| IED ID | IndEnz0002008318 |
| Enzyme Type ID | protease008318 |
| Protein Name |
Mitochondrial intermediate peptidase 2 MIP 2 EC 3.4.24.59 Octapeptidyl aminopeptidase 2 |
| Gene Name | OCT2 CNB02140 |
| Organism | Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Basidiomycota Agaricomycotina Tremellomycetes Tremellales (jelly fungi) Cryptococcaceae Cryptococcus Cryptococcus neoformans species complex Cryptococcus neoformans (Filobasidiella neoformans) Cryptococcus neoformans var. neoformans Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans) |
| Enzyme Sequence | MRRLQQSLRRRSARRCPFILIPHRLLTTSYASYKPAPQATLEIEDTNSPTFLLKTSPIQLPARATSDDSAIKAHFDLPHSIFGDMVGVRREHVKGLFHYDSLTQPESLMRLTDRTLIQASAIVQRIVVAPQDPTGRELRLVVKNLDRLSDILCGVIDMCELIRNVHPHQDWVNQSDRTHQILCSFMNELNATRGLYESLAKAIAHPFNDPLTTSELRVARIFLTDFERSGIHLPPSVRERFVKHSDALLFLGRSFLSSASSGPSTVPHIEIPDPHRLLTGLGRQFVDSLPRTGRNGQAVIEPGSWEAQMILRYAREGRARELVYVGGMRADKKRISVLEAMLKERAELASVLGKNNWAEVVLVDKMTKTPENVMRFLTSLAQHHQPVARAEVDMLRRMKATALTGNYFDPRNSRTRHLPLFHAWDRDYYSDKYLTSLIPTGSPPSISPYLSTGTVMSGLSRIFSRLYGISFKPAVVSPGEVWHPSVRRLDVVHEEEGLIGVIYCDFFSRIGKSSGAAHYTVRCSRRVDDDDIDGDGLPEDWDKPYGPGLEADKESLSGKPGKYQLPIIALSMDVGTVNEGRPALLNWQELETLFHEMGHAIHSMIGRTEYHNVSGTRCATDFVELPSILMEHFVSSPEVLSTLAFHHATGEPLPIPVIEAHLALNQSLSALETHGQIAMALLDQKYHTLRHGQDSFDSTAIWFQLQQEIGVIQPVPGTAWQMQFGHLYGYGATYYSYLFDRAIAGKIWSTLFHRSGTSQAYDRKAEGILSREGGELLKEKVLKWGGGRDPWEMVGDVIGGVEGDELSKGDERALALVGSWSVV |
| Enzyme Length | 823 |
| Uniprot Accession Number | P0CQ20 |
| Absorption | |
| Active Site | ACT_SITE 596; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.; EC=3.4.24.59; |
| DNA Binding | |
| EC Number | 3.4.24.59 |
| Enzyme Function | FUNCTION: Cleaves proteins, imported into the mitochondrion, to their mature size. While most mitochondrial precursor proteins are processed to the mature form in one step by mitochondrial processing peptidase (MPP), the sequential cleavage by MIP of an octapeptide after initial processing by MPP is a required step for a subgroup of nuclear-encoded precursor proteins destined for the matrix or the inner membrane (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Erroneous initiation (1); Metal binding (3); Region (1); Transit peptide (1) |
| Keywords | Hydrolase;Metal-binding;Metalloprotease;Mitochondrion;Protease;Reference proteome;Transit peptide;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Mitochondrion matrix {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 92,172 |
| Kinetics | |
| Metal Binding | METAL 595; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 599; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 602; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
| Rhea ID | |
| Cross Reference Brenda |