| IED ID | IndEnz0002008471 |
| Enzyme Type ID | protease008471 |
| Protein Name |
Protease 4 EC 3.4.21.- Endopeptidase IV Protease IV Signal peptide peptidase |
| Gene Name | sppA b1766 JW1755 |
| Organism | Escherichia coli (strain K12) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli Escherichia coli (strain K12) |
| Enzyme Sequence | MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQVSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVNTIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQYYLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRDDMSPAAREADSRWIGELWQNYLNTVAANRQIPAEQVFPGAQGLLEGLTKTGGDTAKYALENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFANGAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAARAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGVHTDGVSTSPLADVSITRALPPEAQLMMQLSIENGYKRFITLVADARHSTPEQIDKIAQGHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSGSVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANMR |
| Enzyme Length | 618 |
| Uniprot Accession Number | P08395 |
| Absorption | |
| Active Site | ACT_SITE 209; /note=Proton donor/acceptor; /evidence=ECO:0000305; ACT_SITE 409; /note=Nucleophile |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by serine hydrolase inhibitor FP-biotin and by antipain. {ECO:0000269|PubMed:3522590}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Digests cleaved signal peptides in vitro, its in vivo function is unknown. This activity is necessary to maintain proper secretion of mature proteins across the membrane. {ECO:0000269|PubMed:18164727, ECO:0000269|PubMed:18476724, ECO:0000269|PubMed:21810987, ECO:0000269|PubMed:3522590}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Beta strand (22); Chain (1); Helix (20); Mutagenesis (7); Natural variant (6); Sequence conflict (2); Topological domain (2); Transmembrane (1); Turn (4) |
| Keywords | 3D-structure;Cell inner membrane;Cell membrane;Hydrolase;Membrane;Protease;Reference proteome;Serine protease;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:18476724, ECO:0000269|PubMed:3522590}; Single-pass membrane protein {ECO:0000269|PubMed:18476724, ECO:0000269|PubMed:3522590}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (2) |
| Cross Reference PDB | 3BEZ; 3BF0; |
| Mapped Pubmed ID | 16606699; 16858726; 7040383; |
| Motif | |
| Gene Encoded By | |
| Mass | 67,219 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |