| DNA Binding |
DNA_BIND 17..36; /note=H-T-H motif; /evidence=ECO:0000255|PROSITE-ProRule:PRU00257 |
| EC Number |
|
| Enzyme Function |
FUNCTION: Negative as well as positive regulator of alternate developmental processes that are induced at the end of vegetative growth in response to nutrient depletion. Binds to the alkaline protease (aprE) gene at two sites. Also acts as a repressor of the key sporulation gene spo0A. Negatively regulates transcription of the eps operon, which is responsible for the biosynthesis of an exopolysaccharide involved in biofilm formation; therefore it could govern the transition between a state in which bacteria swim or swarm and a state in which bacteria assemble into multicellular communities. Acts with Hpr as a corepressor of epr expression. Also negatively regulates transcription of the lutABC operon, which is required for lactate utilization. Repressor activity is regulated by SinI. {ECO:0000269|PubMed:15661000, ECO:0000269|PubMed:16923912, ECO:0000269|PubMed:1898931, ECO:0000269|PubMed:7642487}. |
| Temperature Dependency |
|
| PH Dependency |
|
| Pathway |
|
| nucleotide Binding |
|
| Features |
Beta strand (1); Chain (1); DNA binding (1); Domain (2); Helix (7) |
| Keywords |
3D-structure;Activator;DNA-binding;Reference proteome;Repressor;Sporulation;Transcription;Transcription regulation |
| Interact With |
|
| Induction |
|
| Subcellular Location |
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| Modified Residue |
|
| Post Translational Modification |
|
| Signal Peptide |
|
| Structure 3D |
NMR spectroscopy (2); X-ray crystallography (4) |
| Cross Reference PDB |
1B0N;
2YAL;
3QQ6;
3ZKC;
5TN0;
5TN2;
|
| Mapped Pubmed ID |
21708175;
23430750;
31493408;
|
| Motif |
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| Gene Encoded By |
|
| Mass |
12,989 |
| Kinetics |
|
| Metal Binding |
|
| Rhea ID |
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| Cross Reference Brenda |
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