| IED ID | IndEnz0002008280 |
| Enzyme Type ID | protease008280 |
| Protein Name |
Subtilisin-like protease 3 SbtM3 Subtilase 3 EC 3.4.21.- |
| Gene Name | SBTM3 |
| Organism | Lotus japonicus (Lotus corniculatus var. japonicus) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids fabids Fabales Fabaceae Papilionoideae 50 kb inversion clade NPAAA clade Hologalegina robinioid clade Loteae Lotus Lotus japonicus (Lotus corniculatus var. japonicus) |
| Enzyme Sequence | MTKVVQFSLLLALILVLSVSLASAASQNLEFTELEDEDQSNLSTYIVHVRKPQVIQSDDLHTFYYSLLPESTKTTNQRIVFTYRNVVNGFAVKLTPEEAKALQQNEEVVSARPEKILSLHTTHTPSFLGLQQGLGLWKGSNSGKGVIIGILDTGISPFHPSFSDEGMPSPPAKWNGICEFTGKRTCNNKIIGARNFVKTKNLTLPFDDVGHGTHTASTAAGRPVQGANVYGNANGTAVGMAPDAHIAMYKVCGLVGCSESAILAGMDTAVDDGVDVLSLSLGGPSGPFFEDPIALGAFGAIQKGIFVSCSAANSGPAYSSLSNEAPWILTVGASSIDRTIMATAKLGNGKEYVGQSVFQPKDFAPSLLPLVYAGANGNNNFSVFCAPESLNRSDVEGKVVLCEDGGFVPRVFKGKAVKDAGGAAMILMNSVLEDFNPIADVHVLPAVHISYEAGLALKEYINSTSTPTATILFEGTVIGNLLAPQVTSFSSRGPSKASPGILKPDIIGPGLNILAAWPVSLDNSTTPPFNIISGTSMSCPHLSGIAALLKNSHPDWSPAAIKSAIMTTASQVNLGGTPILDQRLVPADVFATGAGHVNPVKANDPGLVYDIEPNDYIPYLCGLNYTDREVGVILQQRVRCSEVNHIAEAELNYPSFSILLGNTTQLYTRTVANVGPANSTYTAEIGVPVGVGMSLSPAQLTFTEVGQKLTYSVSFIPFSEDRDNHTFAQGSLKWVSGKYSVRSPISFIFL |
| Enzyme Length | 750 |
| Uniprot Accession Number | A9QY39 |
| Absorption | |
| Active Site | ACT_SITE 152; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240; ACT_SITE 211; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240; ACT_SITE 536; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Required for arbuscular mycorrhiza (AM) development during AM symbiosis with AM fungi (e.g. Glomeromycota intraradices). {ECO:0000269|PubMed:19220794}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Domain (3); Glycosylation (11); Signal peptide (1) |
| Keywords | Apoplast;Glycoprotein;Hydrolase;Protease;Secreted;Serine protease;Signal |
| Interact With | |
| Induction | INDUCTION: Rapidly induced in roots during development of arbuscular mycorrhiza (AM) upon colonization by AM fungus (e.g. Glomeromycota intraradices). {ECO:0000269|PubMed:19220794}. |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast {ECO:0000250|UniProtKB:A9QY40}. Note=Accumulates in the intercellular spaces and the periarbuscular space (PAS) during arbuscular mycorrhizal (AM) symbiosis. {ECO:0000250|UniProtKB:A9QY40}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..24; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 79,452 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |