| IED ID | IndEnz0002008314 |
| Enzyme Type ID | protease008314 |
| Protein Name |
Neprilysin-1 EC 3.4.24.11 |
| Gene Name | Nep1 CG5905 |
| Organism | Drosophila melanogaster (Fruit fly) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group melanogaster subgroup Drosophila melanogaster (Fruit fly) |
| Enzyme Sequence | MSQQHEATAAAAEKPLNNGYLQANAPLEELSATVVSPLLGQQQVQHQAPHQMQQQQQQQQQNKLPTVVFLAPDGSGGVGIQRGNPAQGNPGMVTGTGSHSDWLLKESQQRRRLLVLAIAFTVLGAAIGALAIYFASVHQRCHLYRLEPDNDDRPNGRWNQDSGSAHEGQDNICMTQECVRTAASLLSAMDLNSDPCEDFFQYACGTWNKMHPIPEDRSSISTFEVLSDQQQVILRAVLEEPIDERDNKATIKAKTFFKSCMDIPQIRKIGTGRLKQVLQSLGGWPVIERNWSPPADLSVERLMGQLRLNYSEPVMIELYVGADDKNSSVNILQMDQLQYALPSRDYYLKESSANDRRAYHRYMTQVALLLGADPATAAAELEKVVLFETQLVNVSLPEADRHDTSLVYRKMLLPELQELVPEVQWQEYLQAALGPGIPLQEDEPLVTYGLHYLTEMGKILAHTDRRVVHNYMLWRLVMSLMSHMIDEYQRERVEFRKILMGIQSERTRWSQCVEWTNKKLGVAVGALFIRDNFNQESKEVALEMIHTIRAAFNELLAENDWMDDETRAVAKEKADSMNERIGYPELLTNATELEQEYVNLTIVPDNFINNVLSILQWESEKMLRLLRQPVDKEKWTTEPAVVNAFYNPNKNDIVFPAGILQPLFYSQHFPKSLNYGGIGVVIGHEITHGFDDKGRQFDKEGNMMQWWNNATIEAFRERTQCVIDQYSRYKINEVDMFMDGRMTQGENIADNGGLKQAFRAYKKWETLHGREQQLPGLNMTHDQLFFLNYAQIWCGSMRPEDALTKIRSAVHSPGFVRVLGPLSNSRDFASAYKCPLGSTMNPAEKCSVW |
| Enzyme Length | 849 |
| Uniprot Accession Number | Q9W436 |
| Absorption | |
| Active Site | ACT_SITE 685; /evidence="ECO:0000255|PROSITE-ProRule:PRU01233, ECO:0000255|PROSITE-ProRule:PRU10095"; ACT_SITE 750; /note="Proton donor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU01233" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Preferential cleavage of polypeptides between hydrophobic residues, particularly with Phe or Tyr at P1'.; EC=3.4.24.11; Evidence={ECO:0000250|UniProtKB:Q8T062}; |
| DNA Binding | |
| EC Number | 3.4.24.11 |
| Enzyme Function | FUNCTION: Metalloendoprotease which functions in fertility and memory formation (PubMed:24395329, PubMed:27629706). Required in the dorsal paired medial neurons and alpha/beta mushroom body neurons for the proper formation of long-term and middle-term memories (PubMed:27629706). Required in males to maximise egg-laying in female mates and is also required in females for their fertility (PubMed:24395329). {ECO:0000269|PubMed:24395329, ECO:0000269|PubMed:27629706}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Disulfide bond (5); Domain (1); Glycosylation (7); Metal binding (3); Region (2); Topological domain (2); Transmembrane (1) |
| Keywords | Cell membrane;Disulfide bond;Glycoprotein;Hydrolase;Membrane;Metal-binding;Metalloprotease;Protease;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass type II membrane protein {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10961941; 11223883; 12505997; 16971484; 17028348; 18215274; 18463098; 18791638; 20220848; 20371351; 21074052; 21593416; 21684629; 22384345; 22583317; 23071443; 23827522; 24952591; 25922346; 26772998; 28213160; 28633019; 28931572; 29951817; 30246928; 31068592; 31722958; 31746739; 32019113; 32303647; 33049996; 33812867; 34189422; |
| Motif | |
| Gene Encoded By | |
| Mass | 96,534 |
| Kinetics | |
| Metal Binding | METAL 684; /note="Zinc; catalytic"; /evidence="ECO:0000255|PROSITE-ProRule:PRU01233, ECO:0000255|PROSITE-ProRule:PRU10095"; METAL 688; /note="Zinc; catalytic"; /evidence="ECO:0000255|PROSITE-ProRule:PRU01233, ECO:0000255|PROSITE-ProRule:PRU10095"; METAL 746; /note="Zinc; catalytic"; /evidence="ECO:0000255|PROSITE-ProRule:PRU01233" |
| Rhea ID | |
| Cross Reference Brenda |