| IED ID | IndEnz0002008284 |
| Enzyme Type ID | protease008284 |
| Protein Name |
Extracellular small neutral protease EC 3.4.24.77 SCNP Snapalysin |
| Gene Name | snpA |
| Organism | Streptomyces caespitosus |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Streptomycetales Streptomycetaceae Streptomyces Streptomyces caespitosus |
| Enzyme Sequence | TVTVTYDPSNAPSFQQEIANAAQIWNSSVRNVQLRAGGNADFSYYEGNDSRGSYAQTDGHGRGYIFLDYQQNQQYDSTRVTAHETGHVLGLPDHYQGPCSELMSGGGPGPSCTNPYPNAQERSRVNALWANG |
| Enzyme Length | 132 |
| Uniprot Accession Number | P56406 |
| Absorption | |
| Active Site | ACT_SITE 84 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolyzes proteins with a preference for Tyr or Phe in the P1' position. Has no action on amino-acid p-nitroanilides.; EC=3.4.24.77; |
| DNA Binding | |
| EC Number | 3.4.24.77 |
| Enzyme Function | FUNCTION: Specifically hydrolyzes the peptide bond at the imino side of aromatic residues. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (6); Chain (1); Disulfide bond (1); Helix (6); Metal binding (5); Turn (2) |
| Keywords | 3D-structure;Calcium;Direct protein sequencing;Disulfide bond;Hydrolase;Metal-binding;Metalloprotease;Protease;Secreted;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (3) |
| Cross Reference PDB | 1C7K; 1KUH; 4HX3; |
| Mapped Pubmed ID | 11132632; |
| Motif | |
| Gene Encoded By | |
| Mass | 14,376 |
| Kinetics | |
| Metal Binding | METAL 76; /note=Calcium; /evidence=ECO:0000269|PubMed:9089404; METAL 78; /note=Calcium; /evidence=ECO:0000269|PubMed:9089404; METAL 83; /note=Zinc; catalytic; /evidence=ECO:0000269|PubMed:9089404; METAL 87; /note=Zinc; catalytic; /evidence=ECO:0000269|PubMed:9089404; METAL 93; /note=Zinc; catalytic; /evidence=ECO:0000269|PubMed:9089404 |
| Rhea ID | |
| Cross Reference Brenda |