| IED ID | IndEnz0002008517 |
| Enzyme Type ID | protease008517 |
| Protein Name |
PAN2-PAN3 deadenylation complex catalytic subunit pan2 EC 3.1.13.4 PAB1P-dependent poly A -specific ribonuclease Poly A -nuclease deadenylation complex subunit 2 PAN deadenylation complex subunit 2 |
| Gene Name | pan2 AO090003000263 |
| Organism | Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Circumdati Aspergillus oryzae (Yellow koji mold) Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) |
| Enzyme Sequence | MEADWDELSRIQVPPPSPHGMPTIATAIAFDDVMELLWVGNEYGRITSFCGPELQRYTSVRAHPVSEGPVRQILFHDRGVISLSSKSVHMITRRGLTQWHITHEDMTDLRCMSFTAQLNKVIVAGCQKAMFTIDIDKGHIVDKLPTEYNYTMMKKSRYLCAATDTGSVNALSLTDFRVVKSWKAHGTAVNDMDARNDLLVTCGFSVRHLGSPIVDPLANVYDLKTLSPLPPIPFHAGAAYVRMHPKLHTTSFVASQTGQLQVVDLMNPNAINLRQANVSFMLGIDLSPSGEALAINDAECAIHLWGSPSKVHFNEMSKEVEFADVPARPPPLDWSPDTPLSMIGMPYYHERLFSAWPSHLVFEIGSPPAPIDQALIPYLRPAEIGHYAPNPKKTRRNQVENTRALANSEPALIAPKFLSEKAREQSKAKSDGLVTDAAETLAGTKLNGEAEDDPLLKYSNVEIKYSRFGVDDFDFRFYNQTKFSGLETHIANSFTNALLQLFKFIPLIRNVALQHAASACIFENCLLCEMGYLFDMLEKADGQNCQATNLLKTFGSFREASSLGLLEENLTNKSLSTSIQSVNRFFLGQISHDFRMILPSSDDLDHKLATVASESIRCMFCQKEIVRPGNSLVNELIYPAIDIKQIRRNPAYRFSNILRASIERETQNRGWCNYCRRYQQVAIRKTVHRMPLVMMLNTALNNPIYRRLWAIPGWLPEAVGLVVDAGQILCFEGEDLRMRMQNNMPGLVVYELVGVVSEIDIPEHQKAHLVSFINVSISSREPETTNKWHLFNDFLVTEVDKDEALRFNQPWKVPCVLAYQVKDARHAMDDNWKNVLDTTLLYRDWSLNGGRSVESLATLSEEEKPTPGTPVALDTEFVDLEKAEIDVKADGSQEIVRPSKSGLARVSVLRGSGTREGVPFIDDYITIKETIVDYVTQYSGIKPGDLDPRTSQHNLVPLKVAYKKLWLLLNLGCVFVGHGLASDFRKINIQVPKCQTVDTQYLFFHPGKNRRLSLRYLAWAVFKEYIQEEPTDNNQGHDSIEDARMALRLWKKFQEYEDAGVVSQILEELFREGSKLGFRPPARNGATAVLSRPGTAVTMQNNSGRNTPSTPEVTAPTASAPTTPRQGFRRSVALTPSNGSFAPGTGDFFGGSPLK |
| Enzyme Length | 1155 |
| Uniprot Accession Number | Q2ULU6 |
| Absorption | |
| Active Site | |
| Activity Regulation | ACTIVITY REGULATION: Positively regulated by the regulatory subunit pan3. {ECO:0000250, ECO:0000255|HAMAP-Rule:MF_03182}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Exonucleolytic cleavage of poly(A) to 5'-AMP.; EC=3.1.13.4; Evidence={ECO:0000255|HAMAP-Rule:MF_03182}; |
| DNA Binding | |
| EC Number | 3.1.13.4 |
| Enzyme Function | FUNCTION: Catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein pab1. PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by xrn1. May also be involved in post-transcriptional maturation of mRNA poly(A) tails. {ECO:0000255|HAMAP-Rule:MF_03182}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Compositional bias (1); Domain (2); Metal binding (4); Region (2); Repeat (2) |
| Keywords | Cytoplasm;Exonuclease;Hydrolase;Metal-binding;Nuclease;Reference proteome;Repeat;WD repeat;mRNA processing |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03182}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 129,302 |
| Kinetics | |
| Metal Binding | METAL 874; /note=Divalent metal cation; catalytic; /evidence=ECO:0000255|HAMAP-Rule:MF_03182; METAL 876; /note=Divalent metal cation; catalytic; /evidence=ECO:0000255|HAMAP-Rule:MF_03182; METAL 983; /note=Divalent metal cation; catalytic; /evidence=ECO:0000255|HAMAP-Rule:MF_03182; METAL 1042; /note=Divalent metal cation; catalytic; /evidence=ECO:0000255|HAMAP-Rule:MF_03182 |
| Rhea ID | |
| Cross Reference Brenda |