| IED ID | IndEnz0002008774 |
| Enzyme Type ID | protease008774 |
| Protein Name |
Gamma-glutamyl phosphate reductase GPR EC 1.2.1.41 Glutamate-5-semialdehyde dehydrogenase Glutamyl-gamma-semialdehyde dehydrogenase GSA dehydrogenase |
| Gene Name | proA BSU13130 |
| Organism | Bacillus subtilis (strain 168) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus subtilis group Bacillus subtilis Bacillus subtilis subsp. subtilis Bacillus subtilis (strain 168) |
| Enzyme Sequence | MSEVSVKAKLAKEAAAEMIMKTTAEKDEALSLIANGLRKELDFLLAENAKDIVNGKENGLTPDIIDRLSLDEKRIRDIADAVELLIDLADPIGDSLETIEKENGLFIEKIRVPLGVVGMIYEARPNVTVDAATLCLKTGNAVVLRGSSSAIHSNKALVSVIYRALEQSALPIHAVQLIEDTSRETAKELFTLNDGLDVLIPRGGKKLIDLVVRESTVPVLETGAGNCHIFIDETAKPQMAEKVVVNAKTQRPSVCNAIESLLIHKAWARQHGKELLDQLENAGVEIRGDELVCELHPSSKQASKEDWETEFLAPVLSVKTVENVQEAVKHIQQYGTNHSEAILTENDKNAVYFQTAVDAAAVYHNASTRFTDGFEFGYGAEIGISTQKLHARGPMGLPALTSTKYIIKGTGQIRE |
| Enzyme Length | 415 |
| Uniprot Accession Number | P39821 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=L-glutamate 5-semialdehyde + NADP(+) + phosphate = H(+) + L-glutamyl 5-phosphate + NADPH; Xref=Rhea:RHEA:19541, ChEBI:CHEBI:15378, ChEBI:CHEBI:43474, ChEBI:CHEBI:57783, ChEBI:CHEBI:58066, ChEBI:CHEBI:58274, ChEBI:CHEBI:58349; EC=1.2.1.41; Evidence={ECO:0000255|HAMAP-Rule:MF_00412}; |
| DNA Binding | |
| EC Number | 1.2.1.41 |
| Enzyme Function | FUNCTION: Catalyzes the NADPH-dependent reduction of L-glutamate 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. {ECO:0000255|HAMAP-Rule:MF_00412}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. {ECO:0000255|HAMAP-Rule:MF_00412}. |
| nucleotide Binding | |
| Features | Chain (1); Frameshift (1); Sequence conflict (4) |
| Keywords | Amino-acid biosynthesis;Cytoplasm;NADP;Oxidoreductase;Proline biosynthesis;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00412}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 45,252 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:19541 |
| Cross Reference Brenda |