| IED ID | IndEnz0002008649 |
| Enzyme Type ID | protease008649 |
| Protein Name |
Neutral protease 2 homolog MGG_10927 EC 3.4.24.39 Deuterolysin MGG_10927 |
| Gene Name | GLRG_10315 |
| Organism | Colletotrichum graminicola (strain M1.001 / M2 / FGSC 10212) (Maize anthracnose fungus) (Glomerella graminicola) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Hypocreomycetidae Glomerellales Glomerellaceae Colletotrichum Colletotrichum graminicola species complex Colletotrichum graminicola (Maize anthracnose fungus) (Glomerella graminicola) Colletotrichum graminicola (strain M1.001 / M2 / FGSC 10212) (Maize anthracnose fungus) (Glomerella graminicola) |
| Enzyme Sequence | MKFSIGVSLLATLAGAVNVDMAKRDTSPLNVKLEALGNSGVKAVLTNTGDSDIKLFKTGTFLDKSPVEKVEVFAAGSKIDFDGVRLQISTTGLTEEAFQIVAAGETFEVEFDAAELHDLSKGGAVDIVTQGSFLYADVDSTEIAGTIPFSSNSVHTEINGDEAASVRAAFLAKRTIVQSDCTGTRRTATVNAISRCRALAAAASQAAASGPVARMTEYFKSSTTATRNSVATVFRNIVSECGSTTSGVSRQYCTDVYGACSNGVIAYTVPAQNYMVNCPYFFNNMAAASSTCHAQDQQTTILHEMTHLRQIKGTSDYGGYGYNFVRSLSAAQNLNHADTYTLFAQSIYAGC |
| Enzyme Length | 351 |
| Uniprot Accession Number | E3QWD3 |
| Absorption | |
| Active Site | ACT_SITE 304; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Preferential cleavage of bonds with hydrophobic residues in P1'. Also 3-Asn-|-Gln-4 and 8-Gly-|-Ser-9 bonds in insulin B chain.; EC=3.4.24.39; |
| DNA Binding | |
| EC Number | 3.4.24.39 |
| Enzyme Function | FUNCTION: Secreted metalloproteinase that allows assimilation of proteinaceous substrates. Shows high activities on basic nuclear substrates such as histone and protamine (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Disulfide bond (2); Metal binding (2); Propeptide (1); Signal peptide (1) |
| Keywords | Cleavage on pair of basic residues;Disulfide bond;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Secreted;Signal;Zinc;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..16; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 37,190 |
| Kinetics | |
| Metal Binding | METAL 303; /note=Zinc; catalytic; /evidence=ECO:0000250; METAL 307; /note=Zinc; catalytic; /evidence=ECO:0000250 |
| Rhea ID | |
| Cross Reference Brenda |