| IED ID | IndEnz0002008772 |
| Enzyme Type ID | protease008772 |
| Protein Name |
26S proteasome regulatory subunit 6B homolog Protein YNT1 Tat-binding homolog 2 |
| Gene Name | RPT3 YNT1 YTA2 YDR394W D9509.14 |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Enzyme Sequence | MEELGIVTPVEKAVEEKPAVKSYASLLAQLNGTVNNNSALSNVNSDIYFKLKKLEKEYELLTLQEDYIKDEQRHLKRELKRAQEEVKRIQSVPLVIGQFLEPIDQNTGIVSSTTGMSYVVRILSTLDRELLKPSMSVALHRHSNALVDILPPDSDSSISVMGENEKPDVTYADVGGLDMQKQEIREAVELPLVQADLYEQIGIDPPRGVLLYGPPGTGKTMLVKAVANSTKAAFIRVNGSEFVHKYLGEGPRMVRDVFRLARENAPSIIFIDEVDSIATKRFDAQTGSDREVQRILIELLTQMDGFDQSTNVKVIMATNRADTLDPALLRPGRLDRKIEFPSLRDRRERRLIFGTIASKMSLAPEADLDSLIIRNDSLSGAVIAAIMQEAGLRAVRKNRYVILQSDLEEAYATQVKTDNTVDKFDFYK |
| Enzyme Length | 428 |
| Uniprot Accession Number | P33298 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 213..220; /note=ATP; /evidence=ECO:0000255 |
| Features | Beta strand (1); Chain (1); Cross-link (1); Helix (4); Modified residue (1); Nucleotide binding (1); Sequence conflict (2) |
| Keywords | 3D-structure;ATP-binding;Acetylation;Cytoplasm;Direct protein sequencing;Isopeptide bond;Nucleotide-binding;Nucleus;Proteasome;Reference proteome;Ubl conjugation |
| Interact With | P50086; P32628; P43588; P32496; Q04062; P33299; Itself; P53549; P33297; Q01939 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}. Nucleus {ECO:0000305}. |
| Modified Residue | MOD_RES 1; /note=N-acetylmethionine; /evidence=ECO:0000269|PubMed:12504901 |
| Post Translational Modification | PTM: N-acetylated by NAT3. {ECO:0000269|PubMed:12504901}. |
| Signal Peptide | |
| Structure 3D | Electron microscopy (27); X-ray crystallography (2) |
| Cross Reference PDB | 2DZN; 2DZO; 3JCO; 3JCP; 4CR2; 4CR3; 4CR4; 5A5B; 5MP9; 5MPA; 5MPB; 5MPC; 5WVI; 5WVK; 6EF0; 6EF1; 6EF2; 6EF3; 6FVT; 6FVU; 6FVV; 6FVW; 6FVX; 6FVY; 6J2C; 6J2N; 6J2Q; 6J2X; 6J30; |
| Mapped Pubmed ID | 10363642; 10417703; 10559920; 10664589; 10688190; 11283351; 11595789; 11742986; 11805826; 11805837; 14759368; 15135049; 15571806; 15575969; 15905137; 16284124; 16429126; 16554755; 16725216; 16922378; 17082762; 17329811; 17499717; 17555716; 17803938; 18467557; 19013276; 19289147; 19412160; 19446322; 19446323; 19516331; 19536198; 19841731; 19843524; 20074027; 20471945; 20800707; 21139140; 21211719; 21389348; 21439406; 21685082; 21734642; 21931558; 22037170; 22086954; 22195964; 22350874; 22493437; 23202731; 23217712; 23644457; 23672618; 23731471; 23770819; 23965995; 24013205; 24040173; 24598877; 24706844; 25333764; 26130806; 26182356; 26208326; 26262643; 26327695; 26365526; 26449534; 26642761; 26929360; 27677933; 28106073; 28115689; 30067984; 30309908; 30792173; 8962070; 9342402; 9430676; 9584156; 9724628; 9741626; |
| Motif | |
| Gene Encoded By | |
| Mass | 47,894 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 5.6.1.5; |