| IED ID | IndEnz0002008534 |
| Enzyme Type ID | protease008534 |
| Protein Name |
Alkaline protease 1 ALP EC 3.4.21.63 Aspergillopeptidase B Aspergillus proteinase B Elastase Elastinolytic serine proteinase Oryzin |
| Gene Name | alp1 alk1 alp |
| Organism | Aspergillus flavus |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Circumdati Aspergillus flavus |
| Enzyme Sequence | MQSIKRTLLLLGAVLPAVLAGPIFPHRRAPTTIPGKYIVTFKSDVDQAAIDKHTAWATDIHKRNLQRRDSSEEDLPIGIERNFKINKFAAYSGSFDEDTIAQIRQSDEVAAVEEDQVWHLFDLTTQSDAPWGLGSISHKGQPSTDYIYDTNGGEGTYAYVVDIGINVDHEEFEGRASLAYHAAGGQHVDGVGHGTHVSGTIGGKTYGVAKKANLLSVKVFVGESSSTSIILDGFNWAANDIVSKKRTGKAAINMSLGGGYSKAFNDAVENAFNEGVLSIVAAGNENTDASRTSPASAPDAFTVAAINVNNTRAYFSNYGSVVDIFAPGQNILSAWIGSNTATNTISGTSMATPHIVGLSIYLMSLEVLSSPKAVSDRIKELATRGVVSNVAGSPNLLAYNGNA |
| Enzyme Length | 403 |
| Uniprot Accession Number | P35211 |
| Absorption | |
| Active Site | ACT_SITE 162; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240; ACT_SITE 193; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240; ACT_SITE 349; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins with broad specificity, and of Bz-Arg-OEt > Ac-Tyr-OEt. Does not hydrolyze peptide amides.; EC=3.4.21.63; |
| DNA Binding | |
| EC Number | 3.4.21.63 |
| Enzyme Function | FUNCTION: Secreted alkaline protease that allows assimilation of proteinaceous substrates. {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Domain (2); Glycosylation (2); Propeptide (1); Signal peptide (1) |
| Keywords | Direct protein sequencing;Glycoprotein;Hydrolase;Protease;Secreted;Serine protease;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..21; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 42,769 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.4.21.63; |