| IED ID | IndEnz0002008411 |
| Enzyme Type ID | protease008411 |
| Protein Name |
Disease resistance protein RPS5 Resistance to Pseudomonas syringae protein 5 pNd3/pNd10 |
| Gene Name | RPS5 At1g12220 T28K15.5 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MGGCFSVSLPCDQVVSQFSQLLCVRGSYIHNLSKNLASLQKAMRMLKARQYDVIRRLETEEFTGRQQRLSQVQVWLTSVLIIQNQFNDLLRSNEVELQRLCLCGFCSKDLKLSYRYGKRVIMMLKEVESLSSQGFFDVVSEATPFADVDEIPFQPTIVGQEIMLEKAWNRLMEDGSGILGLYGMGGVGKTTLLTKINNKFSKIDDRFDVVIWVVVSRSSTVRKIQRDIAEKVGLGGMEWSEKNDNQIAVDIHNVLRRRKFVLLLDDIWEKVNLKAVGVPYPSKDNGCKVAFTTRSRDVCGRMGVDDPMEVSCLQPEESWDLFQMKVGKNTLGSHPDIPGLARKVARKCRGLPLALNVIGEAMACKRTVHEWCHAIDVLTSSAIDFSGMEDEILHVLKYSYDNLNGELMKSCFLYCSLFPEDYLIDKEGLVDYWISEGFINEKEGRERNINQGYEIIGTLVRACLLLEEERNKSNVKMHDVVREMALWISSDLGKQKEKCIVRAGVGLREVPKVKDWNTVRKISLMNNEIEEIFDSHECAALTTLFLQKNDVVKISAEFFRCMPHLVVLDLSENQSLNELPEEISELASLRYFNLSYTCIHQLPVGLWTLKKLIHLNLEHMSSLGSILGISNLWNLRTLGLRDSRLLLDMSLVKELQLLEHLEVITLDISSSLVAEPLLCSQRLVECIKEVDFKYLKEESVRVLTLPTMGNLRKLGIKRCGMREIKIERTTSSSSRNKSPTTPCFSNLSRVFIAKCHGLKDLTWLLFAPNLTFLEVGFSKEVEDIISEEKAEEHSATIVPFRKLETLHLFELRGLKRIYAKALHFPCLKVIHVEKCEKLRKLPLDSKSGIAGEELVIYYGEREWIERVEWEDQATQLRFLPSSRWRWRET |
| Enzyme Length | 889 |
| Uniprot Accession Number | O64973 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Disease resistance (R) protein that specifically recognizes the avrPphB type III effector avirulence protein from Pseudomonas syringae. Also confers resistance against Hyaloperonospora parasitica (downy mildew). Resistance proteins guard the plant against pathogens that contain an appropriate avirulence protein via an indirect interaction with this avirulence protein. That triggers a defense system including the hypersensitive response, which restricts the pathogen growth. Requires PBS1 to trigger the defense reaction against avrPphB. In case of infection by Pseudomonas syringae, AvrPphB triggers RPS5-mediated defense mechanism via the cleavage of PBS1, suggesting that the cleavage of PBS1 could trigger an exchange of ADP for ATP, thereby activating RPS5. May function as a fine-tuned sensor of alterations in the structure of the effector target PBS1. {ECO:0000269|PubMed:12947197, ECO:0000269|PubMed:17277084, ECO:0000269|PubMed:22372664, ECO:0000269|PubMed:9724691}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 183..190; /note=ATP; /evidence=ECO:0000255 |
| Features | Chain (1); Coiled coil (1); Domain (1); Initiator methionine (1); Lipidation (2); Mutagenesis (8); Nucleotide binding (1); Repeat (6); Sequence conflict (10) |
| Keywords | ATP-binding;Cell membrane;Coiled coil;Hypersensitive response;Leucine-rich repeat;Lipoprotein;Membrane;Myristate;Nucleotide-binding;Palmitate;Plant defense;Reference proteome;Repeat |
| Interact With | Q9FE20; Itself |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor {ECO:0000269|PubMed:22046960, ECO:0000269|PubMed:22331412}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10101179; 10224270; 10571892; 11226196; 11277440; 11359614; 11846877; 12172007; 12582885; 12912986; 19525323; 19659661; 20413097; 21398259; 21726371; 23144618; 24225654; 24453975; 25043057; 25859662; 26912853; 27006483; 28710392; 28973974; 32086363; 32267815; 32284014; 33982335; 8589418; 9707643; |
| Motif | |
| Gene Encoded By | |
| Mass | 101,802 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |