| IED ID | IndEnz0002008581 |
| Enzyme Type ID | protease008581 |
| Protein Name |
Probable glutamate carboxypeptidase PLA3 EC 3.4.17.21 Protein GOLIATH Protein PLASTOCHRON3 |
| Gene Name | PLA3 GO Os03g0790600 LOC_Os03g57660 OSJNBa0087O09.17 |
| Organism | Oryza sativa subsp. japonica (Rice) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Oryzoideae Oryzeae Oryzinae Oryza Oryza sativa (Rice) Oryza sativa subsp. japonica (Rice) |
| Enzyme Sequence | MPALTRALRCALLAARVALAREKARRAFASHGEMLSQPAAALARLPPASVRVLVGFGAALLVSLVVLHRRPAARGAAGGGGPAEYDPAARFLALSEGANATIAADLRALTAGPHLAGTGAAAGAAARVLSGFRAAGLRTLTREYTPLLSYPGHASLALLRADRTLLAHLSLDEPADVGRRLVRPYHAYAPSGGAVAEAVFVNLGREEDYLTLERLGVSVRGRVAVAIRGGGYRGGVVRRAAERSAAAVLIAGHADGGVERGTVILGGPGDPLTPGWAATAGAERLDFDHEDVKRRFPAIPSMPVSGKTASAIIRTLGGPALPADWQTGVGLPVDVGGVGPGPTLVNFTYQEDRKMGMIQDIFAIIKGYEEPDRYVILGNHRDAWTYGAVDPNSGTSALLDIARRLGIMLQSGWTPRRTIILCSWDAEEFGMIGSTEWVEENLEDLQSKAVAYLNVDCAVQGIGLFAGSTPQLDNLLVDVTRQVKDPDVEGKTVHDTWNKMTGGINIERLARTDSDFAPFLHHAGIPCMDLYYGKEFPGYHTALDSYHWMEKHGDPLFLRHVAIVEIWGLLALRLADDPVLPFDYQTYASQLQEHANAFSSMMENSKWVHLLNRSIEDLSDAGLEFLKEAKKLQDQNISDGYSLMRRRLLNDRLLLAERSFLQADGLQGRGWFKHLMYSPPEDYESKLSFFPGVADAISRSSNRSAKEQQAAVRHEVRKISRAIQRAADVLRGEFSNRNESLYSSLSVAP |
| Enzyme Length | 749 |
| Uniprot Accession Number | Q852M4 |
| Absorption | |
| Active Site | ACT_SITE 427; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q04609 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Release of an unsubstituted, C-terminal glutamyl residue, typically from Ac-Asp-Glu or folylpoly-gamma-glutamates.; EC=3.4.17.21; Evidence={ECO:0000305}; |
| DNA Binding | |
| EC Number | 3.4.17.21 |
| Enzyme Function | FUNCTION: Involved in the regular timing (plastochron) of lateral organs formation. May regulate the rate of leaf initiation and the duration of vegetative phase. Seems to be redundant to the function of PLASTOCHRON1 and PLASTOCHRON2, but to act in an independent pathway. May be required for the synthesis of small signaling molecules that have a role in regulating meristem function. Involved in plant hormone homeostasis. {ECO:0000269|PubMed:19228340}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Erroneous gene model prediction (5); Glycosylation (6); Metal binding (6); Mutagenesis (1); Region (1); Sequence caution (1); Topological domain (2); Transmembrane (1) |
| Keywords | Carboxypeptidase;Cell membrane;Developmental protein;Glycoprotein;Growth regulation;Hydrolase;Membrane;Metal-binding;Metalloprotease;Protease;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000255}; Single-pass type II membrane protein {ECO:0000255}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 81,009 |
| Kinetics | |
| Metal Binding | METAL 380; /note=Zinc 1; via tele nitrogen; catalytic; /evidence=ECO:0000250|UniProtKB:Q04609; METAL 390; /note=Zinc 1; catalytic; /evidence=ECO:0000250|UniProtKB:Q04609; METAL 390; /note=Zinc 2; /evidence=ECO:0000250|UniProtKB:Q04609; METAL 428; /note=Zinc 2; /evidence=ECO:0000250|UniProtKB:Q04609; METAL 456; /note=Zinc 1; catalytic; /evidence=ECO:0000250|UniProtKB:Q04609; METAL 540; /note=Zinc 2; via tele nitrogen; /evidence=ECO:0000250|UniProtKB:Q04609 |
| Rhea ID | |
| Cross Reference Brenda | 3.4.17.11; |