| IED ID | IndEnz0002008523 |
| Enzyme Type ID | protease008523 |
| Protein Name |
PAN2-PAN3 deadenylation complex catalytic subunit PAN2 EC 3.1.13.4 Inactive ubiquitin carboxyl-terminal hydrolase 52 PAB1P-dependent poly A -specific ribonuclease Poly A -nuclease deadenylation complex subunit 2 PAN deadenylation complex subunit 2 |
| Gene Name | PAN2 USP52 RCJMB04_3e13 |
| Organism | Gallus gallus (Chicken) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Sauropsida Sauria (diapsids) Archelosauria Archosauria Dinosauria Saurischia Theropoda Coelurosauria Aves Neognathae Galloanserae Galliformes Phasianidae (turkeys) Phasianinae Gallus Gallus gallus (Chicken) |
| Enzyme Sequence | MNFEGLDPSLAEYAPTLHPALDPVLDPHLNPSLLQNVELDPEGVPLEGIAVPESVHIVEGMYSELHTAVSEVGVPVSVSHFDLHEEMLWVGNHGGHATSFFGPTLERYSSFQVNSSDDIRQIQSLESGVLFLTKTNLKCMSRGGLIIFDYLMDEAEDMHSLLLTDSSTLLVGGLQNHVIEIDLNTVQETQKYTVEVPGITIMRQSNRFFFCGHTSGKVSLRDLRTFVVEHEFDAYSGSLSDFDVHGNLLVTCGFSSRMNGLACDRFLKVYDLRMMRATTPLQVHIDPFFLRFIPTYTSRLAIISQTGQCQFCEPTGLANPADIFHVNTVGPLIMTFDVSASKQALAFGDSEGCVHLWADSPEVTFNTYSRETDFALPCIVDTLPHLDWNQDLVPLSLIPVPLTSETLLSDWPAANSAPAPRRAPPVDPEILRTMKKVGFIGYAPNPRTKLRNQIPYRLKEADNEFDSFSQVPESPIGREEEPHLYMVAKKYRKVTIKYSKLGLEDFDFKHYNKTLFAGLEPHIPNAYCNCMIQVLYFLEPVRCLVQNHLCQKEFCLGCELGLLFHMLDLSRGDPCQGSNFLRAFRTIPEASALGLILADSDEATGKVNLGRLIQSWNRFILTQLHQETQEQEGPQAYRGAGSSTFGSSGDSVIGQLFSCEVENCSMCRCGKETVRVSSTLLFTLSYPESAEKPVKDYEFAQILKRSICLEQNTQAWCENCEKYQPTVQTRNIRCLPDVLVINCEVNSSKEADFWKTQAEYAFQRALMKRGGFEITKGKEISLGDWKELGNPEVGHSYPSVEELKNIWIPHAIKMRLTKNKELDVCNWSESDELSPNDDPESVYVYDLMATVVHILDSRTGGSLVGHIKVGETYHQRKEGVTHQQWYLFNDFLIEPVDKCEAVQFDMSWKVPAILYYARRNLNSKYNLVIKNPIEASVLLAEASLARKQRKCHATFIPLMLSEMPQAGDLVGLDAEFVTLNEEEAELRSDGTKSTIKPSQMSVARITCVRGQGPNEGVPFIDDYISTQEQVVDYLTQYSGIKPGDLDAKISSKHLTTLKSTYLKLRFLIDVGVKFVGHGLQKDFRVINLMVPKDQVIDTVYLFHIPRKRMISLRFLAWYFLDLKIQGETHDSIEDARTALQLYRKYLELSQGGSEPDDFRKVLKALYEKGRKLDWKVPEPDSQSSPKHGAVFPPVLAL |
| Enzyme Length | 1197 |
| Uniprot Accession Number | Q5F450 |
| Absorption | |
| Active Site | |
| Activity Regulation | ACTIVITY REGULATION: Positively regulated by the regulatory subunit PAN3. {ECO:0000255|HAMAP-Rule:MF_03182}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Exonucleolytic cleavage of poly(A) to 5'-AMP.; EC=3.1.13.4; Evidence={ECO:0000255|HAMAP-Rule:MF_03182}; |
| DNA Binding | |
| EC Number | 3.1.13.4 |
| Enzyme Function | FUNCTION: Catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. {ECO:0000255|HAMAP-Rule:MF_03182}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Domain (2); Metal binding (4); Region (2); Repeat (4) |
| Keywords | Cytoplasm;Exonuclease;Hydrolase;Metal-binding;Nuclease;Nucleus;Reference proteome;Repeat;WD repeat;mRNA processing |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm, P-body {ECO:0000255|HAMAP-Rule:MF_03182}. Nucleus {ECO:0000255|HAMAP-Rule:MF_03182}. Note=Shuttles between nucleus and cytoplasm. {ECO:0000255|HAMAP-Rule:MF_03182}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 134,863 |
| Kinetics | |
| Metal Binding | METAL 973; /note=Divalent metal cation; catalytic; /evidence=ECO:0000255|HAMAP-Rule:MF_03182; METAL 975; /note=Divalent metal cation; catalytic; /evidence=ECO:0000255|HAMAP-Rule:MF_03182; METAL 1082; /note=Divalent metal cation; catalytic; /evidence=ECO:0000255|HAMAP-Rule:MF_03182; METAL 1134; /note=Divalent metal cation; catalytic; /evidence=ECO:0000255|HAMAP-Rule:MF_03182 |
| Rhea ID | |
| Cross Reference Brenda |