| IED ID | IndEnz0002008353 |
| Enzyme Type ID | protease008353 |
| Protein Name |
Vacuolar membrane protease EC 3.4.-.- FXNA-related family protease 1 |
| Gene Name | Lema_P032730 |
| Organism | Leptosphaeria maculans (strain JN3 / isolate v23.1.3 / race Av1-4-5-6-7-8) (Blackleg fungus) (Phoma lingam) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta dothideomyceta Dothideomycetes Pleosporomycetidae Pleosporales Pleosporineae Leptosphaeriaceae Leptosphaeria Leptosphaeria maculans species complex Leptosphaeria maculans (Blackleg fungus) (Phoma lingam) Leptosphaeria maculans 'brassicae' group Leptosphaeria maculans (strain JN3 / isolate v23.1.3 / race Av1-4-5-6-7-8) (Blackleg fungus) (Phoma lingam) |
| Enzyme Sequence | MARYNPLAFTSGPVVFFITITYTALLIALLLTHLTLPSYPSHPPLGINLTQAWSDLEHITREFHPYNSHANDNVRAYLLSRIEHMMASKKLGSDQVQIIDDNISNATFSSGNTTVYFEGTNLIVAVRGSHDDQAFNDRNRRPDNGGVLVNAHYDSVSSGYGATDDGVGVVSVLQLLSFFTEPKNWPKRTVVLLLNNGEEDFLNGAKAFMRHDISQVPHTFVNLEGAGAGGRAAMFRSTDTHVTRFYRKSEHPFGTVVSGDGFKKGLVRSETDYKVFFEELGLAGLDIAFIEPRAKYHTIEDSTRETSLNSVWHMLSAAIATTSGLAADTSTPDRESHDDAVWFDIFGKVFIVFQLHTFFALCVTLLVVAPLTLIGLAWSLHKADRNYLFARKAFVYSADDDEPIHLYGWRGFFRFPIAFGIATSIVVGLAMMLSAWFAVSWFLLHGADAMRPSALQRMYSLLWLFIGSFCLLVFFTILANNHQVAAGYPSLFCFATVFLANVLSFLELFLAPPKSAYAWNVHQNADGGSRPLTSSATAARSDNRATTDDDATETTSLLSGNRRSFARHDAGRRDAINEGANSQEPESRRRLDLGQPHSGEQEWSGKLPSWIWIVQFSLLAPMIVILVGQIALLLTSALYQTPSDGNSPLYIYTSIAALAVFLVAPIGPFIHRFTHHVPTFLFLLCVATTIYNLVAFPFSEQHKLKVYFVQRVDCDTGVNTVSLTGLDGYVQRIAGNMPSAQQSSLNCSTPDIVTRKELRMCEWEGLHPNVVSPTSQYSNKTRATDWIDYEIFHDDSNNRGRR |
| Enzyme Length | 802 |
| Uniprot Accession Number | E4ZQC4 |
| Absorption | |
| Active Site | ACT_SITE 198; /note=Proton acceptor; /evidence=ECO:0000250|UniProtKB:P80561 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.-.- |
| Enzyme Function | FUNCTION: May be involved in vacuolar sorting and osmoregulation. {ECO:0000250|UniProtKB:P38244}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Compositional bias (1); Glycosylation (6); Metal binding (6); Region (2); Site (1); Topological domain (10); Transmembrane (9) |
| Keywords | Glycoprotein;Hydrolase;Membrane;Metal-binding;Metalloprotease;Protease;Reference proteome;Transmembrane;Transmembrane helix;Vacuole;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000250|UniProtKB:P38244}; Multi-pass membrane protein {ECO:0000255}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 89,157 |
| Kinetics | |
| Metal Binding | METAL 152; /note=Zinc 1; catalytic; /evidence=ECO:0000250|UniProtKB:P80561; METAL 164; /note=Zinc 1; catalytic; /evidence=ECO:0000250|UniProtKB:P80561; METAL 164; /note=Zinc 2; catalytic; /evidence=ECO:0000250|UniProtKB:P80561; METAL 199; /note=Zinc 2; catalytic; /evidence=ECO:0000250|UniProtKB:P80561; METAL 224; /note=Zinc 1; catalytic; /evidence=ECO:0000250|UniProtKB:P80561; METAL 297; /note=Zinc 2; catalytic; /evidence=ECO:0000250|UniProtKB:P80561 |
| Rhea ID | |
| Cross Reference Brenda |