| IED ID | IndEnz0002008564 |
| Enzyme Type ID | protease008564 |
| Protein Name |
Outer membrane protease E E protein EC 3.4.23.- |
| Gene Name | pgtE prtA STM2395 |
| Organism | Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Salmonella Salmonella enterica (Salmonella choleraesuis) Salmonella enterica I Salmonella typhimurium Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) |
| Enzyme Sequence | MKKHAIAVMMIAVFSESVYAESALFIPDVSPDSVTTSLSVGVLNGKSRELVYDTDTGRKLSQLDWKIKNVATLQGDLSWEPYSFMTLDARGWTSLASGSGHMVDHDWMSSEQPGWTDRSIHPDTSVNYANEYDLNVKGWLLQGDNYKAGVTAGYQETRFSWTARGGSYIYDNGRYIGNFPHGVRGIGYSQRFEMPYIGLAGDYRINDFECNVLFKYSDWVNAHDNDEHYMRKLTFREKTENSRYYGASIDAGYYITSNAKIFAEFAYSKYEEGKGGTQIIDKTSGDTAYFGGDAAGIANNNYTVTAGLQYRF |
| Enzyme Length | 312 |
| Uniprot Accession Number | P06185 |
| Absorption | |
| Active Site | ACT_SITE 104; /evidence=ECO:0000250; ACT_SITE 106; /evidence=ECO:0000250; ACT_SITE 226; /evidence=ECO:0000250; ACT_SITE 228; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.23.- |
| Enzyme Function | FUNCTION: Protease that can cleave T7 RNA polymerase. Specific for paired basic residues. {ECO:0000269|PubMed:2651422}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (4); Chain (1); Frameshift (1); Sequence conflict (1); Signal peptide (1) |
| Keywords | Aspartyl protease;Cell outer membrane;Hydrolase;Membrane;Protease;Reference proteome;Signal;Transmembrane;Transmembrane beta strand |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..37; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 15760453; 17888724; |
| Motif | |
| Gene Encoded By | |
| Mass | 35,055 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.4.23.49; |