| IED ID | IndEnz0002008748 |
| Enzyme Type ID | protease008748 |
| Protein Name |
Extracellular small neutral protease EC 3.4.24.77 Snapalysin |
| Gene Name | snpA |
| Organism | Streptomyces sp. (strain C5) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Streptomycetales Streptomycetaceae Streptomyces unclassified Streptomyces Streptomyces sp. (strain C5) |
| Enzyme Sequence | MRMPLSVLTAAGLSLATLGLGTAGPASATPTAEGAPVVAYDGSPSAGSPADAKAEAAANRAFFEAVLRSVAEKRAANPKSTAAVTVVYNASGAPSFATQIARGTQIWNSSVSNVRLQAGSSGVDFTYREGNDPRGSYASTDGHGRGYIFLDYRQNQTYDSTRVTAHETGHVLGLPDHYSGPCSELMSGGGPGPSCTNAYPNSAERSRVNQLWANGFAAAMDKALEKSAR |
| Enzyme Length | 229 |
| Uniprot Accession Number | P43163 |
| Absorption | |
| Active Site | ACT_SITE 167; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolyzes proteins with a preference for Tyr or Phe in the P1' position. Has no action on amino-acid p-nitroanilides.; EC=3.4.24.77; |
| DNA Binding | |
| EC Number | 3.4.24.77 |
| Enzyme Function | FUNCTION: Milk hydrolyzing. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 55 degrees Celsius.; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 7.0.; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Disulfide bond (1); Metal binding (5); Propeptide (1); Signal peptide (1) |
| Keywords | Calcium;Direct protein sequencing;Disulfide bond;Hydrolase;Metal-binding;Metalloprotease;Protease;Secreted;Signal;Zinc;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..28; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 23,607 |
| Kinetics | |
| Metal Binding | METAL 159; /note=Calcium; /evidence=ECO:0000250|UniProtKB:P56406; METAL 161; /note=Calcium; /evidence=ECO:0000250|UniProtKB:P56406; METAL 166; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:P56406; METAL 170; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:P56406; METAL 176; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:P56406 |
| Rhea ID | |
| Cross Reference Brenda |