| IED ID | IndEnz0002008691 |
| Enzyme Type ID | protease008691 |
| Protein Name |
Opiorphin prepropeptide Basic proline-rich lacrimal protein Proline-rich protein 1 PRL1 Cleaved into: Opiorphin |
| Gene Name | OPRPN BPLP PROL1 |
| Organism | Homo sapiens (Human) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human) |
| Enzyme Sequence | MKLTFFLGLLALISCFTPSESQRFSRRPYLPGQLPPPPLYRPRWVPPSPPPPYDSRLNSPLSLPFVPGRVPPSSFSRFSQAVILSQLFPLESIRQPRLFPGYPNLHFPLRPYYVGPIRILKPPFPPIPFFLAIYLPISNPEPQINITTADTTITTNPPTTATATTSTSTKPTMTISSSTVPISSTPEPATSISAATPAASTENTTQILANRPHTVLLNATVQVTTSNQTILSSPAFKSFWQKLFAIFG |
| Enzyme Length | 248 |
| Uniprot Accession Number | Q99935 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Opiorphin is an endogenous inhibitor of neprilysin and aminopeptidase N. Inhibits the breakdown of substance P, Mca-BK2 and Met-enkephalin by neprilysin in vitro with IC(50) values of 29 uM, 33 uM and 33 uM respectively. Inhibits the breakdown of Ala-pNA by aminopeptidase N in vitro with an IC(50) of 65 uM. Has a potent analgesic effect when administered to rats by intravenous injection. {ECO:0000269|PubMed:17101991}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Frameshift (1); Glycosylation (1); Modified residue (1); Peptide (1); Region (1); Sequence conflict (1); Signal peptide (1) |
| Keywords | Direct protein sequencing;Glycoprotein;Protease inhibitor;Pyrrolidone carboxylic acid;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:17101991}. |
| Modified Residue | MOD_RES 22; /note="Pyrrolidone carboxylic acid"; /evidence="ECO:0000269|PubMed:17101991, ECO:0000269|PubMed:25946035" |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..21; /evidence="ECO:0000269|PubMed:17101991, ECO:0000269|PubMed:25946035" |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 18410445; 33593085; |
| Motif | |
| Gene Encoded By | |
| Mass | 27,217 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |