| IED ID | IndEnz0002008361 |
| Enzyme Type ID | protease008361 |
| Protein Name |
Serralysin EC 3.4.24.40 Extracellular metalloproteinase Zinc proteinase |
| Gene Name | |
| Organism | Serratia marcescens |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Yersiniaceae Serratia Serratia marcescens |
| Enzyme Sequence | MQSTKKAIEITESSLAAATTGYDAVDDLLHYHERGNGIQINGKDSFSNEQAGLFITRENQTWNGYKVFGQPVKLTFSFPDYKFSSTNVAGDTGLSKFSAEQQQQAKLSLQSWADVANITFTEVAAGQKANITFGNYSQDRPGHYDYGTQAYAFLPNTIWQGQDLGGQTWYNVNQSNVKHPATEDYGRQTFTHEIGHALGLSHPGDYNAGEGNPTYNDVTYAEDTRQFSLMSYWSETNTGGDNGGHYAAAPLLDDIAAIQHLYGANPSTRTGDTVYGFNSNTGRDFLSTTSNSQKVIFAAWDAGGNDTFDFSGYTANQRINLNEKSFSDVGGLKGNVSIAAGVTIENAIGGSGNDVIVGNAANNVLKGGAGNDVLFGGGGADELWGGAGKDIFVFSAASDSAPGASDWIRDFQKGIDKIDLSFFNKEANSSDFIHFVDHFSGTAGEALLSYNASSNVTDLSVNIGGHQAPDFLVKIVGQVDVATDFIV |
| Enzyme Length | 487 |
| Uniprot Accession Number | P23694 |
| Absorption | |
| Active Site | ACT_SITE 193 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Preferential cleavage of bonds with hydrophobic residues in P1'.; EC=3.4.24.40; Evidence={ECO:0000269|PubMed:16391997}; |
| DNA Binding | |
| EC Number | 3.4.24.40 |
| Enzyme Function | FUNCTION: Has insecticidal activity against the locust M.palpalis. When administered orally to locusts at a low dose it causes them to lie on their sides exhibiting sporadic limb movements and muscular twitching, followed by full recovery. When administered at higher doses the same symptoms are observed, followed by death. {ECO:0000269|PubMed:16391997}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (27); Chain (1); Erroneous initiation (2); Helix (12); Metal binding (41); Propeptide (1); Repeat (2); Sequence conflict (1); Turn (8) |
| Keywords | 3D-structure;Calcium;Direct protein sequencing;Hydrolase;Metal-binding;Metalloprotease;Protease;Repeat;Secreted;Toxin;Virulence;Zinc;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (5) |
| Cross Reference PDB | 1AF0; 1SAT; 1SMP; 4I35; 5D7W; |
| Mapped Pubmed ID | 26750478; 9125517; |
| Motif | |
| Gene Encoded By | |
| Mass | 52,105 |
| Kinetics | |
| Metal Binding | METAL 192; /note=Zinc; catalytic; METAL 196; /note=Zinc; catalytic; METAL 202; /note=Zinc; catalytic; METAL 232; /note=Zinc; catalytic; METAL 269; /note=Calcium 1; via carbonyl oxygen; METAL 271; /note=Calcium 1; via carbonyl oxygen; METAL 273; /note=Calcium 1; METAL 301; /note=Calcium 1; METAL 303; /note=Calcium 1; via carbonyl oxygen; METAL 304; /note=Calcium 2; via carbonyl oxygen; METAL 306; /note=Calcium 1; METAL 306; /note=Calcium 2; METAL 343; /note=Calcium 2; via carbonyl oxygen; METAL 345; /note=Calcium 2; METAL 350; /note=Calcium 3; via carbonyl oxygen; METAL 352; /note=Calcium 3; via carbonyl oxygen; METAL 354; /note=Calcium 3; METAL 359; /note=Calcium 4; via carbonyl oxygen; METAL 361; /note=Calcium 4; via carbonyl oxygen; METAL 363; /note=Calcium 4; METAL 367; /note=Calcium 3; via carbonyl oxygen; METAL 368; /note=Calcium 5; via carbonyl oxygen; METAL 369; /note=Calcium 3; via carbonyl oxygen; METAL 370; /note=Calcium 5; via carbonyl oxygen; METAL 372; /note=Calcium 3; METAL 372; /note=Calcium 5; METAL 376; /note=Calcium 4; via carbonyl oxygen; METAL 377; /note=Calcium 6; via carbonyl oxygen; METAL 378; /note=Calcium 4; via carbonyl oxygen; METAL 379; /note=Calcium 6; via carbonyl oxygen; METAL 381; /note=Calcium 4; METAL 381; /note=Calcium 6; METAL 385; /note=Calcium 5; via carbonyl oxygen; METAL 386; /note=Calcium 7; via carbonyl oxygen; METAL 387; /note=Calcium 5; via carbonyl oxygen; METAL 388; /note=Calcium 7; via carbonyl oxygen; METAL 390; /note=Calcium 5; METAL 390; /note=Calcium 7; METAL 399; /note=Calcium 6; METAL 406; /note=Calcium 6; METAL 416; /note=Calcium 7 |
| Rhea ID | |
| Cross Reference Brenda |