| IED ID | IndEnz0002008712 |
| Enzyme Type ID | protease008712 |
| Protein Name |
Rhomboid-like protease 4 EC 3.4.21.105 |
| Gene Name | ROM4 |
| Organism | Toxoplasma gondii |
| Taxonomic Lineage | cellular organisms Eukaryota Sar Alveolata Apicomplexa Conoidasida Coccidia Eucoccidiorida Eimeriorina Sarcocystidae Toxoplasma Toxoplasma gondii |
| Enzyme Sequence | MVWTSAVVMASPHGSASRAGSNRRTDGLSVNLGQDNTLKHDTPTETAPSASPPRVAPAPIPPKGGPAATPGKDGALDNRGSHDSVPPVAVAIPGREEVNATASQKSVVAGANSQPTPGTQSSKAPASDVDGSSKHGSPEHPDAGSPEVNAEGVPVEEALQAIGDDDPLIHNLPDGVVGRRAPANPFNSSMYAKLRGKKKKHRPKVRDPKLNNNPLRGRLVVCISTTALLCWIYMWELIYNFTSFNGRCVSPVMYPDYKLQEAKQRQPYVIRYGYGGCEYNLGSLAYPRASFGTSAGDKGWPVDLVPNGSAGSAATSWDSPNARVLRHLGGLETNYIREYSETFRLFTSMYMHGGWLHILINLSCQIQILWIIEPDWGFLRTTLLFFLGGISGNLLSAVADPCSITVGSSGSMYALLGALIPYCVEYWKSIPRPGCILVFMIVVIIGILTGMAGFTDNYAHMGGALGGILWGFASITTVSACDKCTLGERMAMTPPFSWCVPKATQQKLLERAKARKAEAIRRRKLQAIQKKKAGGARGKAMYAVKMRLQEEGRPPCKMSFREWVIRGLCAAALFAYWLILFLYLLDPSLYKSYSPPGQLKFSGWLYCKCGTIVYQAPQTYGNLGRFWCFGSEKDAQYYLEP |
| Enzyme Length | 641 |
| Uniprot Accession Number | Q695T8 |
| Absorption | |
| Active Site | ACT_SITE 409; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 460; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.; EC=3.4.21.105; |
| DNA Binding | |
| EC Number | 3.4.21.105 |
| Enzyme Function | FUNCTION: Putative serine protease involved in intramembrane proteolysis and the subsequent release of polypeptides from their membrane anchors. {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (3); Erroneous initiation (1); Region (3); Sequence conflict (5); Transmembrane (6) |
| Keywords | Hydrolase;Membrane;Protease;Serine protease;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Membrane {ECO:0000269|PubMed:15753289}; Multi-pass membrane protein {ECO:0000269|PubMed:15753289}. Note=Uniformly distributed at the surface of intracellular and extracellular tachyzoites. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 69,660 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.4.21.105; |