IED Industry Enzyme Database

Detail Information for IndEnz0002008438
IED ID IndEnz0002008438
Enzyme Type ID protease008438
Protein Name Sodium-dependent dopamine transporter
DA transporter
DAT
Solute carrier family 6 member 3
Gene Name SLC6A3 DAT1
Organism Homo sapiens (Human)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human)
Enzyme Sequence MSKSKCSVGLMSSVVAPAKEPNAVGPKEVELILVKEQNGVQLTSSTLTNPRQSPVEAQDRETWGKKIDFLLSVIGFAVDLANVWRFPYLCYKNGGGAFLVPYLLFMVIAGMPLFYMELALGQFNREGAAGVWKICPILKGVGFTVILISLYVGFFYNVIIAWALHYLFSSFTTELPWIHCNNSWNSPNCSDAHPGDSSGDSSGLNDTFGTTPAAEYFERGVLHLHQSHGIDDLGPPRWQLTACLVLVIVLLYFSLWKGVKTSGKVVWITATMPYVVLTALLLRGVTLPGAIDGIRAYLSVDFYRLCEASVWIDAATQVCFSLGVGFGVLIAFSSYNKFTNNCYRDAIVTTSINSLTSFSSGFVVFSFLGYMAQKHSVPIGDVAKDGPGLIFIIYPEAIATLPLSSAWAVVFFIMLLTLGIDSAMGGMESVITGLIDEFQLLHRHRELFTLFIVLATFLLSLFCVTNGGIYVFTLLDHFAAGTSILFGVLIEAIGVAWFYGVGQFSDDIQQMTGQRPSLYWRLCWKLVSPCFLLFVVVVSIVTFRPPHYGAYIFPDWANALGWVIATSSMAMVPIYAAYKFCSLPGSFREKLAYAIAPEKDRELVDRGEVRQFTLRHWLKV
Enzyme Length 620
Uniprot Accession Number Q01959
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number
Enzyme Function FUNCTION: Amine transporter (PubMed:1406597, PubMed:8302271, PubMed:15505207). Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals (By similarity). Regulator of light-dependent retinal hyaloid vessel regression, downstream of OPN5 signaling (By similarity). {ECO:0000250|UniProtKB:P23977, ECO:0000250|UniProtKB:Q61327, ECO:0000269|PubMed:1406597, ECO:0000269|PubMed:15505207, ECO:0000269|PubMed:8302271}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Chain (1); Disulfide bond (2); Glycosylation (3); Metal binding (9); Natural variant (6); Region (1); Sequence conflict (2); Site (1); Topological domain (3); Transmembrane (12)
Keywords Cell membrane;Cell projection;Disease variant;Disulfide bond;Dystonia;Glycoprotein;Membrane;Metal-binding;Neurodegeneration;Neurotransmitter transport;Parkinsonism;Reference proteome;Sodium;Symport;Transmembrane;Transmembrane helix;Transport
Interact With P14416; O15354; Q99578; P37840; Q5T9L3; Q9EP80; Q5BJQ5
Induction
Subcellular Location SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:1406597, ECO:0000269|PubMed:15505207, ECO:0000269|PubMed:8302271}; Multi-pass membrane protein {ECO:0000269|PubMed:15505207}. Cell projection, neuron projection {ECO:0000250|UniProtKB:P23977}. Cell projection, axon {ECO:0000269|PubMed:17296554}. Note=Localizes to neurite tips in neuronal cells (By similarity). Colocalizes with SEPTIN4 at axon terminals, especially at the varicosities (By similarity). {ECO:0000250|UniProtKB:P23977, ECO:0000250|UniProtKB:Q61327}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 11104840; 11140838; 11220753; 11244477; 11378848; 11449401; 11505915; 11558224; 11774209; 11798784; 11803442; 11803445; 11807408; 11820798; 11834311; 11911442; 11959130; 11989984; 12149916; 12172219; 12173460; 12210886; 12215242; 12232785; 12401557; 12422069; 12429746; 12453630; 12457396; 12459514; 12464618; 12465073; 12490667; 12513948; 12579508; 12605102; 12630565; 12650952; 12658362; 12660802; 12686408; 12707931; 12707943; 12708251; 12740596; 12773538; 12815660; 12874502; 12898575; 12915833; 12931138; 14505070; 14519759; 14570538; 14584093; 14597628; 14660644; 14681904; 14685824; 14732464; 14732864; 14978671; 15024013; 15024426; 15069467; 15091313; 15094788; 15135042; 15148564; 15190232; 15193761; 15202772; 15210531; 15211638; 15229055; 15274029; 15370155; 15380858; 15381926; 15542698; 15567077; 15602501; 15605097; 15608954; 15683546; 15708296; 15722958; 15724142; 15763138; 15768394; 15809660; 15823165; 15827573; 15845322; 15872345; 15924135; 15940306; 15993876; 15996968; 16041244; 16082688; 16082693; 16103889; 16109712; 16110277; 16123773; 16125912; 16139170; 16165273; 16168728; 16171832; 16178930; 16178931; 16201143; 16216085; 16222334; 16261167; 16309561; 16342279; 16368111; 16389200; 16389711; 16402340; 16406146; 16424823; 16526026; 16526040; 16537431; 16569110; 16585476; 16604301; 16611807; 16623961; 16671078; 16674552; 16702979; 16710758; 16712949; 16741944; 16760197; 16782077; 16783497; 16818870; 16894395; 16917950; 16930369; 16963468; 17023870; 17028370; 17042887; 17044099; 17044101; 17063150; 17079728; 17109713; 17120049; 17130883; 17135598; 17150335; 17157268; 17171650; 17175058; 17187291; 17191306; 17192955; 17225991; 17245411; 17250655; 17255098; 17260170; 17264803; 17267664; 17314918; 17316573; 17325714; 17380124; 17392738; 17403983; 17413453; 17427187; 17427194; 17432702; 17439486; 17440935; 17440951; 17440978; 17449448; 17449559; 17466074; 17483451; 17485610; 17500623; 17501935; 17508355; 17508996; 17525955; 17526637; 17539957; 17549062; 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22162768; 22163275; 22177721; 22182296; 22279524; 22342155; 22366190; 22408582; 22427685; 22435397; 22438288; 22454241; 22494359; 22514303; 22551036; 22564179; 22646917; 22682503; 22683321; 22689927; 22691013; 22698582; 22711722; 22723947; 22733030; 22745713; 22749356; 22778840; 22810728; 22819977; 22832428; 22844499; 22930791; 22939506; 22958175; 23032071; 23034259; 23061530; 23065263; 23076524; 23128675; 23137414; 23153115; 23161550; 23185343; 23215821; 23255304; 23273726; 23340505; 23363412; 23398760; 23524636; 23527493; 23541676; 23588108; 23597562; 23645138; 23647133; 23653272; 23681294; 23704939; 23731290; 23761898; 23808292; 23861813; 23911780; 23963056; 23968837; 23979605; 23987116; 24008096; 24024899; 24035787; 24057505; 24061496; 24064458; 24078578; 24193094; 24211691; 24274990; 24403155; 24439516; 24461309; 24487615; 24521003; 24613933; 24625311; 24633632; 24657591; 24680725; 24696011; 24708432; 24723432; 24753048; 24780147; 24824713; 24849661; 24880859; 24889756; 24911152; 24946437; 25019689; 25038551; 25093598; 25109468; 25130782; 25179220; 25212851; 25237117; 25247548; 25271118; 25313507; 25326467; 25326468; 25331903; 25339174; 25411502; 25439653; 25468771; 25496873; 25547317; 25555995; 25617181; 25656223; 25656446; 25683821; 25684044; 25687785; 25695767; 25747272; 25753457; 25804664; 25805645; 25840828; 25850298; 25850966; 25862379; 25915480; 25924976; 25963949; 26006708; 26041607; 26048990; 26058807; 26096461; 26120847; 26149737; 26160290; 26206710; 26245713; 26255829; 26305376; 26342565; 26350252; 26471374; 26478196; 26504078; 26514267; 26559242; 26593110; 26613685; 26621748; 26707863; 26729744; 26786096; 26831905; 26918589; 26935821; 26984412; 26986136; 26991370; 27045841; 27049476; 27097961; 27160695; 27167478; 27219321; 27250920; 27268567; 27287604; 27294367; 27327562; 27353511; 27490263; 27547496; 27555326; 27663598; 27676100; 27786532; 27860344; 27873141; 27882466; 27966610; 27986813; 28055236; 28101524; 28139629; 28176268; 28182634; 28234207; 28278565; 28349825; 28376280; 28393838; 28454051; 28571709; 28582390; 28584050; 28666839; 28710426; 28757070; 28797200; 28803562; 28890000; 28894302; 28983843; 29061424; 29121332; 29122229; 29145734; 29172706; 29294665; 29299388; 29332099; 29361339; 29362953; 29430519; 29433615; 29546858; 29559554; 29684537; 29978616; 29998543; 30033547; 30099719; 30153408; 30230123; 30242586; 30248905; 30259411; 30310975; 30316985; 30317684; 30472966; 30531858; 30587577; 30695550; 30705091; 30755521; 30923918; 30991630; 31067250; 31087723; 31142432; 31185047; 31291231; 31325142; 31440993; 31586043; 32077500; 32277231; 32371614; 32415851; 32554184; 32557146; 33022465; 33138218; 33287325; 33469789; 33579026; 33636518; 33667855; 33887543; 34015332; 34118233; 34199792; 9149327;
Motif
Gene Encoded By
Mass 68,495
Kinetics
Metal Binding METAL 75; /note=Sodium 1; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q7K4Y6; METAL 77; /note=Sodium 2; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q7K4Y6; METAL 78; /note=Sodium 1; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q7K4Y6; METAL 82; /note=Sodium 2; /evidence=ECO:0000250|UniProtKB:Q7K4Y6; METAL 321; /note=Sodium 2; /evidence=ECO:0000250|UniProtKB:Q7K4Y6; METAL 353; /note=Sodium 2; /evidence=ECO:0000250|UniProtKB:Q7K4Y6; METAL 418; /note=Sodium 1; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q7K4Y6; METAL 421; /note=Sodium 1; /evidence=ECO:0000250|UniProtKB:Q7K4Y6; METAL 422; /note=Sodium 1; /evidence=ECO:0000250|UniProtKB:Q7K4Y6
Rhea ID
Cross Reference Brenda