| IED ID | IndEnz0002008485 |
| Enzyme Type ID | protease008485 |
| Protein Name |
Stromal processing peptidase, chloroplastic EC 3.4.24.- |
| Gene Name | OsI_23765 |
| Organism | Oryza sativa subsp. indica (Rice) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Oryzoideae Oryzeae Oryzinae Oryza Oryza sativa (Rice) Oryza sativa subsp. indica (Rice) |
| Enzyme Sequence | MASFPSPPLAAAAAAAPPRLAPGLPLAAAAVRRPSSLARRSSIALAAPANPLRCIHRRAVSPRLRRRTEAVGAASAAIGSLGEEREGCLSCFPRGRRRGRPGLARFAPCALPHTYGLSSLHSGLTGAKIRRRHVLHAAGPDEPHVASPTWSETALDKHYVDQPIGKEELEGFLNTPLPSHPKLVRGQLKNGLRYLILPNKVPANRFEAHMEVHVGSIDEEEDEQGIAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTKTKEYGEDLLPSVLDALNEIAFHPKFSSSRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSERFPIGLEEQIHKWDPDKIRRFHERWYYPANATLYLVGEINDIPRAIREIEAVFEHTLPEGEAAPMSTASPFGAMASLFAPKLPGGLAASLTGERSPAADKIKPVKRERQAIRPPVEHKWSLPGVAQDAKPPAIFQHELIQSFSINMFCKIPVNQVQTYKDLRSVLMKRIFLSALHFRINTRYKSSNPPFTSVELDHSDSGREGCTVTTLTVTAEPQNWRSAIKVAVHEVRRLKEFGVTMGEMTRYMDALIKDSEQLAMMIDSVPSVDNLDFIMESDALRHTVMDQLQGHESLLAVAETVTLEEVNTVGAEVLEFISDYGKPDAPLPAAIVACVPKKVHMDGVGETDFEIHPEEITDSIKAGLEEPIYPEPELEVPKELITQSELEDLKLQRKPSFASLSKEENVVKIFDDETGIAQRRLSNGISINYKITQNEARVGVMRLIVGGGRATEDSESKGSVIVGVRTLSEGGCVGNFSREQVELFCVNNLINCSLESNEEFIFMEFRFALRDNGMRAAFQLLHMVLEHNVWLEDAFDRATQLYLSYYRSIPKSLERSTAHKLMLAMLNHDERFVEPSPHSLQKLTLQSVKDAVMNQFVGDNMEVSIVGDFTEEEVESCVLDYLGTVSAPKSSKTQEHIEKISFLPFPSDLHFQQVYIKDTDERACAYIAGPAPNRWGFATEGNDLFNVIRSSSGDAQVSESANTDLTERKHNDVRSHSLFFGITLSLLAEIINSRLFTTVRDSMGLTYDVSFELNLFDKLDLGWYVIAVTSTPSKVHKAVDACKGVLRGLHSNKIVERELDRAKRTLLMKHEAETKTNAYWLGLLAHLQSSSVPRKEISCIKELTMLYESATIEDLYLAYEHLKVDESSLFACIGIAGAESGEETTDDELDMGLHGMGPIGGRGLSTMTRPTT |
| Enzyme Length | 1246 |
| Uniprot Accession Number | B8B0E2 |
| Absorption | |
| Active Site | ACT_SITE 231; /note=Proton acceptor; /evidence=ECO:0000250|UniProtKB:Q40983; ACT_SITE 302; /evidence=ECO:0000305 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.24.- |
| Enzyme Function | FUNCTION: Cleaves presequences (transit peptides) from chloroplastic protein precursors. Initially recognizes a precursor by binding to the C-terminus of its transit peptide and then removes the transit peptide in a single endoproteolytic step. In a next step, pursues the cleavage of transit peptide to a subfragment form. {ECO:0000250|UniProtKB:Q40983}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Erroneous gene model prediction (1); Metal binding (3); Mutagenesis (1); Transit peptide (1) |
| Keywords | Chloroplast;Hydrolase;Metal-binding;Metalloprotease;Plastid;Protease;Reference proteome;Transit peptide;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Plastid, chloroplast stroma {ECO:0000250|UniProtKB:Q40983, ECO:0000269|PubMed:20097911}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 138,656 |
| Kinetics | |
| Metal Binding | METAL 228; /note=Zinc; /evidence=ECO:0000250|UniProtKB:Q40983; METAL 232; /note=Zinc; /evidence=ECO:0000250|UniProtKB:Q40983; METAL 309; /note=Zinc; /evidence=ECO:0000305 |
| Rhea ID | |
| Cross Reference Brenda |