| IED ID | IndEnz0002008286 |
| Enzyme Type ID | protease008286 |
| Protein Name |
Serine protease 7 EC 3.4.21.- Melanization protease 2 |
| Gene Name | Sp7 MP2 PAE1 CG3066 |
| Organism | Drosophila melanogaster (Fruit fly) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group melanogaster subgroup Drosophila melanogaster (Fruit fly) |
| Enzyme Sequence | MKSTRKVVGIFLATCLLPFTVLQNVAAQGSCRNPNQKQGQCLSIYDCQSLLSVIQQSYVSPEDRTFLRNSQCLDGVGRQPYVCCTSDRSFGSQEATSAAPPPTTTSSSSRGQDGQAGLGNLLPSPPKCGPHSFSNKVYNGNDTAIDEFNWMALLEYVDNRGRRELSCGGSLINNRYVLTAAHCVIGAVETEVGHLTTVRLGEYDTSKDVDCIDDICNQPILQLGIEQATVHPQYDPANKNRIHDIALLRLDRPVVLNEYIQPVCLPLVSTRMAINTGELLVVSGWGRTTTARKSTIKQRLDLPVNDHDYCARKFATRNIHLISSQLCVGGEFYRDSCDGDSGGPLMRRGFDQAWYQEGVVSFGNRCGLEGWPGVYTRVADYMDWIVETIRP |
| Enzyme Length | 391 |
| Uniprot Accession Number | Q9V3Z2 |
| Absorption | |
| Active Site | ACT_SITE 182; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU00274; ACT_SITE 244; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU00274; ACT_SITE 341; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU00274 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Serine protease which, by cleaving and activating prophenoloxidase (PPO1) after immune challenge, plays an essential role in the melanization immune response to septic wounding (PubMed:16256951, PubMed:16322759, PubMed:19071960, PubMed:24260243, PubMed:16861233). May function in diverse Hayan-dependent PPO1-activating cascades that are negatively controlled by different serpin proteins; Spn27A in the hemolymph and Spn77BA in the trachea (PubMed:18854145, PubMed:24260243, PubMed:16322759). Important for the innate immune response to fungi (PubMed:16861233). Regulation of melanization and PPO1 activation appears to be largely independent of the Toll signaling pathway (PubMed:16861233). {ECO:0000269|PubMed:16256951, ECO:0000269|PubMed:16322759, ECO:0000269|PubMed:16861233, ECO:0000269|PubMed:18854145, ECO:0000269|PubMed:19071960, ECO:0000269|PubMed:24260243}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Compositional bias (1); Disulfide bond (8); Domain (2); Glycosylation (1); Metal binding (4); Propeptide (1); Region (1); Signal peptide (1) |
| Keywords | Calcium;Disulfide bond;Glycoprotein;Hydrolase;Metal-binding;Protease;Reference proteome;Secreted;Serine protease;Signal;Zymogen |
| Interact With | |
| Induction | INDUCTION: Up-regulated after wounding. Levels are higher with septic wounding, using either Gram-negative or Gram-positive bacteria. {ECO:0000269|PubMed:16256951}. |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:24260243}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..27; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11606746; 12032070; 12364793; 12568721; 15466469; 16277749; 16399077; 16996061; 17356067; 17588928; 17996400; 18801354; 19129115; 19378452; 19546158; 19851448; 19939842; 20220848; 20865166; 21074052; 21940639; 21962711; 22368770; 22724070; 23071443; 23255357; 23401590; 23583758; 23840627; 23913921; 24077308; 24281154; 24409421; 24443439; 25092914; 25180232; 25267525; 25688716; 26162375; 26437768; 26453810; 26509186; 26607310; 27280707; 27628033; 28449654; 28450373; 30367934; 30733377; 31018123; 31068592; 31963772; 32656090; 33827210; 33914801; |
| Motif | |
| Gene Encoded By | |
| Mass | 43,128 |
| Kinetics | |
| Metal Binding | METAL 202; /note=Calcium; /evidence=ECO:0000250|UniProtKB:O97366; METAL 204; /note=Calcium; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:O97366; METAL 207; /note=Calcium; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:O97366; METAL 210; /note=Calcium; /evidence=ECO:0000250|UniProtKB:O97366 |
| Rhea ID | |
| Cross Reference Brenda |