| IED ID | IndEnz0002008616 |
| Enzyme Type ID | protease008616 |
| Protein Name |
Serine carboxypeptidase 24 EC 3.4.16.6 Bri1 suppressor 1 Carboxypeptidase D Serine carboxypeptidase II Cleaved into: Serine carboxypeptidase 24 chain A Serine carboxypeptidase II chain A ; Serine carboxypeptidase 24 chain B Serine carboxypeptidase II chain B |
| Gene Name | SCPL24 BRS1 At4g30610 F17I23.50 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MARTHFIFLLLVALLSTTFPSSSSSREQEKDRIKALPGQPKVAFSQYSGYVNVNQSHGRALFYWLTESSSPSPHTKPLLLWLNGGPGCSSIAYGASEEIGPFRINKTGSNLYLNKFAWNKDANLLFLESPAGVGYSYTNTSSDLKDSGDERTAQDNLIFLIKWLSRFPQYKYRDFYIAGESYAGHYVPQLAKKINDYNKAFSKPIINLKGFLVGNAVTDNQYDSIGTVTYWWTHAIISDKSYKSILKYCNFTVERVSDDCDNAVNYAMNHEFGDIDQYSIYTPTCVAAQQKKNTTGFFVRMKNTLLRRRLVSGYDPCTESYAEKYFNRPDVQRAMHANVTGIRYKWTACSDVLIKTWKDSDKTMLPIYKELAASGLRIWIFSGDTDSVVPVTATRFSLSHLNLPVKTRWYPWYTDNQVGGWTEVYKGLTFATVRGAGHEVPLFEPKRALILFRSFLAGKELPRSY |
| Enzyme Length | 465 |
| Uniprot Accession Number | Q9M099 |
| Absorption | |
| Active Site | ACT_SITE 181; /evidence=ECO:0000250; ACT_SITE 386; /evidence=ECO:0000250; ACT_SITE 438; /evidence=ECO:0000250 |
| Activity Regulation | ACTIVITY REGULATION: Completely inhibited by phenylmethylsulfonyl fluoride (PMSF) and partially by leupeptin. {ECO:0000269|PubMed:16123046}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Preferential release of a C-terminal arginine or lysine residue.; EC=3.4.16.6; |
| DNA Binding | |
| EC Number | 3.4.16.6 |
| Enzyme Function | FUNCTION: Active serine carboxypeptidase with broad substrate preference, including basic and hydrophilic groups. Processes a protein involved in an early event in the brassinosteroid signaling pathway. {ECO:0000269|PubMed:11320207, ECO:0000269|PubMed:16123046}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 50 degrees Celsius. {ECO:0000269|PubMed:16123046}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 5.5. {ECO:0000269|PubMed:16123046}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (2); Disulfide bond (3); Glycosylation (6); Mutagenesis (2); Propeptide (1); Sequence conflict (1); Signal peptide (1) |
| Keywords | Carboxypeptidase;Disulfide bond;Glycoprotein;Hydrolase;Protease;Reference proteome;Secreted;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted, extracellular space {ECO:0000269|PubMed:16123046}. |
| Modified Residue | |
| Post Translational Modification | PTM: N-glycosylated. {ECO:0000269|PubMed:16123046}. |
| Signal Peptide | SIGNAL 1..24; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 16920880; 18650403; 19136719; 28441590; 28718794; |
| Motif | |
| Gene Encoded By | |
| Mass | 52,845 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |