| IED ID | IndEnz0002008631 |
| Enzyme Type ID | protease008631 |
| Protein Name |
CysO-cysteine peptidase EC 3.13.1.6 Metallocarboxypeptidase Mec |
| Gene Name | mec mec+ MT1376 |
| Organism | Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Corynebacteriales Mycobacteriaceae Mycobacterium Mycobacterium tuberculosis complex Mycobacterium tuberculosis Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) |
| Enzyme Sequence | MLLRKGTVYVLVIRADLVNAMVAHARRDHPDEACGVLAGPEGSDRPERHIPMTNAERSPTFYRLDSGEQLKVWRAMEDADEVPVVIYHSHTATEAYPSRTDVKLATEPDAHYVLVSTRDPHRHELRSYRIVDGAVTEEPVNVVEQY |
| Enzyme Length | 146 |
| Uniprot Accession Number | P9WHS0 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-Cys + H2O = [CysO sulfur-carrier protein]-C-terminal Gly-Gly + H(+) + L-cysteine; Xref=Rhea:RHEA:48732, Rhea:RHEA-COMP:12207, Rhea:RHEA-COMP:12212, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:35235, ChEBI:CHEBI:90778, ChEBI:CHEBI:90783; EC=3.13.1.6; Evidence={ECO:0000250|UniProtKB:P9WHS1}; |
| DNA Binding | |
| EC Number | 3.13.1.6 |
| Enzyme Function | FUNCTION: Protease that hydrolyzes the covalent CysO-cysteine adduct synthesized by CysM to release L-cysteine and regenerate CysO. {ECO:0000250|UniProtKB:P9WHS1}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Amino-acid biosynthesis; L-cysteine biosynthesis. |
| nucleotide Binding | |
| Features | Chain (1); Domain (1); Metal binding (3); Motif (1) |
| Keywords | Amino-acid biosynthesis;Cysteine biosynthesis;Hydrolase;Metal-binding;Metalloprotease;Protease;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 88..101; /note=JAMM motif; /evidence=ECO:0000255|PROSITE-ProRule:PRU01182 |
| Gene Encoded By | |
| Mass | 16,530 |
| Kinetics | |
| Metal Binding | METAL 88; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU01182; METAL 90; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU01182; METAL 101; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU01182 |
| Rhea ID | RHEA:48732 |
| Cross Reference Brenda |