| IED ID | IndEnz0002008483 |
| Enzyme Type ID | protease008483 |
| Protein Name |
Stromal processing peptidase, chloroplastic EC 3.4.24.- Chloroplast processing enzyme |
| Gene Name | SPP CPE At5g42390 MDH9.8 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MASSSSSIFTGVKFSPILAPFNSGDSRRSRYLKDSRNKVRFNPSSPRLTPHRVRVEAPSLIPYNGLWAAQPNSHKGRLKRNIVSGKEATGISLSQGRNFCLTCKRNQAGIRRALPSAFVDRTAFSLSRSSLTSSLRKHSQIVNATLGPDEPHAAGTAWPDGIVAERQDLDLLPPEIDSAELEAFLGCELPSHPKLHRGQLKNGLRYLILPNKVPPNRFEAHMEVHVGSIDEEEDEQGIAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTHTKDSEDDLFPSVLDALNEIAFHPKFLSSRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLGRRFPIGLEEQIKKWDVDKIRKFHERWYFPANATLYIVGDIDNIPRIVHNIEAVFGKNGLDNESTPSSPSPGAFGAMANFLVPKLPAGLGGTFSNEKTNTADQSKMIKRERHAIRPPVEHNWSLPGTSVDLKPPQIFKHELLQNFAINMFCKIPVSKVQTFGDLRNVLMKRIFLSALHFRINTRYKSSNPPFTSVELDHSDSGREGCTVTTLTVTAEPQNWQNAVKVAVQEVRRLKEFGVTRGELTRYMDALLKDSEHLAAMIDNVSSVDNLDFIMESDALSHTVMDQTQGHETLVAVAGTVTLEEVNTVGAKVLEFISDFGRPTAPLPAAIVACVPTKVHVDGVGESDFNISPDEIIESVKSGLLAPIEAEPELEVPKELISQSQLKELTLQRNPCFVPIPGSGLTKLHDKETGITQLRLSNGIAVNYKKSTTESRAGVMRLIVGGGRAAETSDSKGAVVVGVRTLSEGGRVGDFSREQVELFCVNHLINCSLESTEEFIAMEFRFTLRDNGMQAAFQLLHMVLERSVWLEDAFDRARQLYLSYFRSIPKSLERATAHKLMIAMLNGDERFVEPTPKSLQSLNLESVKDAVMSHFVGDNMEVSIVGDFSEEEIERCILDYLGTVKASHDSAKPPGSEPILFRQPTAGLQFQQVFLKDTDERACAYIAGPAPNRWGFTVDGDDLFQSVSKLPVAHDGLLKSEEQLLEGGDRELQKKLRAHPLFFGVTMGLLAEIINSRLFTTVRDSLGLTYDVSFELNLFDRLNLGWYVISVTSTPGKVYKAVDACKSVLRGLHSNQIAPRELDRAKRTLLMRHEAELKSNAYWLNLLAHLQASSVPRKELSCIKELVSLYEAASIEDIYLAYNQLRVDEDSLYSCIGIAGAQAGEEITVLSEEEEPEDVFSGVVPVGRGSSMTTRPTT |
| Enzyme Length | 1265 |
| Uniprot Accession Number | Q9FIH8 |
| Absorption | |
| Active Site | ACT_SITE 243; /note=Proton acceptor; /evidence=ECO:0000305; ACT_SITE 314; /evidence=ECO:0000305 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.24.- |
| Enzyme Function | FUNCTION: Cleaves presequences (transit peptides) from chloroplastic protein precursors (PubMed:12795700). Initially recognizes a precursor by binding to the C-terminus of its transit peptide and then removes the transit peptide in a single endoproteolytic step. In a next step, pursues the cleavage of transit peptide to a subfragment form (By similarity). {ECO:0000250|UniProtKB:Q40983, ECO:0000269|PubMed:12795700}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Metal binding (3); Sequence conflict (4); Transit peptide (1) |
| Keywords | Chloroplast;Hydrolase;Metal-binding;Metalloprotease;Plastid;Protease;Reference proteome;Transit peptide;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Plastid, chloroplast stroma {ECO:0000250|UniProtKB:Q40983}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 12383089; 14576160; 14671022; 15561727; 18431481; 18633119; 18775970; 23346366; 24023856; 26371235; 27247031; 30833711; 31334862; 32344669; |
| Motif | |
| Gene Encoded By | |
| Mass | 140,607 |
| Kinetics | |
| Metal Binding | METAL 240; /note=Zinc; /evidence=ECO:0000305; METAL 244; /note=Zinc; /evidence=ECO:0000305; METAL 321; /note=Zinc; /evidence=ECO:0000305 |
| Rhea ID | |
| Cross Reference Brenda | 3.4.21.102; |