| IED ID | IndEnz0002008590 |
| Enzyme Type ID | protease008590 |
| Protein Name |
26S proteasome non-ATPase regulatory subunit 4 homolog 26S proteasome regulatory subunit RPN10 AtRPN10 26S proteasome regulatory subunit S5A homolog Multiubiquitin chain-binding protein 1 AtMCB1 |
| Gene Name | RPN10 MBP1 MCB1 At4g38630 F20M13.190 T9A14.7 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MVLEATMICIDNSEWMRNGDYSPSRLQAQTEAVNLLCGAKTQSNPENTVGILTMAGKGVRVLTTPTSDLGKILACMHGLDVGGEINLTAAIQIAQLALKHRQNKNQRQRIIVFAGSPIKYEKKALEIVGKRLKKNSVSLDIVNFGEDDDEEKPQKLEALLTAVNNNDGSHIVHVPSGANALSDVLLSTPVFTGDEGASGYVSAAAAAAAAGGDFDFGVDPNIDPELALALRVSMEEERARQEAAAKKAADEAGQKDKDGDTASASQETVARTTDKNAEPMDEDSALLDQAIAMSVGDVNMSEAADEDQDLALALQMSMSGEESSEATGAGNNLLGNQAFISSVLSSLPGVDPNDPAVKELLASLPDESKRTEEEESSSKKGEDEKK |
| Enzyme Length | 386 |
| Uniprot Accession Number | P55034 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Plays a role in maintaining the structural integrity of the 19S regulatory particle (RP), subcomplex of the 26S proteasome. Plays a major role in both the direct and indirect recognition of ubiquitinated substrates of ubiquitin/26S proteasome-mediated proteolysis (UPP). Binds and presumably selects ubiquitin-conjugates for destruction. Prefers multiubiquitin chains rather than single ubiquitins, with a binding affinity for 'Lys-48'-linked ubiquitin chains. Acts as a potential docking subunit for both ubiquitin receptors RAD23s and DSK2s. Plays a role in the growth and development via the proteasome-dependent degradation of the ABA-signaling protein ABI5/DPBF1. Plays an important role for balancing cell expansion with cell proliferation rates during shoot development. {ECO:0000269|PubMed:12671091, ECO:0000269|PubMed:17971041, ECO:0000269|PubMed:19321709, ECO:0000269|PubMed:20059542, ECO:0000269|PubMed:21764993, ECO:0000269|PubMed:22751321, ECO:0000269|PubMed:8530351, ECO:0000269|PubMed:8570648, ECO:0000269|PubMed:9442033, ECO:0000269|PubMed:9741626}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Compositional bias (2); Domain (4); Modified residue (1); Region (2); Sequence conflict (2) |
| Keywords | Phosphoprotein;Proteasome;Reference proteome;Repeat |
| Interact With | Q9SII8; Q9SFT9; P0CG48 |
| Induction | |
| Subcellular Location | |
| Modified Residue | MOD_RES 263; /note=Phosphoserine; /evidence=ECO:0007744|PubMed:18433157 |
| Post Translational Modification | PTM: Phosphorylated by PI4KG4 in vitro. {ECO:0000269|PubMed:17880284}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10363660; 17432890; 17825468; 18420596; 18650403; 18775970; 19874541; 20087596; 20118269; 20687615; 22323770; 23952686; 25620024; 26004230; 26291247; 27477278; 27733337; 30955882; 8641424; 8887631; |
| Motif | |
| Gene Encoded By | |
| Mass | 40,757 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |