| IED ID | IndEnz0002008650 |
| Enzyme Type ID | protease008650 |
| Protein Name |
Neutral protease 2 homolog AN7962 EC 3.4.24.39 Deuterolysin AN7962 |
| Gene Name | AN7962 |
| Organism | Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Nidulantes Emericella nidulans (Aspergillus nidulans) Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) |
| Enzyme Sequence | MKFIAPIALLGMFQAASASPVDIKTSNAGLQVTLSQINNTRIKAVVQNTGSEEVTFMHMNFFKDASPVKKVSIFRNNDEVEFQGIKYRVQTDDLSDEVLTSLAPGASFEDEFDIAATSDLSSGGPVTIRSEGIVPLVTEKSVTGSLSYSSNELTIDVDGAEAAKIETVGAQLSKLSKRTRVSSCSGTRATALQTALRNAASLASRAASAASQGGSTFTTFFKSDSSSTRNAVAARLRAVASESSSTSSGSTTYYCTDVYGYCSSNVLAYTLPAYNIIANCDIYYTYLPALTGTCYAQDQATTTLHEFTHAPGVYSPGTDDLGYGYDAATRLSASQALNNADSYALYANAVYLGC |
| Enzyme Length | 354 |
| Uniprot Accession Number | Q5AUR8 |
| Absorption | |
| Active Site | ACT_SITE 306; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Preferential cleavage of bonds with hydrophobic residues in P1'. Also 3-Asn-|-Gln-4 and 8-Gly-|-Ser-9 bonds in insulin B chain.; EC=3.4.24.39; |
| DNA Binding | |
| EC Number | 3.4.24.39 |
| Enzyme Function | FUNCTION: Secreted metalloproteinase that allows assimilation of proteinaceous substrates. Shows high activities on basic nuclear substrates such as histone and protamine (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Disulfide bond (2); Metal binding (3); Propeptide (1); Signal peptide (1) |
| Keywords | Cleavage on pair of basic residues;Disulfide bond;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Secreted;Signal;Zinc;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..19; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 37,423 |
| Kinetics | |
| Metal Binding | METAL 305; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 309; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 320; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
| Rhea ID | |
| Cross Reference Brenda |