| IED ID | IndEnz0002008281 |
| Enzyme Type ID | protease008281 |
| Protein Name |
Capsid assembly protease C EC 3.4.21.- Gene product C GPC Minor capsid protein C |
| Gene Name | C; NU3 lambdap05 |
| Organism | Escherichia phage lambda (Bacteriophage lambda) |
| Taxonomic Lineage | Viruses Duplodnaviria Heunggongvirae Uroviricota Caudoviricetes Caudovirales Siphoviridae (phages with long non-contractile tails) Lambdavirus Escherichia phage lambda (Bacteriophage lambda) |
| Enzyme Sequence | MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISSLTDAVSGDSLTAQEALATLALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYSGMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALANDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHKVDGNPYSHLPDDVRETLQSRMDATRQMFAQKVSAYTGLSVQVVLDTEAAVYSGQEAIDAGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTDVVPATEGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVKTARRILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV |
| Enzyme Length | 439 |
| Uniprot Accession Number | P03711 |
| Absorption | |
| Active Site | ACT_SITE 166; /note=Nucleophile; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Assembly protease promotes icosahedral procapsid assembly. Autocatalytic cleavage may release the capsid scaffolding protein. The protease domain catalyzes the cleavage of the capsid scaffolding protein after complete procapsid formation. Assembly protease and cleavages products are evicted from the capsid before or during DNA packaging.; FUNCTION: Scaffolding protein Nu3 promotes icosahedral procapsid assembly. Acts by binding the major capsid protein gpE and multimerizing in interaction with portal dodecamer, thereby placing gpE in a context facilitating icosahedral procapsid formation. Cleaved by capsid assembly protease C after capsid completion. The cleavages products are evicted from the capsid before or during DNA packaging. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Alternative sequence (1); Chain (1); Compositional bias (1); Erroneous initiation (1); Mutagenesis (1); Region (1) |
| Keywords | Alternative initiation;Capsid protein;Host cytoplasm;Hydrolase;Protease;Reference proteome;Serine protease;Viral capsid assembly;Viral capsid maturation;Viral release from host cell;Virion |
| Interact With | Itself |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: [Isoform Capsid assembly protease C]: Virion. Host cytoplasm.; SUBCELLULAR LOCATION: [Isoform Capsid scaffolding protein Nu3]: Host cytoplasm. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 21943085; |
| Motif | |
| Gene Encoded By | |
| Mass | 45,940 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |