| IED ID | IndEnz0002008480 |
| Enzyme Type ID | protease008480 |
| Protein Name |
Signal peptide peptidase-like 3 SPP-like 3 EC 3.4.23.- Intramembrane protease 2 IMP-2 Presenilin-like protein 4 |
| Gene Name | Sppl3 Imp2 Psl4 Usmg3 |
| Organism | Mus musculus (Mouse) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse) |
| Enzyme Sequence | MAEQTYSWAYSLVDSSQVSTFLISILLIVYGSFRSLNMDFENQDKEKDSNSSSGSFNGNSTNNSIQTIDSTQALFLPIGASVSLLVMFFFFDSVQVVFTICTAVLATIAFAFLLLPMCQYLTRPCSPQNKISFGCCGRFTAAELLSFSLSVMLVLIWVLTGHWLLMDALAMGLCVAMIAFVRLPSLKVSCLLLSGLLIYDVFWVFFSAYIFNSNVMVKVATQPADNPLDVLSRKLHLGPNVGRDVPRLSLPGKLVFPSSTGSHFSMLGIGDIVMPGLLLCFVLRYDNYKKQASGDSCGAPGPANISGRMQKVSYFHCTLIGYFVGLLTATVASRIHRAAQPALLYLVPFTLLPLLTMAYLKGDLRRMWSEPFHSKSSSSRFLEV |
| Enzyme Length | 384 |
| Uniprot Accession Number | Q9CUS9 |
| Absorption | |
| Active Site | ACT_SITE 200; /evidence=ECO:0000250|UniProtKB:P49810; ACT_SITE 271; /evidence=ECO:0000250|UniProtKB:P49810 |
| Activity Regulation | ACTIVITY REGULATION: Its proteolytic activity is blocked by a signal peptide peptidase (SPP) inhibitor, (ZLL)2-ketone (ZLL) or a gamma-secretase inhibitor, LY411,575. {ECO:0000250|UniProtKB:Q8TCT6}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.23.- |
| Enzyme Function | FUNCTION: Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane protein substrates in or close to their luminal transmembrane domain boundaries. Acts like a sheddase by mediating the proteolytic release and secretion of active site-containing ectodomains of glycan-modifiying glycosidase and glycosyltransferase enzymes such as MGAT5, B4GAT1 and B4GALT1 (PubMed:25354954, PubMed:25827571). Plays a role in the regulation of cellular glycosylation processes (PubMed:25354954). Required to link T-cell antigen receptor (TCR) and calcineurin-NFAT signaling cascades in lymphocytes by promoting the association of STIM1 and ORAI1 during store-operated calcium entry (SOCE) in a protease-independent manner (PubMed:25384971). {ECO:0000269|PubMed:25354954, ECO:0000269|PubMed:25384971, ECO:0000269|PubMed:25827571}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Erroneous initiation (1); Motif (1); Mutagenesis (2); Sequence conflict (2); Topological domain (10); Transmembrane (9) |
| Keywords | Endoplasmic reticulum;Golgi apparatus;Hydrolase;Membrane;Protease;Reference proteome;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q8TCT6}; Multi-pass membrane protein {ECO:0000250|UniProtKB:Q8TCT6}. Golgi apparatus {ECO:0000250|UniProtKB:Q8TCT6}. Membrane {ECO:0000250|UniProtKB:Q8TCT6}; Multi-pass membrane protein {ECO:0000250|UniProtKB:Q8TCT6}; Lumenal side {ECO:0000250|UniProtKB:Q8TCT6}. |
| Modified Residue | |
| Post Translational Modification | PTM: Not glycosylated. {ECO:0000250|UniProtKB:Q8TCT6}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11044607; 11217851; 12466851; 12904583; 14610273; 15582152; 16602821; 18799693; 21267068; 21677750; 26851218; |
| Motif | MOTIF 341..343; /note=PAL |
| Gene Encoded By | |
| Mass | 42,261 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.4.23.B24; |