| IED ID | IndEnz0002008463 |
| Enzyme Type ID | protease008463 |
| Protein Name |
Serine protease SPPA, chloroplastic EC 3.4.21.- Signal peptide peptidase SPPA |
| Gene Name | SPPA SPPA1 At1g73990 F2P9.14 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MAKLLLLHAPHVIPRFSSSSSRSLVSAAALYRRPLLVNPQFSHIGPRLHSPYNRRFSARAFDDSPASSAEMEKEKQEQLLDGVSGKKDEDYPTGEMEYENRNAWEIFVVKFRMLFAYPWQRVRKGSVLTMTLRGQISDQLKSRFNSGLSLPQLSENFVKAAYDPRIAGVYLHIDPLSCGWGKVEEIRRHILNFKKSGKFIVGYISICGLKEYYLGCACNELFAPPSAYSFLYGLTVQASFLGGVFEKVGIEPQVQRIGKYKSAGDQLSRKSISEENYEMLSVLLDNIYSNWLDGVSDATGKKREDVENFINQGVYEIEKLKEAGLIKDIRYDDEVITMLKERLGVEKDKKLPTVDYKKYSGVKKWTLGLTGGRDQIAIIRAGGSISRVKGPLSTPGSAIIAEQLIEKIRSVRESKKYKAAIIRIDSPGGDALASDLMWREIKLLAETKPVIASMSDVAASGGYYMAMAANAIVAENLTLTGSIGVVTARFTLAKLYEKIGFNKETISRGKYAELLGAEERPLKPEEAELFEKSAQHAYQLFRDKAALSRSMPVDKMEEVAQGRVWTGKDAHSRGLIDAVGGLSRAIAIAKQKANIPLNKKVTLVELSRPSTSLPDILSGIGSSVIGVDRTLKGLLDELTITEGVQARMDGIMFQQLGRDSLATPIIDMLKDYLSSLR |
| Enzyme Length | 677 |
| Uniprot Accession Number | Q9C9C0 |
| Absorption | |
| Active Site | ACT_SITE 460; /note=Nucleophile; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Serine protease that may be involved in the light-dependent degradation of antenna and photosystem II in chloroplasts. May function during high light acclimation in plastids. {ECO:0000269|PubMed:11443110, ECO:0000269|PubMed:19349419}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Compositional bias (1); Region (1); Sequence caution (3); Transit peptide (1) |
| Keywords | Chloroplast;Hydrolase;Membrane;Plastid;Protease;Reference proteome;Serine protease;Stress response;Thylakoid;Transit peptide |
| Interact With | |
| Induction | INDUCTION: By high light. {ECO:0000269|PubMed:11443110}. |
| Subcellular Location | SUBCELLULAR LOCATION: Plastid, chloroplast stroma {ECO:0000269|PubMed:11443110}. Plastid, chloroplast thylakoid membrane {ECO:0000269|PubMed:11443110}. Note=Mostly exposed to the stroma but behaves as an intrinsic membrane protein. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 12185496; 14576160; 15028209; 15272873; 16766689; 16829591; 18067581; 18431481; 19168645; 32686366; |
| Motif | |
| Gene Encoded By | |
| Mass | 75,095 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |