| IED ID | IndEnz0002008256 |
| Enzyme Type ID | protease008256 |
| Protein Name |
Probable alpha-L-glutamate ligase EC 6.3.2.- |
| Gene Name | rimK Spea_2101 |
| Organism | Shewanella pealeana (strain ATCC 700345 / ANG-SQ1) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Alteromonadales Shewanellaceae Shewanella Shewanella pealeana Shewanella pealeana (strain ATCC 700345 / ANG-SQ1) |
| Enzyme Sequence | MSDNKFIIGSEEWCAFPALGVPAIKARVDSGARTSSIHAVNIRPFKREGEPWVSFELHPIQNSRKIILRCESKVVDRRNIKSSNGESEKRYVIASVIQLGGNAWEVELTLTNRDSMGYRMLLGREAMSNKTLVDPSESFCLGEKGDLEVEQLYGVKTAKKSGLKIGLLASNPDLYSNRRIIEAGEQRGHEMVFLNISQCYLKLDASSPEVHYRGGRVLNNVDAVIPRIRPSMTFYGCALTRHFESLNIEALNTSDAITRSRDKLFSLQLLQKNNLDIPTSGFANSPVDTKDLIDMVGGAPLIVKLLEGTQGKGVVLAETTKAAESVINAFKSLRVNLLVQEFIKEAQGKDLRCFVIDGKIVASIERTAAPGEFRANIHMGGSASIVSVSAAEKQLAIKAAKTMGLRVAGVDIIRSSRGPLLLEINSSPGLEGIESATGIDIAGAMIESIEKKLGWKAD |
| Enzyme Length | 458 |
| Uniprot Accession Number | A8H4D4 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | BINDING 304; /note=ATP; /evidence=ECO:0000255|HAMAP-Rule:MF_01552; BINDING 350; /note=ATP; /evidence=ECO:0000255|HAMAP-Rule:MF_01552 |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 6.3.2.- |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 341..342; /note=ATP; /evidence=ECO:0000255|HAMAP-Rule:MF_01552; NP_BIND 374..376; /note=ATP; /evidence=ECO:0000255|HAMAP-Rule:MF_01552 |
| Features | Binding site (2); Chain (1); Domain (1); Metal binding (8); Nucleotide binding (2); Region (2) |
| Keywords | ATP-binding;Ligase;Magnesium;Manganese;Metal-binding;Nucleotide-binding;Protein biosynthesis;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 49,927 |
| Kinetics | |
| Metal Binding | METAL 411; /note=Magnesium 1; /evidence=ECO:0000255|HAMAP-Rule:MF_01552; METAL 411; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_01552; METAL 423; /note=Magnesium 1; /evidence=ECO:0000255|HAMAP-Rule:MF_01552; METAL 423; /note=Magnesium 2; /evidence=ECO:0000255|HAMAP-Rule:MF_01552; METAL 423; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_01552; METAL 423; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_01552; METAL 425; /note=Magnesium 2; /evidence=ECO:0000255|HAMAP-Rule:MF_01552; METAL 425; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_01552 |
| Rhea ID | |
| Cross Reference Brenda |